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2054, Chap. 13, page 1 I. Microbial Recombination and Plasmids
2054, Chap. 13, page 1 I. Microbial Recombination and Plasmids

... I. Microbial Recombination and Plasmids (Chapter 13) A. recombination = process of combining genetic material from 2 organisms to produce a genotype different from either parent (exchange of DNA between different genes) 1. occurs during meiosis as crossing over between homologous chromosomes 2. gene ...
Activity--Extracting DNA - e
Activity--Extracting DNA - e

... In the Space Station Alpha mission preparation, your students learned that the solar storm represents a great danger to the astronauts if they are not shielded from the damaging radiation of the sun. The solar rays can penetrate the layers of the space station and damage the astronauts’ DNA, the gen ...
Understanding the Immune System
Understanding the Immune System

... – T-Cells (Thymus derived) Natural Killer Cells (Innate Immunity)  CD4+ T-Cells (helper cells)  CD8+ T-Cells (cytotoxic cells) ...
Making Recombinant DNA
Making Recombinant DNA

... recombinant DNA. There are several ways of joining the donor to the vector to create a recombinant DNA molecule. Cleave DNA at a specific sequence and make single-stranded sticky tails. Such strands in the donor DNA then anneal to sticky ends in the vector, which has been cleaved by the same restric ...
Vaccines: Fact and Fiction - Voelcker Biosciences Teacher Academy
Vaccines: Fact and Fiction - Voelcker Biosciences Teacher Academy

... C. The 2014 vaccination schedule protects against more pathogens than the 1980 vaccination schedule. ...
Principles of immunodetection
Principles of immunodetection

... Involved in allergic responses ...
PCR
PCR

... 1) Bacterial cells may not be able to use a eukaryote’s gene since they often use different enzymes Eukaryotes can be used as hosts, and yeast does quite well. It is very difficult to get plant/animal cells to take up foreign DNA ...
2. recombinant gene
2. recombinant gene

... 2. Ca-phosphate 3. Electroporation 4. Gene gun ...
Biotechnology
Biotechnology

... ends” of the two DNA pieces together with DNA ligase (enzyme) – also known as gene splicing.  This creates a vector = a DNA molecule used to carry a gene of interest from one organism to another. ...
Chapter 19 (part 2) - Nevada Agricultural Experiment
Chapter 19 (part 2) - Nevada Agricultural Experiment

... • In duplex DNA, ten bp per turn of helix (relaxed form) • DNA helix can be over-wound. • Over winding of DNA helix can be compensated by supercoiling. • Supercoiling prevalent in circular DNA molecules and within local regions of long linear DNA strands • Enzymes called topoisomerases or gyrases ca ...
1. dia
1. dia

... 2. Ca-phosphate 3. Electroporation 4. Gene gun ...
The Cell
The Cell

... bacteria) with no nucleus-just loop of DNA, ribosomes and a membrane, but no membrane bound organelles ...
S1.Describe how the tight packing of chromatin in a closed
S1.Describe how the tight packing of chromatin in a closed

... the ferritin mRNA to be translated, producing more ferritin protein. IREs are also located in the transferrin receptor mRNA in the 3′-UTR. When IRP binds to these IREs, it increases the stability of the mRNA. This leads to an increase in the amount of transferrin receptor mRNA within the cell when t ...
Antibodies
Antibodies

... 2. Macrophages engulf the microbes by phagocytosis • Digested microbes enter the lymph and are destroyed. • Ab can cause microbes to clump making them ...
Document
Document

... the ferritin mRNA to be translated, producing more ferritin protein. IREs are also located in the transferrin receptor mRNA in the 3-UTR. When IRP binds to these IREs, it increases the stability of the mRNA. This leads to an increase in the amount of transferrin receptor mRNA within the cell when t ...
RNA and Protein Synthesis
RNA and Protein Synthesis

... If a strand of DNA says this: T C G G A C T A A A G C C U G A U U What will the strand of mRNA say? – Congratulations! You just did a process called transcription ! ...
Plasmid
Plasmid

...  found mostly in bacterial cells  carry genes that benefit the survival of the organism (antibiotic resistance). ...
Genomes 1
Genomes 1

... To extract the required gene from an organism or manufacture a copy of the gene A way of getting it into the organism you want to genetically modify To have the gene replicated and expressed in the modified organism ...
Biotechnology 1
Biotechnology 1

...  Bacteria are opportunists ...
Biotechnology_S14
Biotechnology_S14

... 1. DNA from blood or other tissues is placed into a tube. Restriction • Restriction enzymes cut up DNA. If enzymes are also added to the tube. the DNA wasn’t cut up then there  Why do we add restriction enzymes to the DNA?  Do we add the same or different restriction enzymes to each setup? Why? 2. ...
Southern Transfer
Southern Transfer

... Probes will be hybridized to genomic DNA on the nylon membrane to determine which restriction fragment(s) may harbor the Myb ...
Chapter 09 Lecture PowerPoint - McGraw Hill Higher Education
Chapter 09 Lecture PowerPoint - McGraw Hill Higher Education

... • Cro and l repressor share affinity for the same operators, but have microspecificities for OR1 or OR3 • These specificities are determined by interactions between different amino acids in the recognition helices of the 2 proteins and different base pairs in the 2 operators ...
Study guide unit 3
Study guide unit 3

... 2. What are the characteristics of arthropods? Provide examples. 3. What are the characteristics of insects? Provide examples. 4. What are the 4 stages of insect metamorphosis? 5. How are maggots used to determine the post mortem interval? 6. What types of insects feed on a corpse? 7. How do weather ...
Basic Immunology
Basic Immunology

antigens
antigens

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DNA vaccination



DNA vaccination is a technique for protecting an animal against disease by injecting it with genetically engineered DNA so cells directly produce an antigen, resulting in a protective immunological response. Several DNA vaccines have been released for veterinary use, and there has been promising research using the vaccines for viral, bacterial and parasitic diseases, as well as to several tumour types. Although only one DNA vaccine has been approved for human use, DNA vaccines may have a number of potential advantages over conventional vaccines, including the ability to induce a wider range of immune response types.
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