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2003 ARS Immunology Research Workshop
2003 ARS Immunology Research Workshop

... On 12-14 December, 2006, more than eighty scientists from the European Union and from the United States, representing the fields of immunology and vaccinology, gathered at the National Animal Disease Center (NADC) in Ames, Iowa, to discuss, design and prioritize initiatives for vaccine research. The ...
Kuby`s Immunology
Kuby`s Immunology

... animal and a bone marrow chimera animal?  What is the importance of a bone marrow chimera animal in immunological studies?  Explain how the control of MHC restriction is the genetic basis for the functional specificity of T cells. ...
fmd with viaa test incl.
fmd with viaa test incl.

... been adapted for the detection of both AHS virus (AHSV) and specific antibodies. Identification of the agent: It is particularly important to perform virus isolation and serotyping whenever AHS outbreaks occur outside the enzootic regions in order to choose a homologous serotype for the vaccine. AHS ...
File - wilson science WEBSITE
File - wilson science WEBSITE

... • The five major classes of antibodies, or immunoglobulins, differ in distribution and function • Polyclonal antibodies are the products of many different clones of B cells following exposure to a microbial antigen • Monoclonal antibodies are prepared from a single clone of B cells grown in culture ...
Neisseria gonorrhoeae Variation of Lipooligosaccharide Directs Dendritic Cell–Induced T Helper Responses
Neisseria gonorrhoeae Variation of Lipooligosaccharide Directs Dendritic Cell–Induced T Helper Responses

... and female genital tissues If the C-type lectins MGL and DC-SIGN are physiological important receptors that play an active role in the pathogenesis of GC, these proteins should be expressed at the site of GC entry, the female cervix and the male penile urethral tissue. DC-SIGNpos cells have been sho ...
Transformation Lab - Towson University
Transformation Lab - Towson University

... transformation, bacteria take up exogenous (foreign) DNA and produce the genetic products (proteins) encoded in the foreign DNA. Transformation enables inexpensive and reliable production of important medical products such as insulin, human growth hormone, and other replacement hormone and gene ther ...
Psychoneuroimmunology: Psychological Influences
Psychoneuroimmunology: Psychological Influences

... example, higher NK cell activity and stronger proliferative responses of peripheral blood leukocytes to mitogen stimulation were associated with higher social support in women whose husbands were being treated for urologic cancer than among those with less support (Baron, Cutrona, Hicklin, Russell, ...
NFkB/Rel familien
NFkB/Rel familien

... Signaling induction of cyclin D1. Two signalling pathways contribute to the induction of cyclin D1 transcription in mammary epithelial cells.  One pathway, which leads to activation of transcription factor AP1, is activated by growth factors (GF), which bind to receptor tyrosine kinases (RTK). This ...
A simple set of rules for primer sequence design is as follows
A simple set of rules for primer sequence design is as follows

... sequester the cation; of these, dNTPs are the most concentrated, so [Mg2+] should be 0.5 2.5mM greater than [dNTP]. A titration should be performed with varying [Mg2+] with ...
Licentiate thesis from Department of Molecular Biosciences, The
Licentiate thesis from Department of Molecular Biosciences, The

... intersection between the innate and the adaptive arm, as they share features from both the innate and adaptive immune cells. Unconventional T cells are further subdivided into mucosaassociated invariant T (MAIT) cells, γδ T cells and NKT cells (16). Little is known about MAIT cells, but along γδ T c ...
Virginia State Science Olympiad Regional Tournament
Virginia State Science Olympiad Regional Tournament

... a. Mitosis results in four haploid daughter cells, while meiosis results in two diploid daughter cells. b. DNA synthesis only occurs once before either mitosis or meiosis. c. During anaphase I, homologous pairs of chromatids are separated; during regular anaphase, homologous pairs of chromosomes are ...
PDF
PDF

... that best activate the immune response are usually selected. An influenza vaccine in the form of shots is an example. In addition, a recombinant subunit vaccine has been made for the hepatitis B virus. Hepatitis B genes are injected into maker cells in culture. Once these cells reproduce, the desire ...
Gene Section RAD52 (RAD52 homolog (S. cerevisiae)) Atlas of Genetics and Cytogenetics
Gene Section RAD52 (RAD52 homolog (S. cerevisiae)) Atlas of Genetics and Cytogenetics

... the Saccharomyces cerevisiae RAD52 protein (ScRAD52) both structurally and biochemically. However the phenotypic properties of RAD52, ...
PPT - Altogen Biosystems
PPT - Altogen Biosystems

RNA-Mediated Programming of Developmental
RNA-Mediated Programming of Developmental

