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Methylation Dynamics in the Early Mammalian Embryo - Beck-Shop
Methylation Dynamics in the Early Mammalian Embryo - Beck-Shop

14 Family planning
14 Family planning

... Unintended pregnancies are those in which conception was not intended by the female sexual partner. Unintended pregnancies are the primary cause of induced abortion, resulting in about 42 million induced abortions per year. Unintended pregnancy is also linked to higher rates of maternal and infant ...
Polypeptide Synthesis -Making Proteins
Polypeptide Synthesis -Making Proteins

Germline MUTYH (MYH) mutations in Portuguese individuals with
Germline MUTYH (MYH) mutations in Portuguese individuals with

... adenomatous polyposis, who tested negative for APC germinal mutations (Table 1). In other studies, performed in the same class of patients, the frequency of MYH biallelic mutation varied between 7 and 33.8 % (Sieber et al., 2003; Sampson et al., 2003; Jones et al., 2002). Although ethnic or geograph ...
Extrachromosomal DNA Transformationof Caenorhabditis elegans
Extrachromosomal DNA Transformationof Caenorhabditis elegans

... foreign DNA sequences in the transformed worm cells were measured by quantitative hybridization analysis. DNA preparations from transformed populations were spotted onto triplicate nitrocellulose filters. The filters were hybridized with 32P-labeled pBR322 DNA, bacteriophage DNA containing a single- ...
Get cached PDF
Get cached PDF

... a male Border Collie and a female Newfoundland. The Newfoundland parent had a small patch of white on the chest and was otherwise completely black (Figure 1a). The Border Collie used in this cross had markings characteristic for the breed - black with white markings on the face, chest, neck, tail ti ...
Raised Haemoglobin F (HbF) Level in Haemoglobinopathies: an
Raised Haemoglobin F (HbF) Level in Haemoglobinopathies: an

... problems In patients with sickle cell disease and beta thalassemia, the presence of this polymorphism is associated with higher Hb F levels. With this polymorphic site, there is an increase in the proportion of γG chains resulting in a γG:γA ratio similar to that seen at birth (70:30)[63]-[64]. The ...
Rhesus Negative - Gateshead Health NHS Foundation Trust
Rhesus Negative - Gateshead Health NHS Foundation Trust

... Rhesus status is important during pregnancy. If blood cells from a Rhesus Positive baby get into the bloodstream of a Rhesus Negative mother, she will react by producing Anti-D antibodies as though the baby’s blood was a foreign body. The most common time for this to happen is during birth, but it c ...
Biology Ch. 13
Biology Ch. 13

... A person wishes to raise guinea pigs with black fur, the dominant trait. She selects a male black guinea pig and performs a test cross with a female that has white fur, the recessive trait. What is the black guinea pig’s genotype if any of the offspring are white? ...
THE CHASM BETWEEN THE HUMAN AND CHIMPANZEE GENOMES
THE CHASM BETWEEN THE HUMAN AND CHIMPANZEE GENOMES

... not in chimp, and a number of these characterized duplications contained genes. Of 177 gene sequences in these repeats, 88 were duplicated in human and not chimpanzee while 94 were duplicated in chimpanzee and not human. Since gene copy number is a major regulator of gene expression, this was a sign ...
Classical (Mendelian) Genetics
Classical (Mendelian) Genetics

... • A person can receive blood only when the donor's blood type does not contain any surface antigen the recipient does not. This is because the recipient has antibodies which will attack any foreign surface protein. • Thus, Type AB can accept any blood types because it will not attack A or B surface ...
Human-Genetics-Concepts-and-Applications-9E
Human-Genetics-Concepts-and-Applications-9E

... direct-to-consumer genetic test panel and discovers that he has inherited gene variants that are associated with increased risk of alcoholism. He reports these facts during an interview for purchasing a life insurance policy, because he thinks that the Genetic Information Nondiscrimination Act will ...
Structural basis for the inhibition of human alkyladenine Please share
Structural basis for the inhibition of human alkyladenine Please share

GENECLEAN® Kit
GENECLEAN® Kit

... When working with radio-labeled DNA, add 1 µl of Label Block to each 10 µl of GLASSMILK® and incubate for 5 minutes at room temperature before adding to the DNA/NaI solution in the next step. 5. Add GLASSMILK® to the DNA/NaI solution as calculated in Step 3. Vortex gently or stir with a pipet tip to ...
BIOL 112 – Principles of Zoology
BIOL 112 – Principles of Zoology

... of the chromosomes within the human genome. Each probe is labeled with a fluorescent color that is designated for a specific chromosome.. the probes hybridize, the fluorescent probes essentially paint the full set of chromosomes, can be analyzed to determine whether any of them exhibits translocatio ...
Chpt2_Struc_Nucleic_Acids.doc
Chpt2_Struc_Nucleic_Acids.doc

... the genetic material, and these encode the macromolecules that function in the cell. DNA is metabolically and chemically more stable than RNA. One tends to find RNA genomes in organisms that have a short life span. Even prions are not exceptions to this rule that genomes are composed of nucleic acid ...
UK NEQAS FOR MOLECULAR GENETICS UK NATIONAL …
UK NEQAS FOR MOLECULAR GENETICS UK NATIONAL …

