Separation Technique for the Determination of Highly Polar
... spectrometry [10,11]. MS and NMR are among the most important emerging technologies in metabolomics, offering the shortest route toward metabolite identification and quantification [12]. NMR has demonstrated great potential, essentially due to the high measurement reproducibility and the high throug ...
... spectrometry [10,11]. MS and NMR are among the most important emerging technologies in metabolomics, offering the shortest route toward metabolite identification and quantification [12]. NMR has demonstrated great potential, essentially due to the high measurement reproducibility and the high throug ...
Gel Electrophoresis
... achieved by applying a higher field strength or a longer pulse time in the separation direction. The advantage of this method is the simple design. The disadvantage is the long separation time, because the molecules migrate backwards for part of the time. A wide range of sizes of DNA molecules can be ...
... achieved by applying a higher field strength or a longer pulse time in the separation direction. The advantage of this method is the simple design. The disadvantage is the long separation time, because the molecules migrate backwards for part of the time. A wide range of sizes of DNA molecules can be ...
Supplementary materials Method 1: liquid chromatography for
... column (2.5 × 30 cm). The enzyme was then eluted with buffer A (flow rate, 90 ml/h). Ammonium sulfate was added to the pooled active fractions to a final 2-M concentration. The enzyme solution was then applied to a Toyoperal Phenyl-650M column (2.5 × 15 cm) pre-equilibrated with buffer A containing ...
... column (2.5 × 30 cm). The enzyme was then eluted with buffer A (flow rate, 90 ml/h). Ammonium sulfate was added to the pooled active fractions to a final 2-M concentration. The enzyme solution was then applied to a Toyoperal Phenyl-650M column (2.5 × 15 cm) pre-equilibrated with buffer A containing ...
BioTeke Corporation
... step to remove cellular metabolite and proteins etc. Finally use low salt elution to elute purified genome DNA from silica membrane. ...
... step to remove cellular metabolite and proteins etc. Finally use low salt elution to elute purified genome DNA from silica membrane. ...
Fundamentals and - 17th International Symposium on Chiral
... enantioseparations via HPLC. The course begins with an overview of linear and nonlinear chromatography. Typical phenomena commonly observed when high-concentrated samples are injected in chromatographic columns will be described in relation to the thermodynamics (namely, adsorption isotherms) and th ...
... enantioseparations via HPLC. The course begins with an overview of linear and nonlinear chromatography. Typical phenomena commonly observed when high-concentrated samples are injected in chromatographic columns will be described in relation to the thermodynamics (namely, adsorption isotherms) and th ...
Electrophoretic_techniques2003
... The sample buffer contain ionisable tracking dye,usually bromophenol blue, that allows the electrophoretic run to be monitored. ...
... The sample buffer contain ionisable tracking dye,usually bromophenol blue, that allows the electrophoretic run to be monitored. ...
PicodropTM
... contamination or carry-over on the sample platform. The advanced polymer technology used to make the UVpette tips ensures precise UV absorbtion measurements between 220nm and 950nm, making it ideal for applications involving nucleic acid, protein or microarray dye concentration determination. ...
... contamination or carry-over on the sample platform. The advanced polymer technology used to make the UVpette tips ensures precise UV absorbtion measurements between 220nm and 950nm, making it ideal for applications involving nucleic acid, protein or microarray dye concentration determination. ...
ion exchange chromatography
... Ion Exchange Chromatography Ion exchange chromatography -- is a separation based on charge Used for almost any kind of charged molecules --- large proteins, small nucleotides and amino acids Ion-exchange chromatography preserves analyte molecules on the column based on ionic interactions Mobile ...
... Ion Exchange Chromatography Ion exchange chromatography -- is a separation based on charge Used for almost any kind of charged molecules --- large proteins, small nucleotides and amino acids Ion-exchange chromatography preserves analyte molecules on the column based on ionic interactions Mobile ...
Pulsed Field Gel Electrophoresis - Bio-Rad
... The voltage gradient describes the strength of the electrical field and is represented as V/cm, where the total voltage is divided over the distance between two electrodes. Since that distance in a CHEF gel box is approximately 33 cm, a 200 V run is approximately 6 V/cm. Most CHEF protocols are opti ...
