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Page 1 of 5
PHARMACEUTICAL BIOTECHNOLOGY
2011-2015
(20 mark questions)
1. a) Describe the structure of Bacteria with neat diagram.
b) Microbiological assay of antibiotics.
2. a) Discuss the various types of sterilization with examples.
b) Explain the production of Monoclonal antibodies with applications
3. a) Explain the Structure and Reproduction of Fungi.
b) Sterility testing for Pharmaceutical preparations.
4. a) Define antigen and antibody. Explain the antigen antibody reactions.
b) Define Immobilization. Explain the different methodsof immobilization with applications
5. (a) Write about the morphology, reproduction andnutrition of bacteria.
(b) How an antibiotic can be assayed by one level and two level?
6. (a) Define and classify the immunity? Explain the production and evaluation of BCG
vaccine.
(b) Explain the production of insulin by genetic engineering technology.
7. (a) Discuss bacterial growth curve.
(b) Explain gram staining method.
8. Explain production of monoclonal antibody and its application.
9. a) Define disinfection. Classify them with examples and write their mechanism of action.
b) Differentiate dry heat and filtration sterilization
10.a) Production of penicillin by fermentation.
b) Poliomyelitis vaccine.
11. Define sterilization and classify them. Discuss moist heat sterilization with examples.
12. Discuss hybridoma technology with production and its application.
13. a) Differentiate between Gram positive and Gram negative cell wall with a neat labeled
diagram.
b) Define virus. Explain bacterial growth curve.
14. a) What are Mono Clonal antibodies? Explain its methods of preparation and its
application.
b) Explain the design and construction of the Fermenter.
PHARMACEUTICAL BIOTECHNOLOGY
Page 2 of 5
2011-2015
(5 mark questions)
1. Test for Sterility.
2. Evaluation of Disinfectants.
3. Microbial Biotransformation.
4. Hypersensitivity Reactions.
5. Cloning Vectors.
6. Production of Alcohol.
7. Media for Animal cell culture.
8. Methods of Immobilization.
9. Counting of Bacteria.
10. Evaluation of Disinfectants.
11. Microbial assay of Vitamins.
12. Single Cell Protein.
13. Immuno deficiency diseases.
14. Production of Insulin.
15. Production of Citric Acid.
16. Maintenance of cell culture.
17. Radiation sterilization.
18. Rideal-Walker (R.W) test.
19. ELISA technique.
20. Citric acid production by fermentation method.
21. Biosensors.
22. Application of animal tissue culture.
23. Restriction endonucleases.
24. Bacterial growth curve.
25. Autoclave.
26. Phenol coefficient test.
27. Simple media.
28. Differentiate between active immunity and passive immunity.
29. Polio vaccine.
30. Production of interferon by recombinant DNA (Deoxyribo nucleic acid)
technology.
31. Method of immobilization of enzyme.
32. Application of animal tissue culture.
33. Explain about the structures external to the cell wall.
34. Types of biotransformation reactions.
35. Auto immune disorders
36. Small pox vaccine preparation and evaluation.
37. Cloning strategies.
38. Quantitative estimation of antigen and antibody.
39. Techniques in immobilization of enzyme.
40. Screening method in fermentation process.
41. Growth curve of bacteria.
42. Gaseous sterilization.
43. Evaluation of disinfectant.
44. Gram staining.
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45. Production of citric acid by fermentation method.
46. Enzyme linked immunosorbent assay.
47. Production of insulin by recombinant DNA (Deoxyribo Nucleic Acid)
technology.
48. Types of biosensors.
49. Hot air oven
50. Preparation of diphtheria toxoid
51. Factors affecting disinfection
52. Bioprocessing of ethanol
53. Hyper sensitivity reaction
54. Microbiological assay of vitamin B12.
55. Regulation of gene expression
56. Production of Hepatitis B vaccine by rDNA technology
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PHARMACEUTICAL BIOTECHNOLOGY
2011-2015
1. Lytic and Lysogenic cycle.
2. Conjugation.
3. Functions of MHC.
4. Southern blot.
5. Gene expression.
6. Source of organisms for the production of citric acid.
7. Primary culture.
8. Biosensors.
9. Cell Line with Examples.
10. Live bacterial vaccines.
11. Bacterial Flagella.
12. Moist heat sterilization.
13. Transduction.
14. Types of Immunity.
15. Viral vaccines.
16. Western blot.
17. Restriction endonucleases.
18. Cell line with examples.
19. Microbial Biotransformation.
20. Source of organisms for the production of Vitamin B12.
21. Active immunity.
22. Anabolism.
23. Bacteriophage.
24. Biological indicator.
25. Cloning.
26. Enzyme induction.
27. Facultative anaerobe.
28. Gene mapping.
29. Haemagglutinin.
30. Interferon.
31. Define bacteria.
32. List the methods of sterilization.
33. Tyndallization.
34. Fumigation.
35. Use of culture media.
36. Types of immunity.
37. Use of western blot test.
38. High yield producing organism of glutamic acid.
39. Structure of DNA (Deoxyribo nucleic acid).
40. Culture media for animal cells.
41. Classification of micro organism based on temperature.
42. List some sterile pharmaceutical products.
43. Chick-Martin test.
44. Insertion sequences
(2 mark questions)
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45. Application of Biotransformation.
46. Haptens
47. Triple Vaccines
48. Northen blot
49. Official Parameters in fermentation process
50. Component of Biosensor
51. Define disinfectant.
52. Types of flagella.
53. Examples of gram – positive bacteria.
54. Pasterurisation.
55. Example of bacterial filters.
56. Common staining techniques
57. Examples of media used in animal cell culture
58. Define Toxoid
59. Give organism name of cholera vaccine.
60. Define Immobilization.
61. Define immunity
62. Examples of killed bacterial vaccine
63. Define Biosensor
64. Synchronous culture
65. Classify Disinfectant
66. Use of Western Blot test
67. Examples of Motile bacteria
68. Define Viable count
69. Define Sterilization
70. Define Vectors