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Genomic Databases for Tomato
Genomic Databases for Tomato

... Abstract Tomato (Solanum lycopersicum) is a model plant of the Solanaceae family. Various biological aspects of tomato have been investigated with molecular biological approaches, and a significant amount of DNA and protein sequencing data on tomato has been accumulated. Recently, the number of toma ...
Microarray statistical validation and functional annotation
Microarray statistical validation and functional annotation

... difficult to role out functional correlations between the differentially expressed genes. If the stringency of the statistical validation is too loose the increase of false positives creates background noise from which is difficult to extract trustful functional correlations between the differential ...
DNA Structure - StudyTime NZ
DNA Structure - StudyTime NZ

... As egg cells are a type of gamete, they will undergo Meiosis. This is because they are only involved in reproduction, and have no need to perform growth or repair. Meiosis involves two cell divisions but only one DNA replication. This results in daughter cells with half the number of chromosomes pre ...
Cloning of PCR products into TOPO TA vectors
Cloning of PCR products into TOPO TA vectors

... The linearised TOPO TA vector, pCR2.1-TOPO is specially designed for cloning such DNA fragments. It has single overhanging deoxythymidine (dT) residues at its 3’ ends adjoining the sequence 5’-CCCT-3’ (see Figure 2). This allows base-pairing of the A and T residues between vector and PCR products. L ...
National Center for Biotechnology Information (NCBI)
National Center for Biotechnology Information (NCBI)

... * = unlimited number of characters The system allows you to do left and right truncation. 5. If wildcards are allowed then what symbols are used? Not available 6. How do you search for a phrase? Enclose your phrase in quotes. 7. Any unique features? Cn3D – software that allows you to see and manipul ...
Binding of ColEl-kan Plasmid DNA by Tobacco
Binding of ColEl-kan Plasmid DNA by Tobacco

... the screening procedure employed required that the kanamycin resistance gene be maintained within the plant cell for many generations. Maintenance via autonomous replication of the plasmid would require that the plasmid be taken up physically intact by the protoplasts. This may not have occurred. Th ...
Concept of DNA and RNA
Concept of DNA and RNA

... been nicknamed the Hershey-Chase Experiment. At that time, people knew that viruses were composed of DNA (or RNA) inside a protein coat/shell called a capsid. It was also known that viruses replicate by taking over the host cell metabolic functions to make more virus. We are used to thinking and tal ...
Full Text
Full Text

... refresh acquaintance with several common terms and basic genetic concepts (a brief glossary is provided in Table 1). Phenotype refers to the observable characteristics (or symptoms of illness) under consideration. Genotype refers to the set of genes an individual possesses that are relevant to the p ...
BioUnit3AlignedMaterialsList
BioUnit3AlignedMaterialsList

... reason for these differences in physical characteristics (called phenotype) is the different combination of genes possessed by each individual. To illustrate the tremendous variety possible when you begin to combine genes, you and a classmate will establish the genotypes for a potential offspring. Y ...
chapter 17 from gene to protein
chapter 17 from gene to protein

... The genetic instructions for a polypeptide chain are written in DNA as a series of nonoverlapping three-nucleotide words. During transcription, one DNA strand, the template strand, provides a template for ordering the sequence of nucleotides in an RNA transcript.  A given DNA strand can be the temp ...
Genes can be switched on and off by the protein CTCF
Genes can be switched on and off by the protein CTCF

... I injected DNA into mouse embryos, using a special reporting system to see if CTCF was active or not. Dependent upon whether the DNA contained a binding site for CTCF so that CTCF could bind and function, or contained no such binding site, different signals were expected. However, I could not observ ...
Question 2 (cont.) - Amazon Web Services
Question 2 (cont.) - Amazon Web Services

... DeSTRLab consists of a simple laboratory process management capability and the expert modules for DNA analysis work. The goal is to replace the process laboratory process management capability of DeSTRLab with that of Labware, while retaining and enhancing the processing throughput of the DNA expert ...
Medical Genetics
Medical Genetics

... normal DNA and package it into a vector, a molecular delivery truck usually made from a disabled virus. Doctors then infect a target cell —usually from a tissue affected by the illness, such as liver or lung cells—with the vector. The vector unloads its DNA cargo, which then begins producing the mis ...
Andrew Farmer NCGR Paul Scott Iowa State Bill
Andrew Farmer NCGR Paul Scott Iowa State Bill

