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Understanding Mutation (PowerPoint) WVU 2013
Understanding Mutation (PowerPoint) WVU 2013

...  Students will formulate a working definition of a mutation and use it assess whether a mutation has occurred. Tidbit One- 30 second paper, Guided-Inquiry Clicker Qs  Given various scenarios, students will be able to evaluate whether a mutation will lead to a phenotypic variation. Tidbit Two- Doub ...
Se talking2
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... Bulked segregant analysis is a rapid procedure for identifying interesting genes in specific regions of the genome. The method involves comparing two pooled DNA samples of individuals from a segregating population originating from a single cross. Within each pool, or bulk, the individuals are identi ...
Biology EOC preparation
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... 3.03: Interpret and predict patterns of inheritance 1. Explain phenotypes and genotypes. 2. Explain how Mendel impacted our knowledge of genetics. 3. What is a karyotype and how can we use it? 4. Explain the relationship between dominant and recessive traits. 5. Explain incomplete dominance. 6. Expl ...
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... b. DNA c. Proteins d. Lipids e. Salt 3. Which is the correct term for compounds that do mix with water? a. phospholipids b. hydrophobic c. hydrophilic d. protein e. hydrogen bonded 4. Which of the following do nucleic acids and proteins have in common? a. They are both made of amino acids. b. Their ...
Chromosome Microarray (CMA) Pre-Test Patient
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... were not found. A new enhanced test for looking at chromosomes is available and is called  chromosome microarray analysis.    What is Chromosome Microarray Analysis (CMA)?   CMA is an advance in technology that allows the detection of chromosome imbalances that are  smaller than can be detected by k ...
CXA 300 Human Molecular Biology Laboratory Manual Semester 1
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... PCR proceeds, the exonucleoase activity of DNA polymerase cleaves the quencher from the fluorophore, allowing fluorescence signal to be detected. Because binding of each probe occurs in a sequence-specific manner, and each allele-specific probe is bonded to a different fluorophore, the presence of e ...
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Enzyme Mechanisms - Illinois Institute of Technology
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... Gel soaked in base to denature duplexes pH readjusted to neutral Sheet of absorbent material placed atop the gel Salt solution is drawn across the gel, perp to the electrophoretic direction, in various ways to carry the DNA onto the sheet Sheet is dried in an oven to tightly attach the DNA to it Inc ...
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... revert to being F+ when the F plasmid DNA incorporated into the Hfr chromosome has a crossover and loops out of the chromosome forming an F plasmid once again. Sometimes the looping-out and crossing-over process doesn’t happen at the proper place. When this happens, a piece of the bacterial chromoso ...
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Experiment 8 - WordPress.com
Experiment 8 - WordPress.com

... 7)If you spread 100 μl of the +pGLO transformation solution onto an LB plate, what  should you see after incubating the plate at 37­degrees for a few days and why? If you used  the velvet stamping method demonstrated in lab 3 to replica­plate the growth from this LB  plate onto an LB/Amp plate, what ...
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... 12. In horses, the allele for straight hair (B) is dominant to the allele for curly hair (b). Which of these sets of parents can produce offspring with curly hair? A. A heterozygous male with straight hair and a homozygous female with straight hair B. A homozygous male with curly hair and a homozygo ...
Chapters 16-17 (DNA and protein synthesis)
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... Alternative RNA splicing allows some genes to produce different polypeptides. Exons may code for polypeptide domains, which are the functional parts of a protein (such as binding and active sites). ...
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... These elements transpose via DNA intermediates such as: Ac/Ds and Spm in plants, P elements in animals, Tn in bacteria A common feature of DNA elements is the flanking of the element by short inverted repeat sequences The enzyme transposase recognizes these sequences, creates a stem/loop structure e ...
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Monster Central Dogma - Lincoln Park High School
Monster Central Dogma - Lincoln Park High School

... 5. Suggest a substitution mutation in the DNA that would cause the first amino acid in the “# of Eyes” gene to change from alanine (Ala) to valine (Val). Write the original DNA codon, then the mutated DNA codon. (1) 6. There is a substitution mutation in the gene for Fangs in which the first DNA bas ...
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PCR Techniques

... Fusion PCR • What if you want to join two genes together (or a promoter with a gene) • Can use megaprimer mutagenesis and clone genes in one at a time • 1. Design internal primers that have 5’ overhangs that are complementary to fusion gene (Tm>55) • 2. Do PCR of each gene individually • 3. Do gel ...
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Cell-free fetal DNA

Cell-free fetal DNA (cffDNA) is fetal DNA circulating freely in the maternal blood stream. It can be sampled by venipuncture on the mother. Analysis of cffDNA provides a method of non-invasive prenatal diagnosis.cffDNA originates from the trophoblasts making up the placenta. It is estimated that 2-6% of the DNA in the maternal blood is fetal in origin. The fetal DNA is fragmented and makes its way into the maternal bloodstream via shedding of the placental microparticles into the maternal bloodstream (figure 1). Studies have shown that cffDNA can first be observed as early as 7 weeks gestation, and the amount of cffDNA increases as the pregnancy progresses. cffDNA diminishes quickly after the birth of the baby, so that it is no longer detectable in the maternal blood approximately 2 hours after birth. cffDNA is significantly smaller than the maternal DNA in the bloodstream, with fragments approximately 200bp in size. Many protocols to extract the fetal DNA from the maternal plasma use its size to distinguish it from the maternal DNA.Studies have looked at, and some even optimized, protocols for testing non-compatible RhD factors, sex determination for X-linked genetic disorders and testing for single gene disorders. Current studies are now looking at determining aneuploidies in the developing fetus. These protocols can be done earlier than the current prenatal testing methods, and have no risk of spontaneous abortion, unlike current prenatal testing methods. Non-invasive prenatal diagnosis (NIPD) has been implemented in the UK and parts of the US; it has clear benefits above the standard tests of chorionic villi sample (CVS) and amniocentesis which have procedure-related miscarriage risks of about 1 in 100 pregnancies and 1 in 200 pregnancies, respectively.As a method of prenatal diagnosis, cell-free fetal DNA techniques share the same ethical and practical issues, such as the possibility of prenatal sex discernment and sex selection.
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