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Biology Cell Structure and Function Cell Study Guide Vocabulary: Cell Cell Theory Nucleus Eukaryote Prokaryote Endoplasmic Reticulum Golgi Apparatus Lysosome Vacuole Cell Specialization Organelle Cytoplasm Nuclear Envelope Chromatin Chromosome Nucleolus Ribosome Facilitated Diffusion Phagocytosis Tissue Mitochondrion Chloroplast Cytoskeleton Centriole Cell Membrane Cell Wall Lipid Bilayer Active Transport Pinocytosis Organ Concentration Diffusion Equilibrium Osmosis Isotonic Hypertonic Hypotonic Endocytosis Exocytosis Organ System 7.1: Life is Cellular A. The Discovery of the Cell 1. Precipitated by the invention of the microscope a. 1665: Robert Hooke, cork i. Saw thousands of tiny, empty chambers ii. Called them cells, b/c they reminded him of monastery rooms b. Same time: Anton van Leewenhoek, pond water i. Saw tiny, living organisms 2. Cell Theory: a. All living things are composed of cells b. Cells are the basic units of structure and function in living things c. New cells are produced from existing cells B. Exploring the Cell 1. Light microscopes 2. Electron microscopes a. Capable of 1000x greater magnification b. Transmission EM i. Only dead samples ii. Interior structures and protein molecules c. Scanning EM i. Only dead samples ii. Exterior detail d. Scanning Probe microscopes i. Living samples ii. Single atoms can be viewed C. Prokaryotes and Eukaryotes 1. All cells have a cell membrane and all cells, at some point, have DNA 2. Prokaryote a. Small and simple b. Genetic material is not in a nucleus c. Ex. Bacteria 3. Eukaryote a. Larger and more complicated b. Genetic material is contained in a nucleus, separated from the rest of the cell Biology Cell Structure and Function Cell Study Guide 7.2: Eukaryotic Cell Structure A. Comparing the Cell to a Factory 1. Cells contain all sorts of different “employees”, with all kinds of specialized jobs 2. “Employees” are called organelles a. “Little organs” 3. Nucleus 4. Cytoplasm a. Portion of the cell outside the nucleus Biology Cell Structure and Function Cell Study Guide B. Organelles Organelle Location Structure Function Nucleus Nucleus Large Sack Contains DNA Nuclear Envelope Chromatin Around nucleus Nucleus Double layer membrane Granular DNA and protein Controls access to nucleus Forms chromosomes Chromosomes Nucleolus Nucleus Nucleus Dense, threadlike Small, dense area Allows for duplication of genetic material Begins ribosome assembly Cytoplasm Small particles of protein and RNA Protein Synthesis Cytoplasm Rough or Smooth (depending on whether ribosomes are attached) Lipid (smooth), protien (rough) and "export" assembly; Drug detoxification (smooth) Like a stack of pancakes Modify, sort, and packaged materials from the ER for storage inside the cell or secretion from the cell Small, enzyme filled sacks Breakdown of cellular "junk", digestion of large molecules into smaller, more useful ones Cytoplasm Sacks Storage, large central plant cell vacuole provides pressure to help support stems, etc. Cytoplasm Sack with the inner membrane folded up inside the outer Convert chemical energy into a useful form Cytoplasm Like mitochondria, except inner membranes are green due to chlorophyll Capture solar energy and convert it to chemical energy through photosynthesis Ribosomes Endoplasmic Reticulum Golgi Apparatus Lysosomes Vacuoles Mitochondria Chloroplast Cytoplasm Cytoplasm Cytoskeleton Cytoplasm A network of protein filaments Give shape, support and internal organization, aid in cell movement Microfilaments Cytoplasm Threadlike, made of actin Provide a tough, flexible framework Microtubules Cytoplasm Hollow, made of tubulin, arranged in a circle of 9 in an outer ring with 2 more in the center Maintain shape, form mitotic spindle to help separate chromosomes, build cilia and flagella Centrioles Cytoplasm Two pair of tubes, made from tubulin Organize cell division Biology Cell Structure and Function Cell Study Guide 7.3: Cell Boundaries A. Cell Membrane 1. Thin, flexible