... Sampling ...
Candidatus Paenicardinium endonii
Candidatus Paenicardinium endonii

... International Journal of Systematic and Evolutionary Microbiology 56 IP: 88.99.165.207 On: Fri, 16 Jun 2017 00:15:40 ...
- Wiley Online Library
- Wiley Online Library

... requires genetic variation upon which to act. This fundamental neoDarwinian notion permeates all of biological thought as well as biology education. Probably, most biologists assume that the power and primacy of natural selection is dependent on the existence, amount, and structuring of standing gen ...
Nucleotide sequences from tomato leaf curl viruses from different
Nucleotide sequences from tomato leaf curl viruses from different

... that found in other W T G s from the Old World; most of the sequence that is repeated also occurs in TYLCV-ISR, T L C V - A and a Brazilian form of B G M V (ArgfielloAstorga et al., 1994). The comparable repeats in TYLCVSAR and T L C V - I N differ to a greater extent from those in ITmLCV. Analysis ...
Modeling Spatial Correlation of DNA Deformation
Modeling Spatial Correlation of DNA Deformation

... experiment with lP ∼ 150 bps ∼ 50 nm for double-stranded DNA under physiological conditions18 as well as in a flow field.19 Detailed variations of this model have been proposed over the years by introducing a small number of additional independent parameters,20 such as the twisting persistence length, ...
Expressed Sequence Tag (EST)
Expressed Sequence Tag (EST)

A Variant within the DNA Repair Gene XRCC3 Is
A Variant within the DNA Repair Gene XRCC3 Is

... involved in repairing UV-DNA damage are the base excision, the nucleotide excision, and the homologous double-stranded DNA repair pathways. This study used a sequence-specific primer PCR (PCR-SSP) genotyping method to investigate the association between polymorphisms in DNA repair genes from these p ...
Structure of B-DNA with Cations Tethered in the Major Groove†
Structure of B-DNA with Cations Tethered in the Major Groove†

... DNA Conformation. The final model of DDD4+, including the amino-propyl groups, is well-determined by the data (Figure 2). The occupancies of the two terminal O5′ atoms of the DNA were set to zero because they appear to be disordered; there is no electron density observed around them. Each of the fou ...
Retrieving data from UniProt databases Further reading Support
Retrieving data from UniProt databases Further reading Support

... Why do we need UniProt? We can find out much more about the function of proteins by studying properties such as the biological processes they are involved in, their post-translational modifications, their interaction with other molecules, and their location in cells and organisms, than we could ever ...
Gene List Enrichment Analysis
Gene List Enrichment Analysis

Dravets_LETM1 - Medicinal Genomics
Dravets_LETM1 - Medicinal Genomics

... anchor proteins, might be responsible. Recent studies on animal models showed that alterations in the biogenesis of GPI anchor proteins can alter expression of Nav1.1 encoded by SCN1A [21] gene. As epilepsy is such a variable disorder that can present itself in different types and different phenotyp ...
Quantitating Maxwell® Extracted DNA Samples Using the
Quantitating Maxwell® Extracted DNA Samples Using the

... coats and eye protection when working with these or any chemical reagents. Protocols: Quantus™ Fluorometer Operating Manual #TM396 and QuantiFluor® dsDNA System Technical Manual #TM346 are available at: www.promega.com/protocols/ ...
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Genomics

Genomics is a discipline in genetics that applies recombinant DNA, DNA sequencing methods, and bioinformatics to sequence, assemble, and analyze the function and structure of genomes (the complete set of DNA within a single cell of an organism). Advances in genomics have triggered a revolution in discovery-based research to understand even the most complex biological systems such as the brain. The field includes efforts to determine the entire DNA sequence of organisms and fine-scale genetic mapping. The field also includes studies of intragenomic phenomena such as heterosis, epistasis, pleiotropy and other interactions between loci and alleles within the genome. In contrast, the investigation of the roles and functions of single genes is a primary focus of molecular biology or genetics and is a common topic of modern medical and biological research. Research of single genes does not fall into the definition of genomics unless the aim of this genetic, pathway, and functional information analysis is to elucidate its effect on, place in, and response to the entire genome's networks.
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