... The voltage gradient describes the strength of the electrical field and is represented as V/cm, where the total voltage is divided over the distance between two electrodes. Since that distance in a CHEF gel box is approximately 33 cm, a 200 V run is approximately 6 V/cm. Most CHEF protocols are opti ...
Dr Asmat Salim MM707-electrophoresis 2014
... In the biological system, many molecules are electrically charged and will move if electric field is applied. In electrophoresis, macromolecules are characterized by their rate of movement in an electric field. This technique is used to (1) distinguish molecules on the basis of charge and shape ...
... In the biological system, many molecules are electrically charged and will move if electric field is applied. In electrophoresis, macromolecules are characterized by their rate of movement in an electric field. This technique is used to (1) distinguish molecules on the basis of charge and shape ...
Presentazione standard di PowerPoint
... • High Q microcavities, with strong spatial localization of the field, well respond to this principle and receive an even greater interest in many fundamental processes in photonics (e.g.: QED & NL processes; biosensing….) ...
... • High Q microcavities, with strong spatial localization of the field, well respond to this principle and receive an even greater interest in many fundamental processes in photonics (e.g.: QED & NL processes; biosensing….) ...
SafeView - NBS Biologicals
... This dye replaces Ethidium Bromide (toxic, potential mutagen) for visualisation of DNA or RNA in Agarose gel. SafeView is noncarcinogenic and causes significantly fewer mutations in the Ames-test and tests negative in both the mouse marrow chromophilous erythrocyte micronucleus test and mouse sperma ...
... This dye replaces Ethidium Bromide (toxic, potential mutagen) for visualisation of DNA or RNA in Agarose gel. SafeView is noncarcinogenic and causes significantly fewer mutations in the Ames-test and tests negative in both the mouse marrow chromophilous erythrocyte micronucleus test and mouse sperma ...
Capillary electrophoresis tandem mass spectrometry of bromine
... The coupling of mass spectrometer (MS) with liquid-phase separation systems has great potential because it provides efficient separation and selective mass identification. Capillary electrophoresis (CE) is a fast, highly efficient separation method for small sample quantities; therefore, interfacing ...
... The coupling of mass spectrometer (MS) with liquid-phase separation systems has great potential because it provides efficient separation and selective mass identification. Capillary electrophoresis (CE) is a fast, highly efficient separation method for small sample quantities; therefore, interfacing ...
الشريحة 1
... separating molecules based on their charge and or size. This technique is simple: Biological samples are prepared and placed in gel (matrix). Electricity is then run through the matrix, causing molecules in the samples to separate. ...
... separating molecules based on their charge and or size. This technique is simple: Biological samples are prepared and placed in gel (matrix). Electricity is then run through the matrix, causing molecules in the samples to separate. ...
JSB-302
... The JSB-302 is designed for nucleic acid screening electrophoresis. The maximum suggested applied voltage for the electrophoresis of DNA in agarose gels using the JSB-302 is 150 volts. In a 1% TBE gel, this translates into a run time of approximately 1 hour. Lower voltages may be used, of course, an ...
... The JSB-302 is designed for nucleic acid screening electrophoresis. The maximum suggested applied voltage for the electrophoresis of DNA in agarose gels using the JSB-302 is 150 volts. In a 1% TBE gel, this translates into a run time of approximately 1 hour. Lower voltages may be used, of course, an ...
Ion exchange chromatography
... An ion exchanger consists of an insoluble matrix to which charged groups have been covalently bound. The charged groups are associated with mobile counter-ions. These counter-ions can be reversibly exchanged with other ions of the same charge without altering the matrix. 1-Positively charged excha ...
... An ion exchanger consists of an insoluble matrix to which charged groups have been covalently bound. The charged groups are associated with mobile counter-ions. These counter-ions can be reversibly exchanged with other ions of the same charge without altering the matrix. 1-Positively charged excha ...
Xian`s Southern Blot Protocol Using Digoxigenin Labeled Probe
... used Hybridization Buffer/Probe mix can be stored at -20°C and used repeatedly MEMBRANE DETECTION • wash 2X 15min at room temperature with 2X SSC, 0.1% SDS on rocker ...
... used Hybridization Buffer/Probe mix can be stored at -20°C and used repeatedly MEMBRANE DETECTION • wash 2X 15min at room temperature with 2X SSC, 0.1% SDS on rocker ...