barrier composed of two layers of lipids 2. Regulates what crosses into and out of a cell 3. Provides protection and support 4. Dotted with embedded protein molecules that act as pumps or channels 5. Proteins often have carbohydrate chains attached, which act as identity cards so cells can easily recognize one another 5. “Fluid Mosaic” Model B. Cell Walls 1. Plants, algae, fungi, prokaryotes 2. Outside cell membrane 3. Porous to water, oxygen, CO2 and several other substances 4. Plant cell walls composed of the carbohydrate cellulose C. Diffusion Through Cell Boundaries 1. All cells live in a liquid environment 2. The cell membrane must regulate the movement of dissolved molecules 3. Concentration a. Mass of solute per volume of solution b. Ex. grams/Liters 4. Diffusion a. Particles tend to move from an area of high concentration to an area of low concentration b. Diffusion continues until the entire available volume is at roughly the same concentration i. Equilibrium ii. Particles still in motion, but there is no net change in concentration c. Substances that can cross a cell membrane will do so without using energy D. Osmosis 1. Diffusion of water through a semipermeable membrane 2. Since the membrane in permeable to water, but not to the solute, it is the water that moves from an area of high concentration to low until equilibrium is reached a. Seems backwards, because if water is concentrated, the solution is actually WEAK 3. Iso, hypo, and hypertonic a. Used to compare two solutions b. If they have the same concentration, they are isotonic c. If they are not, the more concentrated solutions is hypertonic while the less concentrated solution is hypotonic 4. Osmotic pressure a. Pressure exerted on the hypertonic side of a semipermeable membrane b. Cells are full of salts and other compounds i. Surrounded by fresh water, the water will move into the cell, possibly until the cell bursts ii. Surrounded by a more concentrated salt water solution, the water will move out of the cell, possibly until it shrivels and dies c. Animal cells are generally surrounded by isotonic fluids, like blood d. Plant cells store extra water in the central vacuole and depend on the cell wall to prevent bursting Biology Cell Structure and Function Cell Study Guide E. Facilitated Diffusion 1. Some molecules pass through membranes much more easily than they should 2. Protein channels act as carriers to allow large, strongly charged molecules easy access 3. Protein channels are specific to one and only one substance, but will allow that substance to pass freely in either direction 4. Still a diffusion process, so does not require energy F. Active Transport 1. Sometimes cells must move materials against a concentration difference 2. This requires energy 3. Transport proteins or protein pumps accomplish this 4. Can also be accomplished through endo and exocytosis a. Moves large molecules or solid clumps of material b. Endo- taking into the cell i. Cell membrane folds in and envelops whatever is being transported ii. Eventually pinches off and becomes a vacuole in the cell iii. Phagocytosis: solid particles iv. Pinocytosis: to take up liquid from the environment c. Exocytosis- pushing out of the cell i. Membrane of the vacuole containing substance fuses with cell membrane ii. Forces material out of the cell 7.4: The Diversity of Cellular Life A. Unicellular Organisms 1. Yeast 2. Algae 3. Bacteria B. Multicellular Organisms 1. Tend to engage in cell specialization a. Cells develop in different ways so that they can perform different tasks 2. Nerve cells, pancreatic cells, muscle cells, bone cells, etc… 3. In plants, guard cells regulate the exchange of water , carbon dioxide, oxygen etc. C. Levels of Organization 1. Cells 2. Tissues a. Made of a single type of cell b. Has a specific function c. Muscle, epithelial, nervous and connective tissue 3. Organs a. Made of many types of tissues working together to perform a complex task 4. Organ Systems a. Many organs working together to perform a specific function