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honors biology: final exam review
honors biology: final exam review

... Know the basic structure of DNA and RNA, especially the differences between them o Number of strands, sugar, bases o Base-pairing rules Know the goal and basic process of DNA replication o Template, semiconservative model o Major enzymes involved and their functions  DNA helicase ...
2) Chromatin = uncoiled DNA
2) Chromatin = uncoiled DNA

... attaches at a specific location on DNA 2. The enzyme then causes the DNA strands to separate from one another and allow one of the DNA strands to be ________________ 3. mRNA nucleotides are floating around in the nucleus find their complement on the DNA stand and _______________together. This is pos ...
How to accelerate protein search on DNA: Location and dissociation
How to accelerate protein search on DNA: Location and dissociation

... (on the DNA) modes. But the most paradoxical observation is that protein molecules spend most of the search time (≥90 − 99%) on the DNA chain where they diffuse very slowly.7, 8, 12 It is not clear then how the fast search can be achieved in this case. Several theoretical ideas that point out to the ...
Mutations
Mutations

... Chromosomal Mutations • Nondisjunction = failure of homologous chromosomes to separate during meiosis resulting in gametes (egg or sperm) with too few or too many chromosomes • Animation: ...
And can we predict these positions by analysing
And can we predict these positions by analysing

... • Nucleosome organization is encoded ~50% by genome sequence. And this is conserved across species. • Genomes encode the positioning and stability of nucleosomes in regions that are critical for gene regulation and for other specific chromosome functions. • Confirms work of Satchwell et al. where si ...
15.2 Recombinant DNA
15.2 Recombinant DNA

Anticancer Antibiotics
Anticancer Antibiotics

... between the two cis-oxygen functionalities at these positions stabilizes the preferred half-chair conformation of the D-ring. The amino sugar residue of the various anthracyclines is an essential requirement for bioactivity. Among the various SAR studies involving the carbohydrate core, it has been ...
DNA
DNA

... - In eukaryotes, DNA does not exist in isolation but rather it is wound/wrapped (or negatively supercoiled) around bead-like protein complexes called “histones” to form what has come to be known as a “nucleosome” - Nucleosomes represent the first building blocks for packaging/folding DNA into a high ...
File - The Tarrytown Meetings
File - The Tarrytown Meetings

DNA - Trinity Regional School
DNA - Trinity Regional School

Geometrical ordering of DNA in bacteria
Geometrical ordering of DNA in bacteria

... Figure 1. Average subcellular position of genes as function of their position on the chromosome in C. crescentus and E. coli as obtained from numerical simulations of compacted DNA. (A and B) show the position of genes along the cell axis as function of their position on the chromosomal map for an a ...
university of oslo
university of oslo

... a) Stringent response - a response in bacteria to lack of nutrients. If bacteria cannot grow due to low levels of nutrients, transcription of genes (particularly tRNA and ribosomal RNA genes) is reduced to a few percent of the normal level. The response is mediated by the DksA protein and alarmones ...
10 gene expression: transcription
10 gene expression: transcription

... The presence of two Laf proteins can be explained by alternative splicing of the laf+ mRNA. So let’s first try to figure out the coding region of the laf+ gene. The initiation codon will have to be an AUG. There are two codons early on: at positions 83–85 and 118–120. The latter is almost immediatel ...
A system in mouse liver for the repair of O6
A system in mouse liver for the repair of O6

... any recovery of activity. We next contrasted the specificity of release of the methylated bases. These data are illustrated in Figure 2. It is apparent that an almost complete disappearance of 0 -methylguanine was observed in the incubation as compared to the control. In this case, 93% of the origin ...
Chapter 18
Chapter 18

... Sticky ends can bind by base pairing to other sticky ends. Fragments from different sources can be joined. Then ligase catalyzes formation of covalent bonds between adjacent nucleotides at fragment ends, joining them to form a single, larger molecule. ...
6. DNA transcription/translation
6. DNA transcription/translation

... DNA Replication and Protein Synthesis A. DNA as the Genetic Material 1. The search for genetic material led to DNA. 2. Watson and Crick discovered the double helix by building models to conform to X-ray data. ...
Rapid Communication: Mapping of the Titin (TTN) Gene to Pig
Rapid Communication: Mapping of the Titin (TTN) Gene to Pig

KEY TERMS FOR Characteristics of Life
KEY TERMS FOR Characteristics of Life

... 4. Compare and contrast DNA and RNA in terms of long name, type of sugar, number of strands, what part of the cell it is found in (nucleus and / or cytoplasm) and names of bases 5. Explain what is actually happening when the DNA unzips. 6. Explain the basic process of translation (where in the cell ...
PI-40069
PI-40069

... Biotium as an improved version of our popular PMA dye. The dye is weakly fluorescent by itself but becomes highly fluorescent upon binding to nucleic acids. It preferentially binds to dsDNA with high affinity. Upon photolysis, the photoreactive azido group on the dye is converted to a highly reactiv ...
Chapter 4B
Chapter 4B

... sequence of the protein. Each amino acid is selected based on the order of triplet codons in mRNA. Transfer RNA (tRNA) converts the information in mRNA codons into the amino acid sequence of the protein. tRNAs carry amino acids specified by the codons and base pair with the codons via their anticodo ...
Genetic mapping RFLP: Restriction Fragment Length
Genetic mapping RFLP: Restriction Fragment Length

... – Find the difference between the RFLP markers of family members with the disease and family members not having the disease. – It is likely that the RFLP marker that consistently differ is on the gene responsible for the disease, since family members have more or less the same genetic characteristic ...
1 A. You have the following piece of genomic DNA with the two
1 A. You have the following piece of genomic DNA with the two

THINK ABOUT IT
THINK ABOUT IT

... The Molecular Basis of Heredity One of the most interesting discoveries of molecular biology is the nearuniversal nature of the genetic code. Although some organisms show slight variations in the amino acids assigned to particular codons, the code is always read three bases at a time and in the same ...
Taq DNA Polymerase
Taq DNA Polymerase

Pogil activity DNA to protein
Pogil activity DNA to protein

... be in the nucleus of a cell along with many thousands of other genes arranged on chromosomes. This gene is much smaller than genes are in real life, but it will give you the idea of how this process works. During a process called transcription, DNA in the nucleus is used to make a molecule called mR ...
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DNA supercoil



DNA supercoiling refers to the over- or under-winding of a DNA strand, and is an expression of the strain on that strand. Supercoiling is important in a number of biological processes, such as compacting DNA. Additionally, certain enzymes such as topoisomerases are able to change DNA topology to facilitate functions such as DNA replication or transcription. Mathematical expressions are used to describe supercoiling by comparing different coiled states to relaxed B-form DNA.As a general rule, the DNA of most organisms is negatively supercoiled.
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