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Take-Home Exam 1
Take-Home Exam 1

... CF gene? Describe how a lambda phage or cosmid genomic library, such as those used in these studies might be constructed. d. What is chromosome walking? How does it differ from the new method Collins developed, called chromosome jumping? e. The regions of the CF gene that code for protein were ident ...
Tumor antigens
Tumor antigens

... formation are distinctions that immune cells can use to recognize tumor cells. Many of these mutations have been proved to be tumor antigens. • B cell tumor antigens: Serum from cancer patients were used to clone tumor antigens from cDNA library prepared from tumor cells (SEREX). Many of these antig ...
Document
Document

... LINE DNA transcribed. mRNA exported from nucleus. ORF1 and ORF2 proteins are translated from mRNA, remain attached to 3’-end of transcript, and transport mRNA back into the nucleus where it associates with T- rich DNA by use of the poly-A tail To form a DNA:RNA hybrid. ORF2 (RT and endonuclease) nic ...
DNA - Madison Public Schools
DNA - Madison Public Schools

... to 7 repeating base pairs. STR’s are less susceptible to degradation and can be amplified by PCR Multiplexing: A technique that ...
Academic Biology
Academic Biology

... Genetic terms ( give Examples) o Heterozygous o Homozygous o Hybrid o Allele o Trait o Phenotype o Genotype ...
Chapter 8: Recombinant DNA Technology 1. Tools of Recombinant
Chapter 8: Recombinant DNA Technology 1. Tools of Recombinant

... Harvest copies of gene to insert into plants or animals ...
Practice EOC Questions
Practice EOC Questions

... A. It maintains the same exact DNA from one generation to the next. B. It helps to increase genetic variation. C. It promotes more interaction between males and females of the same species. D. It helps maintain the chromosome number of the species. The correct answer is… B ...
Gene Cloning
Gene Cloning

... translation initiation codon (normally ATG)and the translation termination codon (TAA, TGA or TAG) It is important that you appreciate the difference between a gene and a coding region. In many genetic engineering experiments we will wish to express a protein and so will only be interested in the co ...
Graduate School of Public Health
Graduate School of Public Health

... increased reaching almost 80% with an estimated three million childhood deaths prevented each year. Yet infectious diseases are still responsible for a third of all deaths, killing at least 13 million people a year. Of those, more than 5 million are children under five. There are, however, reasons t ...
Name: Date: Period: _____ The Immune Response: Web Analysis
Name: Date: Period: _____ The Immune Response: Web Analysis

... 4. Dendritic cells (aka APC’s) present antigens to helper T cells  specific immune response 1. nonspecific defenses come first (ex: skin, enzymes in mucous membranes, macrophages) 2. once past the nonspecific defenses, pathogens travel through the blood 3. If antibodies are present, they can attach ...
Document
Document

... from the V region genes • Multiple choices of V region genes available • A mechanism to rearrange V and C genes in the genome so that they can fuse to form a complete Immunoglobulin gene. ...
幻灯片 1
幻灯片 1

... TI-1 antigen: also named B cell mitogen 1. Contains B cell mitogen and repeated B epitopes 2. Can induce immature and mature B cell-mediate immune response 3. In high concentrations, can induce polyclonal B-cell activation 4. In lower concentrations, only the BCR-binding TI-1 antigens can activate ...
Microbiology – Chapter 15
Microbiology – Chapter 15

... 6. Small molecules that are too small to cause an immune response are called haptens. Penicillin is an example. By itself, too small to be antigenic, but it combines with serum proteins and then can become antigenic (penicillin allergy ) ...
Immunopathology I
Immunopathology I

... macrophages digest it and make it into amino acids that are no longer stimulatory for the T-cell, then the whole response comes down). If the antigen is hard for the macrophages to digest (like fungal cell walls, for example), then the macrophages can’t completely clear the material, the T-cells con ...
cells
cells

... • One from father, one from mother • All body cells Haploid • 1 of each type of chromosome • Only in gametes (reproductive cells) Aneuploid • Wrong number of chromosomes • Extra or missing chromosomes ...
DNA - Midlakes
DNA - Midlakes

... If I were to take apart several boats and line up all of the pieces for comparison. Then I compare the relative size of the rudders, bows, and decks, I could tell that two sail boats are more closely related to each other than to a battleship, based on size. Gel Electrophoresis helps to make similar ...
Chapter 10: Retroelements in the Mouse
Chapter 10: Retroelements in the Mouse

... __________________ are large numbers of non-autonomous retroelements that encode no proteins and are dependent on the reverse transcriptase-competent autonomous retroelements for their mobility. Which of the following have open reading frames that do not encode proteins but are framed by LTR? a. SIN ...
Zinc finger nucleases
Zinc finger nucleases

... Meganucleases • Meganucleases, found commonly in microbial species, have the unique property of having very long recognition sequences (>14bp) thus making them naturally very specific. ...
Introduction to Immunology and Immunotoxicology
Introduction to Immunology and Immunotoxicology

... Such an approach allows for comparisons of different studies on the same test substance and for comparisons of conclusions across studies, to ensure similar criteria are employed uniformly The NTP has developed guidance notes as to how these criteria should ...
Activity 3.1.4 - Central Magnet School
Activity 3.1.4 - Central Magnet School

... Isolate mRNA from normal and ...
PowerPoint Presentation - I. Introduction to class
PowerPoint Presentation - I. Introduction to class

... Adaptive (acquired) immunity Refers to antigen-specific defense mechanisms that take several days to become protective and are designed to remove a specific antigen. This is the immunity one develops throughout life. There are two major branches of the adaptive immune responses: 1. Humoral immunity ...
5 Conclusion - Duke Computer Science
5 Conclusion - Duke Computer Science

... We have made significant progress with experimental implementation of DNA-based computers which perform calculations during self-assembly of specific nanoscale tilings. We have successfully prototyped a novel read-in method for tiling-based computers: we have demonstrated for the first time that lon ...
CovarisPCRtube
CovarisPCRtube

... Clean your bench with bleach (84) every time after you did DNA extraction or fish dissection. Clean your pipettors, rack and bench space with bleach once a while. Aliquot all reagents into appropriate amount, so not to contaminate the whole tube of reagents. Make sure you have enough filtered tips, ...
T4 helper cell
T4 helper cell

...  Protects body from infection caused by bacteria, viruses, and other foreign invaders  Composed of chemicals and cells that attack and inactivate things that enter the body  First line of defense is the skin, blocks invaders  T cells and B cells: white blood cells, more specific forms of protect ...
BTCH Reg Course Rev Sem2
BTCH Reg Course Rev Sem2

... Plasmids are very important pieces of DNA. How do they differ from chromosomal DNA molecules? ...
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DNA vaccination



DNA vaccination is a technique for protecting an animal against disease by injecting it with genetically engineered DNA so cells directly produce an antigen, resulting in a protective immunological response. Several DNA vaccines have been released for veterinary use, and there has been promising research using the vaccines for viral, bacterial and parasitic diseases, as well as to several tumour types. Although only one DNA vaccine has been approved for human use, DNA vaccines may have a number of potential advantages over conventional vaccines, including the ability to induce a wider range of immune response types.
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