Xian`s Southern Blot Protocol Using Digoxigenin Labeled Probe
... used Hybridization Buffer/Probe mix can be stored at -20°C and used repeatedly MEMBRANE DETECTION • wash 2X 15min at room temperature with 2X SSC, 0.1% SDS on rocker • wash 2X 15min at 42°C with 0.5X SSC, 0.1% SDS on rocker (washes can be modified to control stringency – this is fairly stringent) • ...
... used Hybridization Buffer/Probe mix can be stored at -20°C and used repeatedly MEMBRANE DETECTION • wash 2X 15min at room temperature with 2X SSC, 0.1% SDS on rocker • wash 2X 15min at 42°C with 0.5X SSC, 0.1% SDS on rocker (washes can be modified to control stringency – this is fairly stringent) • ...
HUMAN UTERUS TISSUE LYSATE For Research Use Only
... Buffer requirements for performing protein-protein interaction and ligand binding studies can vary significantly from RIPA buffer and may require modifications. In most cases, tissue lysates in RIPA buffer can be used, directly in standard ELISA and immunoprecipitation assays. This material has test ...
... Buffer requirements for performing protein-protein interaction and ligand binding studies can vary significantly from RIPA buffer and may require modifications. In most cases, tissue lysates in RIPA buffer can be used, directly in standard ELISA and immunoprecipitation assays. This material has test ...
DNA Electrophoresis Electrophoresis Electrophoresis using DNA
... Fast and sensitive silver staining [4] We use this staining protocol because it will fix also smaller DNA-fragments below 150 bp! This staining procedure gives also best results with denaturing, 7 M Urea containing gels! We recommend to use the Autostainer from GE, see fig.7. Our Stock Solutions: (T ...
... Fast and sensitive silver staining [4] We use this staining protocol because it will fix also smaller DNA-fragments below 150 bp! This staining procedure gives also best results with denaturing, 7 M Urea containing gels! We recommend to use the Autostainer from GE, see fig.7. Our Stock Solutions: (T ...
Buffers and its uses.
... changes in pH when small quantities of an acid or an alkali are added to it. An acidic buffer solution is simply one which has a pH less than 7. Acidic buffer solutions are commonly made from a weak acid and one of its salts - often a sodium salt. An alkaline buffer solution has a pH greater tha ...
... changes in pH when small quantities of an acid or an alkali are added to it. An acidic buffer solution is simply one which has a pH less than 7. Acidic buffer solutions are commonly made from a weak acid and one of its salts - often a sodium salt. An alkaline buffer solution has a pH greater tha ...
3P Color Buffer
... The 10X P-Green Buffer allows you to go directly from the thermal cycler to gel analysis. The buffer contains a compound that increases sample density, so that samples sink easily into the wells of an agarose gel. The 10X P-Green Buffer contains two dyes (yellow and blue) that separate to allow easy ...
... The 10X P-Green Buffer allows you to go directly from the thermal cycler to gel analysis. The buffer contains a compound that increases sample density, so that samples sink easily into the wells of an agarose gel. The 10X P-Green Buffer contains two dyes (yellow and blue) that separate to allow easy ...
Frequently Asked Questions: Agarose Gel Electrophoresis
... A6: Exposing a gel to staining agent after electrophoresis is complete results in even staining and provides clear visualization of DNA fragments.In addition, because staining solution can be used multiple times, only a small amount of dye is required to stain a large number of gels. In comparison, ...
... A6: Exposing a gel to staining agent after electrophoresis is complete results in even staining and provides clear visualization of DNA fragments.In addition, because staining solution can be used multiple times, only a small amount of dye is required to stain a large number of gels. In comparison, ...
Capillary electrophoresis
Capillary electrophoresis (CE) is a family of electrokinetic separation methods performed in submillimeter diameter capillaries and in micro- and nanofluidic channels. Very often, CE refers to capillary zone electrophoresis (CZE), but other electrophoretic techniques including capillary gel electrophoresis (CGE), capillary isoelectric focusing (CIEF), capillary isotachophoresis and micellar electrokinetic chromatography (MEKC) belong also to this class of methods. In CE methods, analytes migrate through electrolyte solutions under the influence of an electric field. Analytes can be separated according to ionic mobility and/or partitioning into an alternate phase via Non-covalent interactions. Additionally, analytes may be concentrated or ""focused"" by means of gradients in conductivity and pH.