Download MAR - OSU Biochemistry and Molecular Biology

BIOC 3653: Survey of Biochemistry
Spring 2011
Instructor:
Dr. Ben Sandler
348G Noble Research Center
405-744-6194
[email protected]
Chief Teaching Assistant:
Steven Pennington
[email protected]
Teaching Interns:
John Paul Long
Jon Downs
Lecture:
[email protected]
[email protected]
MWF 12:30-1:20PM, Animal Science 123
Office Hours:
Ben Sandler: TR 9:00-10:20, 348G NRC, or by appointment.
Review sessions TBA.
Text: Biochemistry, 6th ed., Berg, Tymoczko, and Stryer. (Required.)
“Skeleton Key” study guides, (by Dr. Sandler, free on the D2L site. Required.)
The Secret Life of Equations, (by Dr. Sandler, free on the D2L site. Recommended.)
Prerequisites:
You are expected to have a working knowledge of algebra, general chemistry, and
organic chemistry. You will need to be able to use logarithms, exponents, and scientific
notation; to rearrange algebraic equations; to balance chemical equations; to understand
the relationships between atomic number, valence, atomic structure, and reactivity; to
calculate pH and use equilibrium constants and ionization constants; to know the
properties (such as reactivity and polarity) of the major chemical functional groups; to
know how to calculate concentration, among other skills. If you feel like you’re rusty on
any of these, you must refresh your memory of these topics before the first exam.
Note: you won’t be allowed to use a calculator on exams. Make sure you know how to
calculate simple logarithms (such as log 0.01=?) with pencil and paper. I’ll put a
logarithms study guide on the D2L site, in case your logarithm skills are rusty.
D2L website:
https://oc.okstate.edu
The D2L site is an essential part of this class. It is your responsibility to check this site
regularly for announcements, homeworks, grades, etc. The Powerpoint files for lectures
will also be posted here. Keep your eye on the site as additional resources are added!
Lecture Videos:
Videos of the lectures will be available through the course website. (Instructions on how
to view these videos will also be posted.)
Homework:
Homework problems will be regularly assigned and posted on the D2L website. As
always, write clearly- unintelligible responses will receive a zero. These homeworks will
be spot-graded (i.e., we will pick a subset of questions to grade on each homework,) and
you will also receive some points for completing all the questions, even if your answers
are incorrect.
You are encouraged to work collaboratively on the homework. But, do not copy other
people’s homework- even when you work together, your final answer must be your own,
written in your own words.
In order to be considered complete, answers to homework questions must show all your
work, complete with your reasoning and calculations, and be presented in a neat and
organized fashion. Remember, the goal is not merely to learn the material, but also to
learn how to communicate your knowledge effectively.
Exams:
There will be four midterm exams, 50 minutes each, during the semester. You will need
to bring your own orange Scantron sheet to each exam. Be sure to write clearlyunintelligible responses will get a zero!
The lowest score on a midterm will be dropped. The final exam will be 2 hours long, and
will cover information from the entire course.
Makeup exams:
There are no makeup exams in this class. Instead, if you miss an exam, it will be given
the same percentage grade as your final exam grade.
Grading Scheme:
Your grade will be based on your performance on the homework (4 homeworks, at 20
points each,) the best 3 out of 4 midterms (160 points each,) and the final exam (240
points.) The total number of points possible is 800 points. (This figure does not include
bonus points.) The assessment quiz does not count toward your grade.
You must earn at least 720 points to make an A,
at least 640 points to make a B,
at least 560 points to make a C, and
at least 480 points to make a D.
Academic dishonesty and misconduct:
Students are, as always, expected to follow the rules. Academic dishonesty and
misconduct are defined in the OSU Policy and Procedures letter 2-0822. If you are found
to have engaged in dishonesty or misconduct, the punishment will be at minimum an F on
an assignment, up to and including an F in the course or worse.
All exams are closed-book, closed-notes. Use of any notes, either on paper or on a
calculator, is not permitted and will be considered cheating, and dealt with accordingly.
Use of a calculator on an exam will also be considered cheating.
Attendance:
Attendance is strongly encouraged, but regular attendance is not required. For paperwork
purposes, we will be taking roll every day starting on the second Wednesday, until
roughly the midpoint of the semester. Only sign your own name!
Disabilities:
If you have special needs, please contact Dr. Sandler as soon as possible so that we can
work with you and the Office of Disabled Student Services (326 Student Union) to
provide reasonable accommodations for you.
What to Study:
You are only required to read the sections of the textbook that are covered in lecture.
Exam questions will be taken from the lectures and Skeleton Keys, not from the textbook.
How to Make an A in This Class:
Biochemistry is very different from many of the subjects you’ve studied before. Before
you could even begin to study biochemistry, you first had to study a great deal of biology
and chemistry- particularly organic chemistry. Now, you’re going to need to combine
your knowledge of biology and chemistry in order to understand biochemistry.
Make sure you remember what you learned in your biology, general chemistry, and
organic chemistry courses. I’ll give you an assessment quiz, so that you can figure out
what topics you need to review. I’ll also post some review guides on the D2L site.
Remember, too, that biochemistry is about narrative. For example, when your body
breaks down foodstuffs, it does so in a series of steps. Understanding the biochemistry of
metabolism means understanding what events happen, in what sequence, and why. Part
of understanding that means understanding the basic principles involved, and being able
to spot those concepts in different contexts. A good rule is this: if I mention a concept
twice in class, in different contexts, then it will definitely be on the exam.
Remember: merely being able to “plug and chug” an equation or memorize a graph will
not enable you to do well in this class! It is essential that you genuinely understand what
the material means. Many homework problems will require you to problem-solve, and
that may include recognizing what extra information you need to have in order to solve
the problem, and finding it online.
