Science at the heart of medicine William R. Jacobs, Jr., Ph.D.
... a mycobacterial phage to make a genetic tool he named "the shuttle phasmid." Because this hybrid DNA can replicate itself as a plasmid in E. coli and as a phage in Mycobacterium, it can shuttle genes from one to the other, including genes that have been inserted into E. coli in the lab. Investigator ...
... a mycobacterial phage to make a genetic tool he named "the shuttle phasmid." Because this hybrid DNA can replicate itself as a plasmid in E. coli and as a phage in Mycobacterium, it can shuttle genes from one to the other, including genes that have been inserted into E. coli in the lab. Investigator ...
1 - western undergrad. by the students, for the students.
... Alberta. She estimates that the diploid genome size of the mammoth to be approximately 1 billion base pairs. Through a novel experimental technique she is able to recover a stretch of DNA 5,432 nucleotides long. How much information (in bits) was she was able to recover from the long extinct mammoth ...
... Alberta. She estimates that the diploid genome size of the mammoth to be approximately 1 billion base pairs. Through a novel experimental technique she is able to recover a stretch of DNA 5,432 nucleotides long. How much information (in bits) was she was able to recover from the long extinct mammoth ...
EAWAG news 56e: Genomic Islands and Horizontal Gene Transfer
... other proteins showed that it was related to integrases from bacterial viruses (bacteriophages), which place their genomes into the chromosomes of the infected cells, and to integrases from so-called genomic islands (see glossary) [3]. The gene for the B13 integrase is situated at the right end of t ...
... other proteins showed that it was related to integrases from bacterial viruses (bacteriophages), which place their genomes into the chromosomes of the infected cells, and to integrases from so-called genomic islands (see glossary) [3]. The gene for the B13 integrase is situated at the right end of t ...
Informed consent.
... chromosome containing between 700 and 3,000 genes. Each gene consists of fragments of DNA sequence called exons, which contain the information necessary for the synthesis of proteins, and introns, interspersed with exons, that carry out a different function. They can be represented like the followin ...
... chromosome containing between 700 and 3,000 genes. Each gene consists of fragments of DNA sequence called exons, which contain the information necessary for the synthesis of proteins, and introns, interspersed with exons, that carry out a different function. They can be represented like the followin ...
Nucleic Acid Interaction
... It is apparent from crystal structures of these protein-DNA complexes that the differential affinities of 434 repressor and cro for the different operator regions are not determined by sequence-specific interactions between amino acid side chains of the recognition helix and base pairs in the major ...
... It is apparent from crystal structures of these protein-DNA complexes that the differential affinities of 434 repressor and cro for the different operator regions are not determined by sequence-specific interactions between amino acid side chains of the recognition helix and base pairs in the major ...
The Human Genome Project – Part 2
... • Celera used publicly funded sequence to produce its published draft of the human genome • Scientists who worked on the map-based effort claimed Celera couldn’t have produced a draft without access to the public sequence • Celera scientists claim that they could have produced an accurate draft even ...
... • Celera used publicly funded sequence to produce its published draft of the human genome • Scientists who worked on the map-based effort claimed Celera couldn’t have produced a draft without access to the public sequence • Celera scientists claim that they could have produced an accurate draft even ...
DNA - Wise Science
... • A whole chromosome is too large for scientists to study a particular gene easily, so they had to find a way to get much smaller pieces of DNA. • In Gel Electrophoresis, an electrical current is used to separate a mixture of DNA fragments from each other. • A sample of DNA is loaded into a gel, whi ...
... • A whole chromosome is too large for scientists to study a particular gene easily, so they had to find a way to get much smaller pieces of DNA. • In Gel Electrophoresis, an electrical current is used to separate a mixture of DNA fragments from each other. • A sample of DNA is loaded into a gel, whi ...
Methods, Applications and Policy for Agriculture OVERVIEW
... • The latest additions to the tool kit are the CRISPR/Cas RNA-guided nucleases. In this case, recognition is mediated by a guide RNA. − In addition, only a single protein, Cas9, is required for DNA cleavage. It doesn’t need to be modified when the target is changed; only the guide RNA needs to be ch ...
... • The latest additions to the tool kit are the CRISPR/Cas RNA-guided nucleases. In this case, recognition is mediated by a guide RNA. − In addition, only a single protein, Cas9, is required for DNA cleavage. It doesn’t need to be modified when the target is changed; only the guide RNA needs to be ch ...
GENETICS
... One base is replaced by another base; this is also called a point mutation ACGUCAGUA Threonine—Serine—Valine ACGUUAGUA Threonine—Leucine—Valine Depending on where the mutation occurs, it may have no affect on the protein ACGUCAGUA Threonine—Serine—Valine ACGUCGGUA Threonine—Serine—Valine Wob ...
... One base is replaced by another base; this is also called a point mutation ACGUCAGUA Threonine—Serine—Valine ACGUUAGUA Threonine—Leucine—Valine Depending on where the mutation occurs, it may have no affect on the protein ACGUCAGUA Threonine—Serine—Valine ACGUCGGUA Threonine—Serine—Valine Wob ...
computational biology
... with no function, or perhaps the mutation occurs in a protein-coding region, but ends up not affecting the amino acid sequence of the protein. • Small change occurs in phenotype. A single mutation caused this cat's ears to curl backwards slightly. • Big change occurs in phenotype. A single mutation ...
