The molecular epidemiology of iridovirus in Murray cod
... arbitrarily chosen as a limit. If both primers in the pair had E-values of less than 1.0 for the same non-target DNA, they were excluded. Candidate primers for discriminatory assays, C50/C51 and C82/C83, were tested against DNA from MCIV, DGIV-2004, RSIV and EHNV. Primers (M68/ M69 and M151/M152) kn ...
... arbitrarily chosen as a limit. If both primers in the pair had E-values of less than 1.0 for the same non-target DNA, they were excluded. Candidate primers for discriminatory assays, C50/C51 and C82/C83, were tested against DNA from MCIV, DGIV-2004, RSIV and EHNV. Primers (M68/ M69 and M151/M152) kn ...
Quantitating Maxwell® Extracted DNA Samples Using the
... coats and eye protection when working with these or any chemical reagents. Protocols: Quantus™ Fluorometer Operating Manual #TM396 and QuantiFluor® dsDNA System Technical Manual #TM346 are available at: www.promega.com/protocols/ ...
... coats and eye protection when working with these or any chemical reagents. Protocols: Quantus™ Fluorometer Operating Manual #TM396 and QuantiFluor® dsDNA System Technical Manual #TM346 are available at: www.promega.com/protocols/ ...
Polyploidy and genome evolution in plants
... process has forged modern genomes as small as those of Arabidopsis, or with gene numbers that are far less than that expected by the doubling process per se? The answer appears to be that polyploidy is followed by a genomewide removal of some but not all of the redundant genomic material. Differenti ...
... process has forged modern genomes as small as those of Arabidopsis, or with gene numbers that are far less than that expected by the doubling process per se? The answer appears to be that polyploidy is followed by a genomewide removal of some but not all of the redundant genomic material. Differenti ...
Document
... In this quiescent only the cI gene is transcribed: the phage genome exists in a repressed state. In this repressed state, the phage DNA is called a prophage A prophage don’t have the potential to produce other phages. In most cases the phage DNA actually integrates into the host chromosome and is re ...
... In this quiescent only the cI gene is transcribed: the phage genome exists in a repressed state. In this repressed state, the phage DNA is called a prophage A prophage don’t have the potential to produce other phages. In most cases the phage DNA actually integrates into the host chromosome and is re ...
Historical Development of the Concept of the Gene
... heterozygotes are compared. In the cis-heterozygote the mutations are in the same chromosome, but in the trans-heterozygote they are in homologous chromosomes. Thus, the genotype of the cis-heterozygote is designated as a b/, and that of the trans-heterozygote as a/b. If the cis-heterozygote is ...
... heterozygotes are compared. In the cis-heterozygote the mutations are in the same chromosome, but in the trans-heterozygote they are in homologous chromosomes. Thus, the genotype of the cis-heterozygote is designated as a b/, and that of the trans-heterozygote as a/b. If the cis-heterozygote is ...
A reliable and efficient method for deleting
... P1-derived artificial chromosomes (PACs) and bacterial artificial chromosomes (BACs) have become very useful as tools to study gene expression and regulation in cells and in transgenic mice. They carry large fragments of genomic DNA (≥100 kb) and therefore may contain all of the cis-regulatory eleme ...
... P1-derived artificial chromosomes (PACs) and bacterial artificial chromosomes (BACs) have become very useful as tools to study gene expression and regulation in cells and in transgenic mice. They carry large fragments of genomic DNA (≥100 kb) and therefore may contain all of the cis-regulatory eleme ...
PDF
... by Crick in 1958: ‘Once information has passed into protein, it cannot get out again’. At that time, this idea was not supported by reasonable evidence, but now it can be justified in two ways. First, because the genetic code is degenerate; i.e., several triplets codify for each amino acid, and hence ...
