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台中班 2012 暑期生化小考
脂質&核酸
單選(2%)
1.下列何種是一種 ω3 脂肪酸？
(A) Oleic acid
(C) α-Linolenic acid
(B)Linoleic acid
(D) γ-Linolenic acid.
2.下列何者是 Secondary bile acid
(A) Cholic acid
(B) Glycocholic acid
(C) Deoxycholic acid
(D) Glyco-Chenodeoxycholic
acid.
3.下列那一個脂蛋白(Lipoprotein)所攜帶的三酸甘油酯(Triacylglycerol)最多？
(A) VLDL
(B) IDL
(C) LDL
(D) HDL
4.下列那一個 Apolipoprotein 是 Lecithin：cholesterol Acyltransferase 的 Activator？
(A) Apo A-I (B) Apo A-II
(C) Apo C-II (D) Apo E
5. 下列那些脂蛋白(lipoprotein)含有 apo-B100？
(A) chylomicron
(B) VLDL
(C) LDL
(D) HDL.
6.可以與 LDL-receptor 結合而將脂蛋白(Lipoprotein)帶入細胞的是
(A) Apo A-II
(B) Apo B-48
(C) Apo C-II
(D)Apo E
7. Which enzyme(s) involved in the free fatty acid β-oxidation？
(A) thiolase
(B) thiokinase
(C) HMG-CoA lyase
(D) carnitine acyltransferase I
Phospholipase C
8. Oleoyl-CoA
9acetyl-CoA + X ATP，請問 X=？
β-oxidation
(A)38 (B)40 (C)146 (D)148
9. Ketone bodies 主要是在下列那一個器官或組織中產生？
(A) Brain
(B) Heart
(C) Liver
(D) Muscle
(E) Kidney
10.下列敘述何者正確：
(A)Insulin 會促進(activate)Hormone sensitive lipase 活性
(B)Insulin 會促進 β-oxidation 反應
(C)Insulin 會促進 Acetyl-CoA carboxylase 活性
(D)Insulin 會促進 Ketone bodies 的產生
11. The most importance source of reducing equivalent for fatty acid synthesis in the liver is
(A) Cori cycle
(B) lipolysis
(C) glycolysis
(D) the pentose phosphate pathway
(E) the
(E)
tricarboxylic acid cycle.
12.Stearic acid 在人內可轉變成下列那些脂肪酸？
(A) oleic acid
(B) linoleic acid
(C) linolenic acid
(D) arachidonic acid
13.必需脂肪酸(essential fatty acids)缺乏時血漿或細胞中那一個脂肪酸會有堆積(accumulation)的現象
(A) C20：3 ω-9
(B) C20：4 ω-6
(C) C20：4 ω-3
(D) C20：5 ω-3
14. 下列何者在膽固醇合成的過程中的先後次序是正確的？
(A)Acetyl-CoA → Mevalonate → HMG-CoA → Squalene → Cholesterol
(B)Acetyl-CoA → Gernesyl diphosphate → HMG-CoA → Squalene → Cholesterol
(C)Acetyl-CoA → HMG-CoA → Squalene → Gernesyl diphosphate → Cholesterol
(D)Acetyl-CoA → HMG-CoA → Farnesyl diphosphate → Squalene → Cholesterol
15. 下列那一個酵素是膽固醇(cholesterol)合成過程中的關鍵酵素(key enzyme)，可以被膽固醇及
Mevastatin 抑制
(A) HMG-CoA Lyase
(B) HMG-CoA Reductase
(C) Mevalonate kinase
(D) Squalene synthase.