... Mitochondrial diseases Myotonic dystrophy type 1 Spinal muscular atrophy CF testing on blood spots Molecular Rapid Aneuploidy EQA UK NEQAS ...
8067 Perform a polymerase chain reaction (PCR)
8067 Perform a polymerase chain reaction (PCR)

... Providers and Industry Training Organisations, which have been granted consent and which are assessing against unit standards must engage with the moderation system that applies to those standards. Requirements for consent to assess and an outline of the moderation system that applies to this standa ...
Purpose of Y-chromosome Analysis - College of Letters, Arts, and
Purpose of Y-chromosome Analysis - College of Letters, Arts, and

... A Y-chromosome DNA profile can provide unique information about the paternal lineage of a particular individual. This profile is generated when specific, individually variable regions of Y-chromosome DNA are tested using established methods. If no genetic changes (mutations) have occurred between ge ...
zChap04_140901 - Online Open Genetics
zChap04_140901 - Online Open Genetics

... strategy of mutant screening has been used very effectively to identify and understand the molecular components of hundreds of different biological processes. For example, to find the basic biological processes of memory and learning, researchers have screened mutagenized populations of Drosophila t ...
Omega-3s in Pregnancy - University of Wyoming
Omega-3s in Pregnancy - University of Wyoming

... visual development and may have other benefits, guidelines recommend at least 200 mg of DHA per day for pregnant women.1 Omega-3s and Allergic Disease in Infants Possible Mechanism of Action: DHA and EPA show immunomodulatory properties. The omega-3 fatty acid may “mitigate the severity of the aller ...
ACEMBL System:
ACEMBL System:

... promoters T7 and Lac, as well as the T7 terminator element (Illustr.1, 10). The T7 system is currently most commonly used; it requires bacterial strains which contain a T7 polymerase gene in the E. coli genome. The Lac promoter is a strong endogenous promoter which can be utilized in most strains. A ...
DNA Duplication Associated with Charcot-Marie-Tooth Disease Type 1A. Lupski, et al., 1991 Cell, Vol. 66, 219-232, July 26, 1991,
DNA Duplication Associated with Charcot-Marie-Tooth Disease Type 1A. Lupski, et al., 1991 Cell, Vol. 66, 219-232, July 26, 1991,

... Associated with CMTIA We screened CMTl A-linked 17p DNA probes for the presence of simple sequence repeats such as (GT),, which are known to be highly polymorphic and can be rapidly analyzed by the polymerase chain reaction (PCR) (Weber and May, 1989; Litt and Luty, 1989). (CT), sequences were ident ...
BIOTECH FALL FINAL review16
BIOTECH FALL FINAL review16

... 21. Explain what SDS is and how it used in our DNA and protein extraction labs. 22. Draw a simple DNA gel and explain what is occurring using the word electrophoresis. Identify who/what is involved in terms of DNA. 23. Draw an adenine nucleotide and label its parts. 24. What is the central dogma of ...
Radiographs: Angulate
Radiographs: Angulate

... Congenital: present at birth -- Doesn’t have to be inherited, e.g. congenital syphilis Familial: runs in families (genetics may be unknown) Hereditary: derived from gametes of one’s own parents Polygenic (multifactorial) inheritance: multiple genes involved, multiple patterns of inheritance Polymorp ...
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Cell-free fetal DNA

Cell-free fetal DNA (cffDNA) is fetal DNA circulating freely in the maternal blood stream. It can be sampled by venipuncture on the mother. Analysis of cffDNA provides a method of non-invasive prenatal diagnosis.cffDNA originates from the trophoblasts making up the placenta. It is estimated that 2-6% of the DNA in the maternal blood is fetal in origin. The fetal DNA is fragmented and makes its way into the maternal bloodstream via shedding of the placental microparticles into the maternal bloodstream (figure 1). Studies have shown that cffDNA can first be observed as early as 7 weeks gestation, and the amount of cffDNA increases as the pregnancy progresses. cffDNA diminishes quickly after the birth of the baby, so that it is no longer detectable in the maternal blood approximately 2 hours after birth. cffDNA is significantly smaller than the maternal DNA in the bloodstream, with fragments approximately 200bp in size. Many protocols to extract the fetal DNA from the maternal plasma use its size to distinguish it from the maternal DNA.Studies have looked at, and some even optimized, protocols for testing non-compatible RhD factors, sex determination for X-linked genetic disorders and testing for single gene disorders. Current studies are now looking at determining aneuploidies in the developing fetus. These protocols can be done earlier than the current prenatal testing methods, and have no risk of spontaneous abortion, unlike current prenatal testing methods. Non-invasive prenatal diagnosis (NIPD) has been implemented in the UK and parts of the US; it has clear benefits above the standard tests of chorionic villi sample (CVS) and amniocentesis which have procedure-related miscarriage risks of about 1 in 100 pregnancies and 1 in 200 pregnancies, respectively.As a method of prenatal diagnosis, cell-free fetal DNA techniques share the same ethical and practical issues, such as the possibility of prenatal sex discernment and sex selection.
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