Download - Applied Science University

‫جامعة العلوم التطبيقية الخاصة‬
)‫ت‬.‫ع‬.‫( ج‬
APPLIED SCIENCE PRIVATE
UNIVERSITY
‫عمان – األردن‬
(A.S.U)
AMMAN - JORDAN
‫ف لن جعل م ن أردن الع رب جامعة لل عرب‬
Faculty: Pharmacy
Department : Clinical pharmacy and therapeutics
Academic Year: 2016 – 2017 First Semester
( Course
Syllabus )
Subject Name
Credit Hours
Course No.
Prerequisite
Practical Biochemistry
1 hour
0906213
0906212
Coordinator Name
Lecturer
Msc.Ghada A Amara
Dr .Nabeel Nuaimi
Room
No.
Concurrent course
E-mail
Office
Hours
[email protected]
[email protected]
Msc.Ghada A Amara
Course Objectives:
The experiments in this course intend to help students acquire basic working knowledge of biochemistry
espically the practical aspects of this study.
Course Description:
This course is intended for pharmacy students of the second year level along with biochemistry (1)
lectures and it consists of experiments consisting of basic skills and methods in biochemistry beside
experiments involving techniques of distingushing, identification and quantitation of biomolecules such
as amino acids, proteins, enzymes, crabohydrates and lipid.
Intended Learning Outcomes
After studying this course the student should be able to:
Successful completion of this course should lead to the following learning outcomes :
1- Students should be able to follow the saftey procedures and lab instruction.
2- Student should be able to identify and effeciently use the glasswares and equipments used in
the lab and differeniate between accuracy and percision.
3- Students should understand the concept of pKa values of acids and how to practically
determine its value.
4- Students should understand the concept of buffer solution and their preparation using the
Hendersson-Haselbach equation.
5- Student should be able to perform the different qualitative tests used for identification of
different amino acids.
6- Students should understand the concept of thin layer chromatography in seperation of
molecules and perform it in the seperation of a mixure of amino acids.
7- Studnets should perform and understand some of the technigues used in the seperation of
protiens such as salting out and isoelectric point precipitation, and study the different
factors that affect the structure of proteins.
1
8- Students should be able to perform an experiment to measure the activity of an enzyme and
study the effect of several factors on enzyme activity.
9- Students should perform tests to identify different carbohydrates including:
monosaccharides, disaccharides and polysaccharides.
10- Students should perform experiments on the chemical proporties of lipid such as solubility,
saponification and acid value.
Course Contents :
Week
1
2
Reference
(chapter)
Topic Details
- Saftey rules and general lab instructions.
- Accuracy and Precision of Laboratory Glassware
3
Measurement of pH of solutions and determination of the
pKa value of a weak acid
4
5
6
Preparation of Buffers
Qualitative tests for Identification of amino acids.
Chromatography: the use of Thin Layer Chromatography for
seperation of amino acids.
Isolation and Separation of Proteins
Denaturation of proteins
Factors Influencing Enzyme Activity
7
8
9
10
11
Topic Details
Practical
Biochemistry
mannual
Carbohydrates: identification tests for monosaccharies and
disaccharides.
Carbohydrates: identification tests for polysaccharides and
disaccharides
Lipids: Solubility of lipids, Saponification reaction of lipids
and acid value determination.
Final Lab Exam
12
13
14
15
Grade Distribution :
FINAL
EXAMINATION
Assessment
- Reports,
- quizzes
-Participation, conduct, lab work and assignments
- Final Examination
W EE K
Grade
15
25
10
Every lab
Six quizzes
Date
50
Practical
&theoretical
* Make-up exams will be offered for valid reasons. It may be different from regular exams in content and format.
Main Reference:
Last updated on
Manual of Biochemistry
26/ 9 / 2016 by : Ghada Abu Amara
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