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Notes to Educators
Notes to Educators

... • Protein sequences are numbered from the N (amino) terminus to the C (carboxy) terminus, beginning with 1. Since all proteins start by reading the AUG codon, all proteins are made with methionine at the beginning, which is numbered 1. But if you looked at a number of protein sequences, you might di ...
Genetic Continuity
Genetic Continuity

... ◦ Locate and decode genes that cause diseases  We could then develop ways to treat the disease ...
HIV Vaccine
HIV Vaccine

... Carrying antigens A and B Molecular Biology of the Cell Alberts et al ...
AIDS Vaccine
AIDS Vaccine

... Carrying antigens A and B Molecular Biology of the Cell Alberts et al ...
Causes of cancer
Causes of cancer

... 3. Benzo[a]pyrene-7, 8-dihydrodiol is further metabolized at the olefinic double bond by cytochrome P450 to form a vicinal diol-epoxide (r7, t8-dihydroxy-c9, 10 epoxy-7,8,9,10tetrahydroxybenzo[a]pyrene). 4. The highly unstable arene ring opens spontaneously to form a carbocation. 5. This electrophil ...
Chapter 17 - HCC Learning Web
Chapter 17 - HCC Learning Web

... A) attachment of amino acids to tRNAs. B) binding of ribosomes to mRNA. C) shape of the A and P sites of ribosomes. D) bonding of the anticodon to the codon. E) bonding of the anticodon to the codon and the attachment of amino acids to tRNAs. 10) What is the effect of a nonsense mutation in a gene? ...
Genetics and Heredity
Genetics and Heredity

... European descent but is much rarer in other groups. One out of 25 whites (4% ) is a carrier. The normal allele for this gene codes for a membrane protein that functions in chloride ion transport between certain cells and the extracellular fluid. These chloride channels are defective or absent. The r ...
Modern Genetics Meets the Dodo and the Solitaire
Modern Genetics Meets the Dodo and the Solitaire

... d. ribosomes 6. What happens during replication? a. DNA is copied. b. RNA is copied. c. Ribosomes are made. d. Proteins are made. 7. Which base is found only in RNA? a. thymine ...
DNA replication
DNA replication

... Characteristic of DNA 1. carry information from one generation to the next. 2.puts that information to work by determining the inheritable characteristics of an organism. 3. has to be easily copied since all the DNA is replicated each time a cell divides. ...
Immunology: Basic Principles of Adaptive Immunity and Immunizations
Immunology: Basic Principles of Adaptive Immunity and Immunizations

... makes contact with the T helper cell and the T helper then produces lymphokines that stimulate thethe B cell to differentiate ...
Nucleotide is composed of a ribose sugar, a base and a phosphate
Nucleotide is composed of a ribose sugar, a base and a phosphate

... DNA strands: The start of the gene is always closest to the 5' end • Template strand/ non-coding strand/ antisense (3' to 5') strand – The template used for transcription • Non-template strand/ coding strand/ sense (5' to 3') strand – Codes for the gene. Same sequence as the new RNA strand (T replac ...
AP Biology Ch. 20 - apbiologyclass / FrontPage
AP Biology Ch. 20 - apbiologyclass / FrontPage

... • Automation has allowed scientists to measure expression of thousands of genes at one time using DNA microarray assays • DNA microarray assays compare patterns of gene expression in different tissues, at different times, or under different conditions ...
Biotechnology and Recombinant DNA I. Tools of Biotechnology
Biotechnology and Recombinant DNA I. Tools of Biotechnology

... portion of the Ti plasmid (T-DNA) is transferred into the plant cell. • This system has been well characterized and is now used to introduce foreign DNA into plants as well as some animal cells. iv. Screening or Detection of Recombinant Molecules • May be creating a scenario not much different than ...
Slide 1
Slide 1

... Describe antibodies (ab) and antigens (ag) and how they work.  Purify IgY antibodies from egg yolk and identify using the dot-blot method of immunodetection.  Describe immune response and relate to vaccination.  Explain how principles of immunology are used in clinical diagnostics. ...
Supplementary Methods
Supplementary Methods

... All yeast strains were congenic with the MHY501 wild-type (see ref. 4) except where noted. Strains in which various chromosomal genes were tagged in frame with the DNA sequence for enhanced green fluorescent protein (GFP) were constructed by single-step PCR amplification of the terminal copy of the ...
lec36_2013 - Andrew.cmu.edu
lec36_2013 - Andrew.cmu.edu

... widely used bacteria) using recombinant DNA methods. The structure of the purified protein can be determined and new HIV protease inhibitors can be designed that will be effective against the altered virus. The overall procedure is as follows: 1) Isolate the genetic material (RNA) from the HIV virus ...
Lab 8
Lab 8

... Most cells in our body contain DNA, the molecule that makes up the genes that control our physical characteristics. Half your DNA came from your mother, and half from your father. Your DNA is what determines your eye color, hair color, height, blood type, and all other physical properties of your bo ...
Quiz 22
Quiz 22

... A. (i)  (iv)  (ii)  (iii) B. (iii)  (ii)  (iv)  (i) C. (iii)  (iv)  (ii)  (i) D. (iv)  (ii)  (iii)  (i) 9. Both genetic engineering and selective breeding can be used to produce organisms with desirable characters. Which of the following are the advantages of genetic engineering over sel ...
Genotyping of Transgenic Mice Population
Genotyping of Transgenic Mice Population

... buffer should cover the gel slightly DNA Prep 1. To your amplified DNA sample, add loading dye in appropriate volume; add 4 μLof 6x Loading Dye 2. Mix DNA and dye well 3. Add about 10 μL DNA to each well 4. In addition to DNA add 3-4 μL DNA ladder to one of the wells 5. Run the gel at around 100 v f ...
20.1 Structural Genomics Determines the DNA Sequences of Entire
20.1 Structural Genomics Determines the DNA Sequences of Entire

... b. Orthologs are more similar than paralogs. c. Orthologs are in the same organism; paralogs are in different organisms. d. Orthologs are in different organisms; paralogs are in the same organism. ...
Chap9 Immunotherapy
Chap9 Immunotherapy

... To avoid the generation of escape variants in solid tumors, immune response against a broad spectrum of tumor-derived epitopes is highly desirable. ...
cytotoxic T cell
cytotoxic T cell

endocrine system - Crestwood Local Schools
endocrine system - Crestwood Local Schools

幻灯片 1 - TUST
幻灯片 1 - TUST

... Frequently Gene-specific probes are constructed with cDNA clones. If the gene of interest is expressed in a specific tissue or cell type, its mRNA is often relatively abundant. Although mRNA is not available in sufficient quantity to serve as a probe, the desired mRNA species can be converted into c ...
Host Defenses Immune System Terminology White Blood Cells
Host Defenses Immune System Terminology White Blood Cells

... Involves Ab produced by B cells Cell-mediated immunity Involves T cells Acquired immunity Developed during an individual's lifetime •Naturally acquired active immunity (Resulting from infection) •Naturally acquired passive immunity (Transplacental or via colostrum) •Artificially acquired active immu ...
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DNA vaccination



DNA vaccination is a technique for protecting an animal against disease by injecting it with genetically engineered DNA so cells directly produce an antigen, resulting in a protective immunological response. Several DNA vaccines have been released for veterinary use, and there has been promising research using the vaccines for viral, bacterial and parasitic diseases, as well as to several tumour types. Although only one DNA vaccine has been approved for human use, DNA vaccines may have a number of potential advantages over conventional vaccines, including the ability to induce a wider range of immune response types.
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