... by Crick in 1958: ‘Once information has passed into protein, it cannot get out again’. At that time, this idea was not supported by reasonable evidence, but now it can be justified in two ways. First, because the genetic code is degenerate; i.e., several triplets codify for each amino acid, and hence ...
et al - International Journal of Systematic and Evolutionary
... these proteins was previously available from only a limited number of actinobacteria, whose genomes have been sequenced. One possible signature for actinobacteria, consisting of a large insert in the 23S rRNA, has previously been described (Roller et al., 1992). However, the validity and specificity ...
... these proteins was previously available from only a limited number of actinobacteria, whose genomes have been sequenced. One possible signature for actinobacteria, consisting of a large insert in the 23S rRNA, has previously been described (Roller et al., 1992). However, the validity and specificity ...
insilico.mutagenesis.help.me.please
... sequences up-and downstream of the gene (or target region: e.g. untranslated intergenic regions like promotors) of interest. Please provide a plain nucleotide sequence. All non-DNA characters (e.g. as in FASTA formatted sequences) will cause an error. The addition of flanking nucleotide sequences is ...
... sequences up-and downstream of the gene (or target region: e.g. untranslated intergenic regions like promotors) of interest. Please provide a plain nucleotide sequence. All non-DNA characters (e.g. as in FASTA formatted sequences) will cause an error. The addition of flanking nucleotide sequences is ...
Am. J. Physiol. 1989, 257, L47
... containing portions of the chromosome. The rationale of this probe hunt was that if a large enough number were isolated, some of these should map between met and D7S8 and thus lie closer to the CF gene. Two clones were identified in this manner by the absence of recombination in informative families ...
... containing portions of the chromosome. The rationale of this probe hunt was that if a large enough number were isolated, some of these should map between met and D7S8 and thus lie closer to the CF gene. Two clones were identified in this manner by the absence of recombination in informative families ...
C2005/F2401 `09
... already there. It doesn’t change the amount of enzyme (Eo) but it does change the properties of the enzyme molecules (turnover number and/ or Km). See class handout 15A. Since enzyme levels remain high, but the amount of synthesis of anthrose is not, then the anthrose (the end product) must be inhib ...
... already there. It doesn’t change the amount of enzyme (Eo) but it does change the properties of the enzyme molecules (turnover number and/ or Km). See class handout 15A. Since enzyme levels remain high, but the amount of synthesis of anthrose is not, then the anthrose (the end product) must be inhib ...
Chapter 4. Studying DNA Learning outcomes 4.1. Enzymes for DNA
... results of a restriction digest are examined 5. Distinguish between blunt- and sticky-end ligation and explain how the efficiency of blunt-end ligation can be increased 6. Give details of the key features of plasmid cloning vectors and describe how these vectors are used in cloning experiments, usin ...
... results of a restriction digest are examined 5. Distinguish between blunt- and sticky-end ligation and explain how the efficiency of blunt-end ligation can be increased 6. Give details of the key features of plasmid cloning vectors and describe how these vectors are used in cloning experiments, usin ...
GcvA, a LysR-type transcriptional regulator protein
... was partially digested with Sau3A to give a random distribution of DNA fragments. Size-fractionated fragments of between 5 and 10 kb were ligated into vector pSU19, previously digested with BamHI and treated with calf intestinal alkaline phosphatase to prevent re-annealing. Recombinant DNA was intro ...
... was partially digested with Sau3A to give a random distribution of DNA fragments. Size-fractionated fragments of between 5 and 10 kb were ligated into vector pSU19, previously digested with BamHI and treated with calf intestinal alkaline phosphatase to prevent re-annealing. Recombinant DNA was intro ...
Lab Recap: Miniprep (MP)
... ● Therefore, you need to isolated the plasmid vectors, otherwise known as the plasmid DNA, so that the sequencing reactions work ...
... ● Therefore, you need to isolated the plasmid vectors, otherwise known as the plasmid DNA, so that the sequencing reactions work ...
