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Reg. No:…………………………….
KIGALI INSTITUTE OF SCIENCE AND TECHNOLOGY
INSTITUT DES SCIENCES ET TECHNOLOGIE
Avenue de l'Armée, B.P. 3900 Kigali, Rwanda
INSTITUTE EXAMINATIONS – ACADEMIC YEAR 2013
END OF SEMESTER EXAMINATION (MAIN EXAM)
FACULTY OF SCIENCE
DEPARTMENT OF APPLIED CHEMISTRY
THIRD YEAR, EXAM SEM. II
CHE 3329-BIOCHEMISTRY OF MICROORGANISMS
DATE: 26/04/ 2013
TIME: 2 HOURS
MAXIMUM MARKS = 60
INSTRUCTIONS
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This paper contains FIVE (5) questions
Answer any THREE (3) questions
No written materials allowed in exam room.
Write all answers in the booklet provided.
Do not forget to write your Registration Number.
Do not write any answers on this question paper
1
SECTION A
Question 1
(a) Describe five (5) key characteristics/features for each of the following
microorganisms
(i) Viruses
(ii) Bacteria
(iii)Protozoa
(5 marks)
(5 marks)
(5 marks)
(b) State at least five (5) tests you would conduct to determine the characteristics of a
newly discovered bacterial strain.
(5 marks)
SECTION B
Question 2
(a) Explain why viral multiplication is described as a replication process but not a
reproductive process?
(5 marks)
(b) State the factors that enable viruses to multiply in blood cells but not serum.
(4 marks)
(c) State the defining characteristics of the following infectious agents
(i)
Virions
(2 marks)
(ii)
Virusoids
(2marks)
(iii) Prions
(2 marks)
(d) Explain how an animal virus penetrates a host cell.
(5 marks)
Question 3
(a) In relation to bacterial metabolism, define each of the following terms
(i)
Syntrophy
(ii)
Disproportionation
(iii) Reductive dechlorination
(iv)
Chemorganotrophy
(b) Illustrate the differences and similarities between photoautotrophs and
photoheterotrophs.
(c) Discuss the mechanism of bacterial fermentation without substrate level
phosphorylation.
Question 4
(a) State two characteristic features of the following classes of protozoa
(i)
Sarcodina
(ii)
Ciliophora
(iii) Apicomplexa
(b) Describe the life cycle of a malaria parasite.
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(2 marks)
(2 marks)
(2 marks)
(2 marks)
(4 marks)
(8 marks)
(2 marks)
(2 marks)
(2 marks)
(14 marks)