And, most important of all, remember that the professor and teaching assistants are here
to help you. If you’re finding biochemistry to be a challenging subject- and most people
do- don’t hesitate to come to our office hours. We are genuinely eager to help you!
Extra credit homework
Two extra credit homeworks, each worth five points each, will be assigned during the
semester. These will not be graded unless you are within 10 points of a grade cutoff.
Student learning outcomes for BIOC3653
1. Students, given the Henderson-Hasselbalch equation, will able to perform pH
calculations, predict the charge and protonation state of biomolecules in physiological
solution, and describe their behavior and function in terms of their state.
2. Students will master the basic principles of protein structure. They will be able to
recall, identify, and sketch all 20 amino acids, and classify them according to their
properties, including the special properties of glycine, alanine, proline, and cysteine.
They will be able to recall, define, and identify primary, secondary, tertiary, and
quaternary structure. They will be able to recognize the alpha helix and beta sheet in
representations of backbone traces and ribbon diagrams.
3. Students will master the basic principles of nucleic acid structure and function. They
will be able to recall and identify the differences between purines and pyrimidines, and
between bases, nucleosides, and nucleotides. They will be able to recall, list and describe
the differences between DNA and RNA, and explain the ways these differences affect the
function and behavior of nucleic acids.
4. Students will be able to produce a written explanation of how biological systems use
randomness and selection to produce new information. They will be able to recognize
features of biochemical systems as products of divergent or convergent evolution.
5. Students will master the principles of Gibbs Free Energy. Students will be able to
make both quantitative and qualitative predictions of the outcome of a reaction, or of the
distribution of conformational states of an enzyme, given either qualitative or quantitative
information about the thermodynamics of the system. This includes being able to
perform calculations involving ∆G, ∆Go, and ∆Eo, once given the equations.
6. Students will be able to determine and explain, in writing, where the energy comes
from to drive enzymatic reactions or changes in protein conformation, using the “Ladder
of Reactivity” and other chemical principles.
7. Students will be able to recall and explain in writing the significance of kcat, Vmax,
and Km for enzyme kinetics. They will be able to recall and explain in writing the
mechanisms of inhibition of competitive, uncompetitive, and noncompetitive inhibitors,
and how these mechanisms result in their effects on the kinetic parameters.
8. Students will be able to recall and describe in writing, using standard conventions, the
structures and roles of carbohydrates, lipids, and membranes in cells. They will be able
to describe, in writing, how differences in the structures of these molecules result in
differences in their properties and roles.
9. Students will be able to solve written problems using the core principles of metabolism
and bioenergetics, in terms of reduction potentials.
10. Students will be able to recall, recognize, list, and explain important features held in
common by a range of signal transduction pathways.
11. Students will be able to recall and explain in writing how carbohydrates, lipids, and
proteins are broken down by the body, how energy is extracted from them, and how that
energy is stored as ATP.
12. Students will be able to recall, list, and explain in writing the differences between
lipid synthesis and breakdown.
13. Students will be able to recall and define in writing a number of important
biochemical terms.
Tentative Lecture, Quiz, and Exam Schedule, Spring 2011
DATE
JAN.
Mon. 10
Wed. 12
Fri. 14
Mon. 17
Wed. 19
Fri. 21
Mon. 24
Wed. 28
Fri. 28
Mon. 31
FEB
Wed. 2
Fri. 4
Mon. 7
Wed. 9
Fri. 11
Mon. 14
Wed. 16
Fri. 18
Mon. 21
Wed. 23
Fri. 25
Mon. 28
MAR
Wed. 2
Fri. 4
Mon. 7
Wed. 9
Fri.11
14-18
Mon. 21
Wed. 23
Fri. 25
Mon. 28
Wed. 30
APRIL
Fri 1
Mon. 4
Wed. 6
Fri. 8
Mon. 11
Wed. 13
Fri. 15
Mon. 18
Wed. 20
Fri. 22
25-29
Mon. May 2
Topic
Chapter
Introduction. Assessment Quiz.
Chemistry review. Protein composition and structure
Protein composition and structure
NO CLASS- MLK DAY
Exploring proteins and proteomes
DNA, RNA, and the flow of genetic information
Exploring genes and genomes
Exploring evolution and bioinformatics
Hemoglobin: Portrait of a protein in action
Exam 1 (Chapters 1-6)
1
2
2
Enzymes: Basic concepts and kinetics
Enzymes: Basic concepts and kinetics
Enzymes: Basic concepts and kinetics
Catalytic strategies
Regulatory strategies
Carbohydrates
Lipids and cell membranes
Signal-transduction pathways
Metabolism: Basic concepts and design
Metabolism: Basic concepts and design
Glycolysis and gluconeogenesis
Exam 2 (Chapters 7-12, 14)
8
8
8
9
10
11
12
14
15, & pg 502-508
15, & pg 502-508
16
Glycolysis and gluconeogenesis
Glycolysis and gluconeogenesis
The citric acid cycle
The citric acid cycle
The citric acid cycle
NO CLASS- SPRING BREAK
Oxidative phosphorylation
Oxidative phosphorylation
Oxidative phosphorylation
The light reactions of photosynthesis
The light reactions of photosynthesis
16
16
17
17
17
Glycogen metabolism
Exam 3 (Chapters 15-18)
Glycogen metabolism
Fatty acid metabolism
Fatty acid metabolism
Fatty acid metabolism
Protein turnover and amino acid catabolism
Protein turnover and amino acid catabolism
Review of metabolism
Exam 4(Chapters 19, 21-23)
Dead week: Review
FINAL EXAM 10:00-11:50 AM
21
3
4
5
6
7
18
18
18
19
19
21
22
22
22
23
23
HW
HW 1
HW2
HW3
HW4