Rapporto sulle attività e sui risultati conseguiti dal 2004 al 2006
... correlations linking Antarctic and non-Antarctic organisms, addressed to contribute new insight to the reconstruction of the evolutionary history of the Antarctic life. Nevertheless, each RU did as well pursue its own more specific research lines, including structural and functional studies of stres ...
... correlations linking Antarctic and non-Antarctic organisms, addressed to contribute new insight to the reconstruction of the evolutionary history of the Antarctic life. Nevertheless, each RU did as well pursue its own more specific research lines, including structural and functional studies of stres ...
Lesson 2 - Laboratory of Molecular Modelling
... substitutions The idea: Given an alignment of a large number of closely related sequences we can score the relation between amino acids based on how frequently they substitute each other ...
... substitutions The idea: Given an alignment of a large number of closely related sequences we can score the relation between amino acids based on how frequently they substitute each other ...
Test Information Sheet
... For those individuals with clinical features suggestive of a ZRS-related disorder, using genomic DNA obtained from the submitted biological material, bi-directional sequence of the ZRS region is obtained and analyzed. If no mutation is identified by sequence analysis, deletion/duplication analysis c ...
... For those individuals with clinical features suggestive of a ZRS-related disorder, using genomic DNA obtained from the submitted biological material, bi-directional sequence of the ZRS region is obtained and analyzed. If no mutation is identified by sequence analysis, deletion/duplication analysis c ...
Ali J. Berens
... Investigated genetic mechanisms underpinning caste development across three major social insect lineages (bees, ants, wasps) using comparative transcriptomics Tested nourishment as an environmental factor driving developmental plasticity of wasp castes Compared brain gene expression during sta ...
... Investigated genetic mechanisms underpinning caste development across three major social insect lineages (bees, ants, wasps) using comparative transcriptomics Tested nourishment as an environmental factor driving developmental plasticity of wasp castes Compared brain gene expression during sta ...
Lab 17. Chromosomes and Karyotypes: How Do Two Physically
... during gamete production. Therefore, an egg or a sperm cell only gets one of the two alleles that are present in the somatic cells of the organism. This idea is known as the law of segregation. It was brilliant (or lucky) that Mendel chose plant traits that turned out to have a relatively simple gen ...
... during gamete production. Therefore, an egg or a sperm cell only gets one of the two alleles that are present in the somatic cells of the organism. This idea is known as the law of segregation. It was brilliant (or lucky) that Mendel chose plant traits that turned out to have a relatively simple gen ...
Gene Section IGF2R (insulin-like growth factor 2 receptor) in Oncology and Haematology
... Beyond biochemical and DNA sequence properties, M6P/IGF2R epigenetic traits have been described. In humans, there is a differentially methylated region (DMR) in intron 2 of the gene which is preferentially methylated on the maternally inherited copy of the gene; in addition, the human M6P/IGF2R resi ...
... Beyond biochemical and DNA sequence properties, M6P/IGF2R epigenetic traits have been described. In humans, there is a differentially methylated region (DMR) in intron 2 of the gene which is preferentially methylated on the maternally inherited copy of the gene; in addition, the human M6P/IGF2R resi ...
Document
... Mendel’s cross between tall pea plants yielded all tall pea plants. His cross between small pea plants yielded all small pea plants. ...
... Mendel’s cross between tall pea plants yielded all tall pea plants. His cross between small pea plants yielded all small pea plants. ...
Practice Questions for Ecology
... Keystone Eligible Content: (Just a checklist- do not answer here.) Describe and/or predict observed patterns of inheritance (i.e. dominant, recessive, co-dominance, incomplete dominance, sex-linked, polygenic, and multiple alleles) Describe processes that can alter composition or number of chrom ...
... Keystone Eligible Content: (Just a checklist- do not answer here.) Describe and/or predict observed patterns of inheritance (i.e. dominant, recessive, co-dominance, incomplete dominance, sex-linked, polygenic, and multiple alleles) Describe processes that can alter composition or number of chrom ...