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CURRICULUM VITAE Amanda Ward Bostian T.S. (ABB) Education 2012-Present Ph.D, North Carolina State University Major: Animal Science Concentration: Reproductive Physiology Advisor: Drs. Scott Whisnant & Daniel Poole GPA: 3.50 2015 Graduate of NAPSRx Pharmacology/Pharmaceutical Sales Training Program CNPR Number 620482015 2006-2008 M.S., The University of Tennessee, Knoxville Major: Animal Science Concentration: Reproductive Physiology Minor: Statistics Co-Advisors: Dr. J. Lannett Edwards, Associate Professor Dr. F. Neal Schrick, Professor GPA: 3.75 2002-2006 B.S., North Carolina State University Major: Animal Science Concentration: Industry GPA: 3.48 Thesis/Other Publications Ward AM. Evaluating Effects of a Prostaglandin F2α Receptor Antagonist on In Vitro Development of Bovine Embryos. Knoxville, TN: The University of Tennessee. Thesis. Bostian AW et al. Antimullerian Hormone Does Not Predict Outcome of Preimplantation Genetic Screening. Fertility and Sterility. 2014. 102(3): e63. Scientific Presentations Ward AM. Evaluating Effects of a Prostaglandin F2α Receptor Antagonist on In Vitro Development of Bovine Embryos. Graduate Seminar (Animal Science 596). The University of Tennesee – Knoxville. April 2007. Honors and Awards Recipient of Glenn G. Summers Scholarship 2007 Deans List 2002, 2003, 2004, 2005 Inducted into Gamma Sigma Delta Agricultural Honor Society 2007 Certified as Technical Supervisor by American Board of Bioanalysts 2010 Professional and Nonprofessional Societies 1 Secretary/Treasurer of Department of Animal Science Graduate Student Association 2006, 2007 Member of Gamma Sigma Delta Agricultural Honor Society 2007, 2008 Member of American Board of Bioanalysts 2010 - Present Member of American Society for Reproductive Medicine 2010 – Present Member of NAPSRx Professional Development Attended annual meeting of the International Embryo Transfer Society in Denver, CO. January 5 – 9, 2008. Attended 21st annual Texas Assisted Reproductive Technology Society Meeting in Austin, TX. August 1 2, 2009. Attended American Association of Bioanalysts’ College of Reproductive Biology Embryology & Andrology Review Course in Grapevine, TX. March 20 – 21, 2010. Attended annual meeting of American Society for Reproductive Medicine in Denver, CO. October 23 – 27, 2010. Attended annual meeting of American Society for Reproductive Medicine in San Diego, CA. October, 2013. Attended Southwestern Embryology Summit in Las Vegas, NV. January, 2014. Professional Training Animal Care and Use Beef Cattle Handling (NIAA), Animal Care and Handling Series Video Tape Beef Cattle Training, Level 1: Video module based on the FASS Ag Guide Dairy Cattle Training Level 1: Video module based on the FASS Ag Guide Introduction to the Ag Guide (FASS): Video tapes 1 & 2 Working with the University of Tennessee Knoxville IACUC Occupational Health and Laboratory Safety Biological Safety Level 2 Training sponsored by UT Biological Safety – Fall 2007 Occupational Health Program; Assessment provided by Amy Knowles – Fall 2006 Occupational Safety and Health Administration Training – 2009-Present Bloodborne Pathogen Training – 2009-Present Radiation Safety Radiation Certification – Fall 2007 Teaching Experience North Carolina State University – Spring 2014 Animal Science 221L – Reproductive Physiology and Development Lab Teaching Assistant for Dr. Scott Whisnant Prepared for and instructed student labs Helped write and graded weekly quizzes for students Helped prepare for and graded lab practicals for students North Carolina State University – Spring 2013 Animal Science 151L – Introduction to Animal Science Teaching Assistant for Dr. Jeannette Moore 2 Prepared for and instructed all student labs Wrote and graded weekly quizzes for students Wrote and graded lab practicals for students The University of Tennessee, Knoxville – Fall 2006 – Summer 2008 Mentoring Students Undergraduate Erin Bartley Courtney Crabtree Jessy Harris Graduate Erin Fitzgerald Estanislao Peixoto The University of Tennessee, Knoxville – Fall 2006 Animal Science 420 Advanced Reproduction Teaching Assistant for Dr. F. Neal Schrick Prepared for and planned student labs Conducted study sessions and graded exams North Carolina State University – Spring 2006 Animal Science 303 Principles of Equine Evaluation Teaching Assistant for Mr. Mike Yoder Preparation for and instruction of labs Listening to oral examinations North Carolina State University – Spring 2005 Animal Science 225 Principles of Animal Nutrition Teaching Assistant for Dr. Jeannette A. Moore Organized and formatted lecture notes for students Prepared for student examination review Graded exams Data Analysis Experience with SAS software, version 9.1 Experience with JMP Analysis of data generated