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Non-invasive prenatal assessment of trisomy 21 by multiplexed
Non-invasive prenatal assessment of trisomy 21 by multiplexed

... centile of the reference sample set for a one tailed distribution) as the cut-off value to determine if the percentage chromosome 21 was increased and hence fetal trisomy 21 was present. All members of the sequencing and bioinformatics teams were blinded to the karyotype information. Disease classif ...
Expansion of tandem repeats and oligomer
Expansion of tandem repeats and oligomer

... SSR are of considerable practical and theoretical interest due to their high polymorphism [7]. The formation of a hairpin structure during replication [12,13] is believed to be the cause of the CNG repeat expansions, which are associated with a broad variety of genetic diseases. Dimeric SSR of the t ...
Exome sequencing as a tool for Mendelian disease gene discovery
Exome sequencing as a tool for Mendelian disease gene discovery

... of the observed heritability of the trait under study 4,5. Furthermore, little is known about the extent to which rare alleles contribute to the heritability of complex traits6. Since 2005, next-generation DNA sequencing platforms have become widely available, reducing the cost of DNA sequencing by ...
Examination of the molecular control of uterine function
Examination of the molecular control of uterine function

... Do variants of the genes, identified as being differentially expressed between heifers of either high or low fertility occur and are these different in bulls categorised as high and low genetic merit for calving interval, and how are these genetic variants associated with reproductive and other perf ...
mutation - Carol Eunmi LEE - University of Wisconsin–Madison
mutation - Carol Eunmi LEE - University of Wisconsin–Madison

... around longer (when masked in Heterozygote form, not exposed to selection) ...
Reference genome sequence of the model plant Setaria
Reference genome sequence of the model plant Setaria

... Yugu1 mRNA isolated from a variety of different tissues, at different times in development and exposed to different stress treatments, we generated 13 EST libraries (Supplementary Table 2). A total of 63,286 ESTs were sequenced on the Sanger ABI3730xl platform and another 1,217,156 on the 454 FLX pl ...
File
File

... genes in the human genome. What was a typical estimate, based on the results of the Human Genome Project? A. ...
Lecture PPT - Carol Lee Lab - University of Wisconsin
Lecture PPT - Carol Lee Lab - University of Wisconsin

... The generation-time hypothesis. Groups with shorter generations evolve faster because they experience more rounds of germ-cell divisions during an arbitrary unit of time. More rounds of germ-line divisions mean additional DNA synthesis and extra opportunities for mutations that are due to DNA repli ...
Transplantation Immunology pg. 1 Laura Rayne Today I`m going to
Transplantation Immunology pg. 1 Laura Rayne Today I`m going to

... DNA, which is much smaller, circular, and is often found with many copies. These plasmids may contain antibiotic resistance markers. This (the diagram) is an example of the whole genome of m. tuberculosis. Now they have fancy technologies that allow sequencing of an entire genome within a couple of ...
Lecture PPT - Carol Eunmi LEE - University of Wisconsin–Madison
Lecture PPT - Carol Eunmi LEE - University of Wisconsin–Madison

... • Generation-time hypothesis. Groups with shorter generations evolve faster because they experience more rounds of germ-cell divisions during an arbitrary unit of time. More rounds of germ-line divisions mean additional DNA synthesis and extra opportunities for mutations that are due to DNA replicat ...
and interferon-inducible bovine Mx1 promoter
and interferon-inducible bovine Mx1 promoter

... production by Madin-Darby bovine kidney cells (MDBK) were sought after stimulation with a panel of cytokines, LPS, poly-I/C and bovine parainfluenza-3 virus. From the results obtained, it is concluded that the promoter of the bovine MX1 protein shares the basic characteristics found in other species ...
Genomic disorders: structural features of the genome can lead to
Genomic disorders: structural features of the genome can lead to

Identification of a 5S rDNA spacer type specific to Triticum urartu and
Identification of a 5S rDNA spacer type specific to Triticum urartu and

