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Download Lab 2- Bacterial cell structures/simple staining
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Lab Two Bacterial Cell Structures Bacterial Structures and Shapes Simple Staining procedures Part One - Bacterial Structures and Shapes In this exercise, you will view prepared slides of bacteria that make capsules, spores/endospores, or have unique shapes/structures beyond the standard rods or cocci you have seen so far. Review lecture notes, Chapter 3 about all these microbes and structures. Prepared Slides to View - Work In Pairs Bacterial Capsules, 100X objective Flagella Fat/PHB bodies Bacillus stained for endospores, 100X objective Treponema or Spirochetes, unusual shape and structure, 100X objective Clostridium Staphylococcus Anabaena (view at 40X) make 2 different kinds of cells - a smaller green one that is photosynthetic and a larger colorless one that fixes nitrogen. Note and include BOTH cell types in your final drawing. Part Two - Slide preparation of Microbes Procedures Each person stains, Streptococcus, Serratia, and Yogurt - label slides as such Mix 1 loopful of water with a loop of each bacteria and set slides aside to COMLETELY air-dry Show your instructor your drying slides to confirm you have enough material on them After you have finished ALL other exercises, pass dried slides through flame 3 times to heat-fix Biology 318, Lab Two, 1 Biology 318 Worksheet Due Next Lab - Turn in Individually Name: (1) 12 pts. Prepared Slides: Bacterial Structures and Shapes. 100X Objective Capsule (a) Where located in cell: (b) What is it made of? (c) Function(s): DRAW AN ARROW TO THE PART FEATURED!!! Endospores (a) Define this term: (b) Function(s): (c) Example microbe that uses: DRAW AN ARROW TO THE PART FEATURED!!! Treponema (a) What is this shape: (b) Name a disease caused by this organism: Flagella Identify the two types Biology 318, Lab Two, 2 Fat/PHB bodies (a) Function: (b) Example microbe that uses: DRAW AN ARROW TO THE PART FEATURED!!! Clostridium (a) What is this shape: (b) Name a disease caused by this organism: Staphylococcus (a) What is this shape: (b) Name a disease caused by this organism: Anabaena a) Draw and label the two cell types, b) Identify the functions of each: Biology 318, Lab Two, 3 (2) 6 pts. Stain Results 100X Objective Serratia Streptococcus Yogurt Biology 318, Lab Two, 4