16.在細胞中可將膽固醇(Cholesterol)轉變成膽固醇酯(Cholesteryl ester)的酵素是
(A)Acyl-CoA：cholesterol acyltransferase
(C)Lecithin：cholesterol acyltransferase
(B)Cholesteryl ester transfer protein
(D)HMG-CoA reductase
17. Arachidonic acid 經 cyclooxygenase 作用可產生
(A) TXA1
(B) PGE2
(C) PGE3
(D) LTA3
18. Which statement about Na+, K+-ATPase correct?
(A)It pumps out 2 Na+ and pumps in 3 K+ per molecule of ATP
(B)It produces a positive transmembrane potential of 50 to 70 mV
(C)It contains two subunits each of which spans the membrane
(D)It can be activated by ouabain and digitalis
(E)all the above are correct
19. Decreases fluidity of a biological membrane.
(A) Increased temperature
(B) Increased number of cis double bonds in fatty acyl chains
(C) Increased content of short chain fatty acyl group
(D) Increased content of cholesterol
20.下列何者不是 nucleoside 之組成？
(A) adenosine
(B) guanosine
(C) cytosine
(D) uridine
21. AMP(adenosine monophosphate)是一種：
(A) ribobase
(B) ribonucleotide
(C) ribonucleoside
(D) ribonucleic acid
22.人體內之 DNA 大部分以那一種形式存在：
(A) A form
(B) B form
(C) H form
(D) Z form
23.下列何種 DNA 具最低的 melting temperature(Tm)？
(A)60%GC 及 40%AT
(B) 50%GC 及 50%AT
(C)80%GC 及 20%AT
(D)20%GC 及
80%AT
24.大腸桿菌的一段雙股 DNA，其 G+C 比 A+T 之含量大 16％。試問其 T 的含量百分比為多少？
(A) 27
(B) 24
(C) 21
(D) 18
25.如果一病毒基因體之鹼基組成分析結果為 A=24%, G=24%, T=26%, C=26%, 此病毒最可能含有
(A) Double-stranded RNA
Single-stranded DNA
(B) Double-stranded DNA
(C) Single-stranded RNA
(D)
(E) A DNA-RNA hybrid
26.人類單套體(haploid)之染色體 DNA，以 B form 方式存在之實際長度(contour length)大約有多長？
(A) 0.1 公分
(B) 1 公分
(C) 10 公分
(D) 1 米
27. 類基因體由多少個鹼基對(base pairs)所組成：
(A) 4×106
(B) 6×107
(C) 4×108
(D) 6×109
28. RNA 5’-pApCpGpUpCOH-3’經鹼水解後, 會出現下列那一種產物
(A) pAp
(B) pCp
(C) pGp
(D) pUp
(E)pCOH
29.大腸桿菌在複製 DNA 時，下列那一敘述是對的？
(A)複製是由特定一個啟始點向兩方向合成
(B)複製是一種 conservative replication
(C)複製時主要的合成酵素為 DNA polymerase I
(D)複製時是由一小段 DNA 當引子(primer)進行 DNA 合成
30.在大腸桿菌(E. coli)之 DNA 複製過程中，主要是以下列那一種聚合酵素進行 DNA 合成：
(A) DNA polymerase I
(B)DNA polymerase II
(C) DNA polymerase III
(D) Klenow
fragment
31.下列有關 E. coli DNA 複製的敘述，何者為非
(A) DNA 複製的聚合反應皆以 5’→3’為唯一方向
製時的主要酵素是 DNA polymerase III
(B) DNA 複製的開始須要 RNA 的參與
(C)複
(D) DNA 複製的起始點是與序列無關的隨機事件
32. Which of the following statements about DNA polymerase III holoenzyme from E. coli are correct ?
(A) It elongates a growing DNA chain approximately 100 times faster than dose DNA polymerase I.
(B) It associates with the parental template, adds a few nucleotides to the growing chain, and then dissociates
prior to initiating another synthesis cycle.
(C) It maintains a high fidelity of replication, in part by acting in conjunction with a subunit containing a
3'→5' exonuclease activity.
(D) When replicating DNA, it is a molecular assembly composed of at least 8 different kinds of subunits.
33. Mismatched repair 所用之 polymerase 為
(A) DNA polymerase I
polymerase II
(B) DNA polymerase II
(C) DNA polymerase III
(D) RNA
(E) RNase H
34. Place the following events in the order in which they would occur during general recombination between
two DNA molecules.
(A) Strand exchange occurs between duplexes via branch migration at the crossover point.
(B) A pair of strands with similar sequences is cleaved in each duplex.
(C) Two duplexes align at a region of sequence similarity.
(D) Each invading strand becomes covalently joined to its corresponding strand in the other duplex.
(E) Strands of the recombinant intermediate are cleaved at or near the crossover point and are joined to-their
corresponding strands in each duplex.
(F) The end of each single strand invades the other duplex and forms base pairs with its complementary
strand.
35.The sigma factor () of prokaryotic RNA polymerase is part of the core enzyme. Which of the following
statement is NOT TRUE for sigma factor..
(A)It is released from the core enzyme after the first phosphodiester bond has been formed.
(B)It is inhibited by -amanitin.
(C)It must be present for transcription to occur.
(D) It specifically recognize the promoter sites.