... and C amplify sequences from the short arm of chromosome 1A in ‘Chinese Spring,’ demonstrating the existence of an rDNA locus on chromosome 1A in cultivated wheats. The consistency of the sizes of the products suggests that this sequence type is a recent apomorphism. The results support the proposal ...
The types of muscular dystrophy
The types of muscular dystrophy

... first step: the DNA is denatured and incubated overnight with a mixture of MLPA probes MLPA probes consist of two separate oligonucleotides, each containing one of the PCR primer sequences The two probe oligonucleotides hybridize to immediately adjacent target sequences Only when the two probe oligo ...
If there is time OR when we get to Cell Unit…
If there is time OR when we get to Cell Unit…

... Analogy: ...
Transposable elements, genes and recombination in a 215
Transposable elements, genes and recombination in a 215

... a genome designated Am that is closely related to the genome of T. urartu, the A genome donor for tetraploid and hexaploid wheats. The large genome of T. monococcum (1C =5600 Mb; Bennett and Leitch 1995) is approximately 12 times larger than the genome of rice and 40 times larger than the genome of ...
MultipleSequenceAlignment
MultipleSequenceAlignment

... (Slides by J. Burg) ...
chapter 15 section 3 notes
chapter 15 section 3 notes

Genetic Control of Cell Function and Inheritance
Genetic Control of Cell Function and Inheritance

... composed of the structural proteins and enzymes needed for protein synthesis. As with the other types of RNA, rRNA is synthesized in the nucleus. Unlike other RNAs, ribosomal RNA is produced in a specialized nuclear structure called the nucleolus. The formed rRNA combines with ribosomal proteins in ...
DNA chips
DNA chips

... Readout using micro-flow cells or optic fiber arrays ...
VHA_Genetics_Core _Little_Rock
VHA_Genetics_Core _Little_Rock

... Lateral Sclerosis (ALS) ...
Genetic analysis of acidocin B, a novel bacteriocin
Genetic analysis of acidocin B, a novel bacteriocin

... play an important role in this respect (Gilliland, 1985). The formation of copious amounts of acidic end-products, primarily lactic acid, from the fermentation of carbohydrate-rich substrates such as grain, silage, milk and vegetables is largely responsible for creating an environment unfavourable f ...
HTSanalyzeR - Florian Markowetz
HTSanalyzeR - Florian Markowetz

... Associate Editor: XXXXXXX ...
Down Syndrome: Antonarakis et al. (2004)
Down Syndrome: Antonarakis et al. (2004)

... We might consider that there are two categories of genes on human chromosome 21 (HSA21); those that are dosage sensitive (that is, three copies result in phenotypic effects; shown in red) and contribute to the phenotypes of Down syndrome (DS), and those that are not dosage sensitive (green) and ther ...
Sequence variation: Looking for effects of
Sequence variation: Looking for effects of

... Drosophila [2]; it is not known whether this is true for the fluctuating fitness model. These considerations raise two questions: Are such patterns general? And how can we test between different possible explanations of the patterns? Surveys of DNA sequence variation are rapidly being extended beyon ...
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Genomics

Genomics is a discipline in genetics that applies recombinant DNA, DNA sequencing methods, and bioinformatics to sequence, assemble, and analyze the function and structure of genomes (the complete set of DNA within a single cell of an organism). Advances in genomics have triggered a revolution in discovery-based research to understand even the most complex biological systems such as the brain. The field includes efforts to determine the entire DNA sequence of organisms and fine-scale genetic mapping. The field also includes studies of intragenomic phenomena such as heterosis, epistasis, pleiotropy and other interactions between loci and alleles within the genome. In contrast, the investigation of the roles and functions of single genes is a primary focus of molecular biology or genetics and is a common topic of modern medical and biological research. Research of single genes does not fall into the definition of genomics unless the aim of this genetic, pathway, and functional information analysis is to elucidate its effect on, place in, and response to the entire genome's networks.
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