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Mendelian Inheritance - DNALC::Protocols
Mendelian Inheritance - DNALC::Protocols

... Through cell division, this single fertilized egg will become a fetus, and eventually a newborn. Ask students from whom their parents inherited chromosomes. This should lead to the conclusion that DNA is passed down or inherited over generations, and that explains why certain physical traits are pre ...
FTv6_6_changes
FTv6_6_changes

... prepared by the Nomenclature Committee of the International Union of Biochemistry and Molecular Biology (NC-IUBMB) (published in Enzyme Nomenclature 1992, Academic Press, San Diego, or a more recent revision thereof). The format represents a string of four numbers separated by full stops; up to thre ...
(HPV) L1 gene DNA possibly bound to particulate aluminum
(HPV) L1 gene DNA possibly bound to particulate aluminum

Chapter 1: The Genetic Approach to Biology Questions for Chapter 1
Chapter 1: The Genetic Approach to Biology Questions for Chapter 1

... Wheat is a hexaploid result of three wild species coming together Three sets of chromosomes doubled in order to multiply Traits can be selected for such as gluten protein, stronger shells around seeds Bigger plants and bigger yield In peas, both cross=pollination and self-polination (selfing) are po ...
Cleavage of a model DNA replication fork by a Type I restriction
Cleavage of a model DNA replication fork by a Type I restriction

... idea that Mus81-Eme1/Mms4 endonuclease in eukaryotes can cleave a stalled replication fork (10,11), but contribution of the fork cleavage to fork restart is yet to be elucidated (11). Archaeal Hef that cleaves several branched forms of DNA in vitro was proposed to be involved in cutting off the arm c ...
Document
Document

... • 3’ poly A tail – plays a role in the export of the mRNA into the cytoplasm – after transcription – an enzyme adds 20 to 250 adenine nucleotides after the poly-adenylation signal sequence – also prevents degradation of the mRNA once its in the cytoplasm ...
Hypercholesterolemia
Hypercholesterolemia

... the LDL receptor gene is then amplified by the polymerase chain reaction (PCR). The amplified DNA is analyzed to see if there is a mutation. To analyze the DNA, investigators use restriction enzymes to cut the DNA in specific places. By examining the sizes of the DNA fragments obtained after exposing t ...
Molecular Biology and Genetics
Molecular Biology and Genetics

... DNA must replicate (copy) itself so that each resulting cell after mitosis and cell division has the same DNA as the parent cell. DNA replication occurs during the S phase of the cell cycle, before mitosis and cell division. The base pairing rules are crucial for the process of replication. DNA repl ...
QUESTIONS AND ANSWER TO PROBLEM SETS
QUESTIONS AND ANSWER TO PROBLEM SETS

... Answer: There are many ethical issues associated with human cloning. Is it the wrong thing to do? Does it conflict an individual’s religious views? And so on. FIGURE 1.3 Concept check: Why is it useful to sort male and female mosquitos? Answer: By sorting the mosquitos, sterile males can be released ...
Looking at long molecules in solution: what happens when they are
Looking at long molecules in solution: what happens when they are

... Received 3rd April 2006, Accepted 24th May 2006 First published as an Advance Article on the web 14th June 2006 DOI: 10.1039/b604810m Knowing the structure of a molecule is one of the keys to deducing its function in a biological system. However, many biomacromolecules are not amenable to structural ...
Genes can be switched on and off by the protein CTCF
Genes can be switched on and off by the protein CTCF

Slide 1
Slide 1

...  When a plant or animal is bred with a plant or animal from different stock, the process is known as hybridization. There are numerous reasons to create hybrids, including increasing genetic diversity and breeding for specific traits. It is frequently practiced in agriculture, to make stronger, hea ...
Candidatus Liberibacter asiaticus - Southwest Florida Research and
Candidatus Liberibacter asiaticus - Southwest Florida Research and

Reviews - Mi Portal
Reviews - Mi Portal

... After a double-strand break (DSB) is created, the ends are resected and one of the 39 single-stranded ends can invade an intact template. Strand invasion requires the participation of the Rad51p strand exchange protein and a number of associated proteins, including Rad52p, Rad54p and Tid1p, discusse ...
CHAPTER 7 DNA Mutation, DNA Repair and Transposable Elements
CHAPTER 7 DNA Mutation, DNA Repair and Transposable Elements

... 1. DNA replication errors can be either point mutations, or small insertions or deletions. 2. Base-pair substitution mutations can result from “wobble” pairing. Bases are normally in the keto form, but sometimes can undergo tautomeric shift to form the abnormal (enol) form. The enol form can hydroge ...
Clone
Clone

CHAPTER 9
CHAPTER 9

... and strC genes confer the ability to synthesize proline and confer streptomycin resistance, respectively.) A recipient strain is proA– and strC – and resistant to tetracycline. After transformation, the bacteria were first streaked on plates containing proline, streptomycin, and tetracycline. Coloni ...
Document
Document

... • 3’ poly A tail – plays a role in the export of the mRNA into the cytoplasm – after transcription – an enzyme adds 20 to 250 adenine nucleotides after the poly-adenylation signal sequence – also prevents degradation of the mRNA once its in the cytoplasm ...
Title A Fluorescently Labeled, Hyperbranched Polymer
Title A Fluorescently Labeled, Hyperbranched Polymer

Name that Gene Project The National Center for Biotechnology
Name that Gene Project The National Center for Biotechnology

... A) For each of the top three matches, click on the Accession link to the right. This will take you to a page with more information about the sequence. Report in the table on the data sheet the entry that appears next to the heading SOURCE ORGANISM. B) In the world of molecular biology, what is a vec ...
"False But Highly Persuasive": How Wrong Were the Probability
"False But Highly Persuasive": How Wrong Were the Probability

Codon Dictionary Worksheet
Codon Dictionary Worksheet

... So, if the mRNA codon is UUU, what tRNA anticodon is complementary? (Answer: AAA) If the mRNA codon is UAC, what tRNA anticodon is complementary? (Answer #4 below) If the mRNA codon is GGU, what tRNA anticodon is complementary? (Answer #5 below) If the tRNA anticodon is UAC, with which mRNA codon do ...
Implications of DNA replication for eukaryotic gene expression
Implications of DNA replication for eukaryotic gene expression

... The period in the eukaryotic cell cycle when the genome is duplicated (S phase) is crucially important for both establishing and maintaining programs of gene activity. The majority of genes in a proliferating cell of a defined type or line continually retain the same states of transcriptional activi ...
The presence of two UvrB subunits in the UvrAB complex ensures
The presence of two UvrB subunits in the UvrAB complex ensures

Understanding the role of markers in locating genes: Flowering Time
Understanding the role of markers in locating genes: Flowering Time

... Statistical analysis using student t-test The task of the students is to perform a two-tailed student t-test on each of the markers in a given data set to determine which biomarker is significantly linked with the gene or QTL associated with flowering time. It will provide the students the opportuni ...
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DNA supercoil



DNA supercoiling refers to the over- or under-winding of a DNA strand, and is an expression of the strain on that strand. Supercoiling is important in a number of biological processes, such as compacting DNA. Additionally, certain enzymes such as topoisomerases are able to change DNA topology to facilitate functions such as DNA replication or transcription. Mathematical expressions are used to describe supercoiling by comparing different coiled states to relaxed B-form DNA.As a general rule, the DNA of most organisms is negatively supercoiled.
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