36. E. coli 進行 RNA 生合成時 RNA polymerase 中的下列那一個 subunit 具有該酵素催化作用？
(A) 
(B) 
(C) 
(D) 
37. Which one of the following statement is INCORRECT in describing prokaryotic RNA transcription？
(A)There is no processing reaction for all RNA transcripts.
(B)RNA polymerase is responsible for all mRNA, tRNA and rRNA transcriptions.
(C)mRNA can be polycistronic.
(D)RNA transcription can be coupled with protein translation.
38. Which one of the following RNA contains a 7-methylguanosine triphosphate cap and a polyadenylate
segment
(A) mRNA
(B) rRNA
(C) tRNA
(D) hnRNA
39.下列那一個化合物為最佳之 ribozyme 可進行 splicing？
(A) AMP
(B) GMP
(C) CMP
(D) TMP
39.Which one of the following RNA has the highest percentage of modified bases？
(A) mRNA
(B) rRNA
(C) tRNA
(D) hnRNA
40. The enzyme which can synthesize complementary DNA(cDNA) from mRNA is
(A) Klenow enzyme
(B) RNA polymerase II
(C) Reverse transcriptase
(D) DNA polymerase I
41.下列何種酵素對-amanitin 毒素的抑制作用最敏感？
(A) DNA polymerase
(B) RNA polymerase I
(C) RNA polymerase II
(D) RNA polymerase III
42.下列那一種抗生素可抑制細菌 RNA polymerase 之活性？
(A) Erythromycin
(B) Rifampicin
(C) Streptomycin
(D) Chloroamphenicol
43.下列那一種胺基酸之密碼(codon)種類最少？
(A) Asparagine
(B) Histidine
(C) Tryptophan
(D) Tyrosine
44.在人類基因之遺傳密碼中，何者不是終止密碼：
(A) UAA
(B) UGG
(C) UAG
(D) UGA
45. Degeneracy of the genetic code denotes the existence of：
(A)multiple codons for a single amino acid
coding for different amino acids
(B)codon consisting of 2 bases
(C)a given codon
(D)codons that include one or more of the “unusual” bases
46. Which of the following RNA has the highest percentage of modified bases
(A) mRNA
(B) rRNA
(C) tRNA
(D) hnRNA
47.當一 mRNA 進行轉譯時，5’-UUC-3’位在 A site 時會和具下列何種 anticodon 序列的 aminoacyl-tRNA
結合：
(A) 5’-AAG-3’
(B) 5’-CUI-3’
(C) 5’-IAA-3’
(D)5’-AAI-3’
48. Which statement is NOT TRUE regarding the accuracy of protein synthesis？
(A)The ribosome contains an exopeptidase for proofreading.
(B)Aminoacyl tRNA synthetases hydrolyze incorrect amino acyl-tRNAaa.
(C)The error rate in protein synthesis is about 1 in 10,000.
(D)tRNA binding to amino acyl tRNA synthetase is highly specific.
49. The antibiotic puromycin can directly inhibit
(A)DNA replication by binding to ori site
(B)RNA transcription by binding to σ factor
(C)Protein translocation by binding to the P site of ribosome
(D)Protein translocation by binding to the A site of ribosome
50. Coactivating protein influences eukaryotic gene expression by
(A) binding to TATA box.
(B) interacting with RNA polymerase directly to enhance transcription.
(C) interacting with activators.
(D) binding to cis-element in the promoter region.
台中班 2012 暑期生化小考
脂質&核酸
單選(2%)
1.(C)
2.(D)
3.(A)
4.(A)
5.(B)(C)
6.(D)
7.(A)(D)
8.(A)
9.(C)
10.(C)
11.(D)
12.(A)
13.(A)
14. (D)
15.(B)
16.(A)
17.(B)
18. (B)
19.(D)
20.(C)
注意：
39 題有兩題
5、7、32、35 是複選題
第 34 題不是選擇題是排序題
21.(B)
22.(B)
23.(D)
24.(C)
25.(D)
26.(D)
27.(D)
28. (A)
29.(A)
30.(C)
31.(D)
32.(C)(D)
33. (C)
34. (C)(B)(F)(D)(A)(E)
35.(A)(B)
36. (B)
37. (A)
38. (A)
39. (B)
39. (C)
40. (C)
41.(C)
42.(B)
43.(C)
44.(B)
45. (A)
46. (C)
47.(C)
48. (A)
49. (D)
50. (C)