from thesis work Statistics for Biological Research (Plant Science 561; Graduate). The University of Tennessee, Knoxville. Fall 2006 Design and Analysis of Biological Research (Animal Science 571; Graduate). The University of Tennessee, Knoxville. Spring 2007 Least Squares Analysis (Animal Science 572; Graduate). The University of Tennessee, Knoxville. Fall 2007 Advanced Applied Regression (Statistics 572; Graduate). The University of Tennessee, Knoxville. Spring 2008 Laboratory Experience MedTech For Solutions April 2015-Present Embryologist/Andrologist Perform all aspects of embryology, andrology and assisted reproductive technologies Atlantic Reproductive Medicine Specialists February 2015 – Present Per Diem Embryologist Lab Supervisor: Robin Arning 3 Carolina Conceptions January 2011 – January 2015 Lab Supervisor & Senior Embryologist Lab Director: Dr. Steve Voelkel, HCLD (ABB) Performance of Andrology and Embryology Procedures in a Human IVF Laboratory Use of aseptic technique Sterile preparation of culture medium Pipetting oocytes and embryos for manipulation Location of cumulus-oocycte complexes following retrievals Preparation of fresh and frozen semen samples for IVF and ICSI Washing sperm in preparation for intrauterine insemination Performing semen analyses Kreuger strict morphology analysis Cryopreservation of sperm Performance of ICSI Evaluation of in vitro produced embryos at all stages of development Performing assisted hatching Performing embryo transfers Cryopreserving embryos using vitrification Thawing embryos for frozen embryo transfer Trophectoderm Biopsy for PGD/PGS Maintenance of Embryo Culture Laboratory Cleaning and maintenance of CO2 and N2 incubators General embryo production laboratory cleaning and maintenance Immunology Maintenance and service of TOSOH immunology machine Running serum samples for various hormones associated with assisted reproductive technology Preparing blood and serum samples for analysis at off-site laboratory Office and Patient Care Duties Speaking with patients throughout the duration of their care at Carolina Conceptions Acting as donor sperm coordinator for the facility and patients Maintaining all records associated with the andrology laboratory and all laboratory certifications Presenting monthly and annual statistics associated with the andrology laboratory All paperwork associated with patients going through assisted reproductive technology Billing and maintaining inventory for sperm & embryo storage Austin IVF – August 2008 – January 2011 Embryologist Lab Director – Tom Turner, M.S. ELD (ABB) Performance of Andrology and Embryology Procedures in a Human IVF Laboratory Use of aseptic technique Sterile preparation of culture medium Pipetting oocytes and embryos for manipulation 4 Location of cumulus-oocycte complexes following retrievals Preparation of fresh and frozen semen samples for IVF and ICSI Washing sperm in preparation for intrauterine insemination Performing semen analyses and testing for anti-sperm antibodies Cryopreservation of sperm Performance of ICSI Evaluation of in vitro produced embryos at all stages of development Performing assisted hatching Performing embryo transfers Cryopreserving embryos using both slow-freeze and vitrification methods Thawing embryos for frozen embryo transfer Maintenance of Embryo Culture Laboratory Cleaning and maintenance of CO2 and N2 incubators General embryo production laboratory cleaning and maintenance Servicing and maintenance of pH meter Observed, but not Proficient In Biopsy for Preimplantation Genetic Diagnosis The University of Tennessee, Knoxville – August 2006 – August 2008 Graduate Research Assistant In Vitro Maturation, Fertilization and Culture of Bovine Embryos Use of aseptic technique Sterile preparation and handling of stocks and culture medium Mouth pipetting for oocyte/embryo manipulation Collection and evaluation of cumulus-oocyte complexes Preparation of frozen/thawed bovine sperm using Percoll purification Evaluation of in vitro produced embryos for cleavage stages and blastocyst development Fixation, mounting and Hoechst staining of bovine oocytes and embryos for nuclei counting, nuclear stage, and sperm penetration Maintenance of Embryo Culture Laboratory Cleaning and sterilization of glassware and instruments Cleaning and maintenance of CO2 and N2 incubators General embryo production laboratory cleaning and maintenance Servicing and maintenance of pH meter Sterilization of autoclaveable waste Observed, but not Proficient In Real Time Polymerase Chain Reaction Sperm morphology assessment Computer Software Experience Microsoft Office 2000 (Word, Excel and Power Point) Microsoft Office 2007 (Word, Excel and Power Point) SAS Version 9.1 5 JMP Endnote Metamorph Hamilton-Thorne-IVOS CASA References Available Upon Request 6