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BI 200 - Exam #3A Spring 2001 Name Lab Section Disclaimer Consider each question, and answer each in the appropriate format (e.g., multiple choice). You may qualify your answer if you have reservations. If your comments have merit, you may receive partial or full credit. Multiple Choice - 1 point each 1. Which of the following is not involved in prokaryotic transcription? a. formyl-methionine b. sigma factor c. the promotor d. the operator region 2. The number of nucleotide bases on a strand of DNA required to code for a polypeptide consisting of 50 amino acids would be: a. 50 b. 150 c. 300 d. 450 e. 600 True or False (10 points) Place the whole word True or the whole word False before each of the following statements indicating their validity ___________ Transcription and translation are said to be coupled in prokaryotes. ___________ Eukaryotic genes contain non-coding regions called exons. 1 point each. Fill in the blank from the viruses listed on this page. There may be more than one correct answer, and you may use an answer more than once, but put only one answer for each. _________________________________ Any bacteriophage _________________________________ Any icosohedral virus _________________________________ Exhibits an unusually wide range of vertebrate hosts _________________________________ Your favorite virus _________________________________ Your least favorite virus _________________________________ Any helical virus _________________________________ Any enveloped virus _________________________________ Any complex virus _________________________________ HIV is this type of virus _________________________________ A temperate phage _________________________________ Causes a lytic infection in bacteria _________________________________ Causes a lytic infection in people _________________________________ Poliovirus is an example of this type of virus _________________________________ Causes a persistant infection _________________________________ Involved in phage conversion of C. diptheriae _________________________________ Causes small pox Mu Lambda T4 Beta M13 Orthomyxovirus Rhabdovirus Picornavirus Retrovirus Herpesvirus Rhinovirus Ebolavirus Papillomavirus Poxvirus 1 point each. Fill in the blank from the viruses listed on this page. There may be more than one correct answer, and you may use an answer more than once, but put only one answer for each. _________________________________ Any bacteriophage _________________________________ Any icosohedral virus _________________________________ Exhibits an unusually wide range of vertebrate hosts _________________________________ Your favorite virus _________________________________ Your least favorite virus _________________________________ Any helical virus _________________________________ Any enveloped virus _________________________________ Any complex virus _________________________________ HIV is this type of virus _________________________________ A virulent phage _________________________________ Causes a lysogenic infection in bacteria _________________________________ Causes a lytic infection in people _________________________________ Hepatitis A is an example of this type of virus _________________________________ Causes a latent infection _________________________________ Involved in phage conversion of C. diptheriae _________________________________ Causes chicken pox Mu Lambda T4 Beta M13 Orthomyxovirus Rhabdovirus Picornavirus Retrovirus Herpesvirus Rhinovirus Ebolavirus Papillomavirus Poxvirus Short answer (10 points). Discuss bacterial DNA in general, and the article “First Sequencing of Cell’s DNA Defines Basis of Life” specifically by addressing each of the following sub topics. a. What was the name of the organism studied? b. Where is it usually found, and what problems can it cause? c. What is the size in base pairs of the genome of the organism discussed in the paper? d. How many genes do that amount to? e. What fraction of those genes has been identified? f. How have they been recognized? g. What other organisms are being studied, and what cell-types do they represent? h. What is the shape of the cell discussed? i. Is the organism discussed competent? j. Name one of the scientists, and where they do their research. Diagram (10 points). Make a diagram of the coupled transcription and translation of the lac operon. Indicate the DNA, the lacZ, lacY, and lacA genes, RNA polymerase, polycistronic mRNA, ribosomes, and newly formed proteins. Also indicate the promoter, operator, where the RNA polymerase binds and where the repressor binds. This is also known as the “Christmas tree” structure, or polysome.