... with no function, or perhaps the mutation occurs in a protein-coding region, but ends up not affecting the amino acid sequence of the protein. • Small change occurs in phenotype. A single mutation caused this cat's ears to curl backwards slightly. • Big change occurs in phenotype. A single mutation ...
Blueprint of Life
... o In bats, the limb is modified to form a wing with the fingers extended and skin stretched between each finger. Whales have within their single paddle-like fin a fully formed pentadactyl limb. o Inherited this from aquatic ancestors, the lobe-finned fish E.g. xylem o Ferns, conifers and flowering p ...
... o In bats, the limb is modified to form a wing with the fingers extended and skin stretched between each finger. Whales have within their single paddle-like fin a fully formed pentadactyl limb. o Inherited this from aquatic ancestors, the lobe-finned fish E.g. xylem o Ferns, conifers and flowering p ...
Answer Key - Iowa State University
... Acids and 64 possible codons (4^3). This means that many Amino Acids are represented by multiple codon triplets. However separate species are able to utilize certain codons better due to their specific cell machinery. If we know how often a given triplet is used in known genes for that organism, we ...
... Acids and 64 possible codons (4^3). This means that many Amino Acids are represented by multiple codon triplets. However separate species are able to utilize certain codons better due to their specific cell machinery. If we know how often a given triplet is used in known genes for that organism, we ...
DBMS-based EST Clustering and Profiling for Gene Expression
... local DBMS-based management of all kinds of data, in particular sequence and annotation, sequence similarity and cluster pre-computation of sequence similarities, clusters: performance for online analysis improvement and automation of the previous in silico differential display ...
... local DBMS-based management of all kinds of data, in particular sequence and annotation, sequence similarity and cluster pre-computation of sequence similarities, clusters: performance for online analysis improvement and automation of the previous in silico differential display ...
Mapping Regulatory Network from a Model Organism to a Non
... Determination of regulatory networks from available data is one of the major challenges in bioinformatics research. A regulatory network of an organism is represented by a set of genes and their regulatory relationships, which indicate how a gene or a group of genes affect (inhibit or activate) prod ...
... Determination of regulatory networks from available data is one of the major challenges in bioinformatics research. A regulatory network of an organism is represented by a set of genes and their regulatory relationships, which indicate how a gene or a group of genes affect (inhibit or activate) prod ...
Course Competency Learning Outcomes
... Discussing the progression of discovery from Classical to Modern Genetics. Defining basic concepts of Classical Genetics. Describing Mendel’s experimental design. Utilizing conventional Mendelian genetic terminology. Explaining Mendel’s principles of segregation, and independent assortment. Solving ...
... Discussing the progression of discovery from Classical to Modern Genetics. Defining basic concepts of Classical Genetics. Describing Mendel’s experimental design. Utilizing conventional Mendelian genetic terminology. Explaining Mendel’s principles of segregation, and independent assortment. Solving ...
Horizontal gene transfer and bacterial diversity
... How can one establish whether a new trait or a specific region of a genome is the result of a horizontal transfer? Obviously, it may not always be possible to have direct experimental evidence for conversion of a deficient strain in the presence of an appropriate donor. But genes acquired through HG ...
... How can one establish whether a new trait or a specific region of a genome is the result of a horizontal transfer? Obviously, it may not always be possible to have direct experimental evidence for conversion of a deficient strain in the presence of an appropriate donor. But genes acquired through HG ...
Lesson Objectives: You must be comfortable doing these items:
... RNA helps build proteins. Proteins, in turn, determine the structure and function of all your cells. Proteins consist of chains of amino acids. A protein’s structure and function depends on the sequence of its amino acids. Instructions for this sequence are encoded in DNA. In eukaryotic cells, chrom ...
... RNA helps build proteins. Proteins, in turn, determine the structure and function of all your cells. Proteins consist of chains of amino acids. A protein’s structure and function depends on the sequence of its amino acids. Instructions for this sequence are encoded in DNA. In eukaryotic cells, chrom ...
DNA Polymerase
... code for proteins along with introns that do not Because the initial mRNA, called a pre-RNA, includes the noncoding introns, it must be processed before it can be read by the tRNA While the mRNA is still in the nucleus, the introns are removed from the pre-RNA The exons that remain are joined ...
... code for proteins along with introns that do not Because the initial mRNA, called a pre-RNA, includes the noncoding introns, it must be processed before it can be read by the tRNA While the mRNA is still in the nucleus, the introns are removed from the pre-RNA The exons that remain are joined ...
Nucleic Acids and Chromatin
... 1. DNA and RNA are polymers of nucleotides (polynucleotides). Nucleotides contain a base, a sugar and a phosphate. a. The base is either a purine (A & G), or a pyrimidine (T & C for DNA or U & C for RNA). In many cases the bases contain chemical modifications which may affect their function. Some of ...
... 1. DNA and RNA are polymers of nucleotides (polynucleotides). Nucleotides contain a base, a sugar and a phosphate. a. The base is either a purine (A & G), or a pyrimidine (T & C for DNA or U & C for RNA). In many cases the bases contain chemical modifications which may affect their function. Some of ...
Regulating Gene Expression
... At their simplest, these are a piece of genetic material with a protein coat (called the capsid) These are considered non-living b/c they have no metabolism, homeostasis, growth, and require a host cell to carry out their functions Are extraordinarily small, since they are active inside of cel ...
... At their simplest, these are a piece of genetic material with a protein coat (called the capsid) These are considered non-living b/c they have no metabolism, homeostasis, growth, and require a host cell to carry out their functions Are extraordinarily small, since they are active inside of cel ...