... autogamy were carried out at 27°C as previously described (26). The expressed surface antigen was determined by adding serotype-specific antisera (1:200 to 1:1,000) to small volumes of medium in depression slides. Cells expressing the corresponding antigen were immobilized within 1 h at 27°C. Constr ...
Chromosome-encoded gene cluster for the
Chromosome-encoded gene cluster for the

... gene clusters are very similar in their genetic organization; both contain genes encoding a multi-component aniline dioxygenase (AD), a LysR-type regulator, and several metacleavage pathway enzymes. The ADs encoded by these gene clusters consist of five proteins, two of which are homologous to gluta ...
2008 HSC Notes from the Marking Centre Biology
2008 HSC Notes from the Marking Centre Biology

... set up correctly for the dependent and independent variable, and correctly plotted points. In the weaker responses, candidates had an inappropriate scale (ie not multiples of 2, 5 or 10) making it difficult to plot their points accurately. Some weaker responses produced poor quality graphs that were ...
1 INTRODUCTION TO GLOMERULAR DISEASES Goal: understand
1 INTRODUCTION TO GLOMERULAR DISEASES Goal: understand

... border. Animals develop antibodies against the brush border antigen. These antibodies also react with the basal surface of epithelial cells. Complement activation ensues and shedding of the immune complexes from cell surface to sub-epithelial location occurs and granular sub-epithelial deposits are ...
Genetic Markers for Sex Identification in Forensic DNA Analysis
Genetic Markers for Sex Identification in Forensic DNA Analysis

... casework. For a DNA profile to be accepted into the US National DNA Index System (NDIS), which is the national level of the Combined DNA Index System (CODIS), inclusion of the amelogenin marker is required for relatives of missing persons, along with the 13 CODIS Core Loci [1]. The amelogenin test m ...
Physical map of the aromatic amine and m-toluate
Physical map of the aromatic amine and m-toluate

... present in Pseudomonasputida UCC22, a derivativeof P.putida mt-2. The plasmid is 79 1kbp in size and can be divided into a restriction-site-deficient region of 51 f 1 kbp and a restriction-site-profuse region of 28 kbp which begins and ends with directly repeating sequences of at least 2 kbp in leng ...
1 - life.illinois.edu
1 - life.illinois.edu

... mutation in the dnaA gene (dnaATS), You have isolated a Tn10 insertion (encodes resistance to tetracycline) that is 25 % linked to the dnaATS mutation by P22 HT mediated transduction. a. How could you transfer the dnaATS mutation to a new S. typhimurium strain? How would you show that the new strain ...
Preparation of PCR Products for DNA Sequencing
Preparation of PCR Products for DNA Sequencing

... suboptimal PCR were revealed by DNA sequencing and autoradiographic detection (n >10; data not shown). Artifacts ranged from 52–68 bp in length. A strong interfering signal was observed across all four lanes at a position corresponding to a product length of 52 nucleotide (nt) on the sequencing gels ...
LESSON 4 Using Bioinformatics to Analyze Protein
LESSON 4 Using Bioinformatics to Analyze Protein

... the start codon, AUG (circled). In the DNA, this sequence is ATG (also circled). When genetic researchers study genes, they often do not write down the complementary DNA sequence, the mRNA sequence, and the tRNAs. They use a “short cut.” Because the coding sequence of the DNA and the sequence of the ...
Explaining the Likelihood Ratio in DNA Mixture
Explaining the Likelihood Ratio in DNA Mixture

... single number the data support for a hypothesis. It is a way of accounting for all the evidence in favor of or against a particular hypothesis (or proposition) (1). The LR is also the match statistic that is used in DNA reporting (2-4). The LR's good legal and scientific standing underlies forensic ...
The role of lysosomal cysteine proteases in crustacean immune
The role of lysosomal cysteine proteases in crustacean immune

... ability in invertebrates to produce immune cells specially designed to attack a specific antigen in a long term response, also called “immunological memory”, whose main purpose is to protect the host from re-infections and protect the immunologically immature offspring (Welsh et al., 2004). To date, ...
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DNA vaccination



DNA vaccination is a technique for protecting an animal against disease by injecting it with genetically engineered DNA so cells directly produce an antigen, resulting in a protective immunological response. Several DNA vaccines have been released for veterinary use, and there has been promising research using the vaccines for viral, bacterial and parasitic diseases, as well as to several tumour types. Although only one DNA vaccine has been approved for human use, DNA vaccines may have a number of potential advantages over conventional vaccines, including the ability to induce a wider range of immune response types.
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