(BIOLOGY) CBSE-XII-2013 EXAMINATION BIOLOGY CAREER POINT
... In humans (and most vertebrates), the first polar body does not undergo meiosis II, whereas the secondary oocyte proceeds as far as the metaphase stage of meiosis II. However, it then stops advancing any further, it awaits the arrival of the spermatozoa for completion of second meiotic division. Ent ...
... In humans (and most vertebrates), the first polar body does not undergo meiosis II, whereas the secondary oocyte proceeds as far as the metaphase stage of meiosis II. However, it then stops advancing any further, it awaits the arrival of the spermatozoa for completion of second meiotic division. Ent ...
Polymerase Chain Reaction (PCR)
... 4- To remove soluble proteins and other materials, we mixing with chloroform and centrifugation. 5- DNA must be then be precipitated from the aqueous phase and washed thoroughly to remove contaminating salts. 6- To purified DNA, then re-suspended and store in TE buffer or sterile ...
... 4- To remove soluble proteins and other materials, we mixing with chloroform and centrifugation. 5- DNA must be then be precipitated from the aqueous phase and washed thoroughly to remove contaminating salts. 6- To purified DNA, then re-suspended and store in TE buffer or sterile ...
TUMOR IMMUNOLOGY
... • Both active and passive means of stimulating the non-specific and specific immune systems have been employed, in some cases with significant success ...
... • Both active and passive means of stimulating the non-specific and specific immune systems have been employed, in some cases with significant success ...
IMMUNOLOGIC METHODS шкільна
... Electroimmunodiffusion-use of an electric field to rapidly drive reactants toward each other: Counterimmunoelectrophoresis (one dimensional double electroimmunodiffusion) -antigen and antibody rapidly diffuse in gel medium toward each other, leading to semiquantitative measurement of antigen in biol ...
... Electroimmunodiffusion-use of an electric field to rapidly drive reactants toward each other: Counterimmunoelectrophoresis (one dimensional double electroimmunodiffusion) -antigen and antibody rapidly diffuse in gel medium toward each other, leading to semiquantitative measurement of antigen in biol ...
Final Exam Practice
... late one night you come upon an enclave of gnomes. You are struck by the color of their beards, which are all blue. (Gnomes are diploid organisms, both male and female gnomes have beards, and you can assume that the gnomes are true-breeding for this trait.) The following week you are busy pulling a ...
... late one night you come upon an enclave of gnomes. You are struck by the color of their beards, which are all blue. (Gnomes are diploid organisms, both male and female gnomes have beards, and you can assume that the gnomes are true-breeding for this trait.) The following week you are busy pulling a ...
Ch. 1 Plasmids
... antibiotic selection to select for those bacteria that took up the plasmid that contains an antibiotic resistance gene. It is worth noting that bacteria are not the only cells that can be “transformed” with foreign DNA. One can also transform yeast, insect, plant and mammalian cells with vectors con ...
... antibiotic selection to select for those bacteria that took up the plasmid that contains an antibiotic resistance gene. It is worth noting that bacteria are not the only cells that can be “transformed” with foreign DNA. One can also transform yeast, insect, plant and mammalian cells with vectors con ...
The immune system and new therapies for
... roles of macrophages is to work as APCs although they play numerous roles as part of the immune army. Macrophages are distributed throughout the body in tissues and blood and have the potential to consume passing antigens and immune complexes by cleaning up debris throughout the body’s immune system ...
... roles of macrophages is to work as APCs although they play numerous roles as part of the immune army. Macrophages are distributed throughout the body in tissues and blood and have the potential to consume passing antigens and immune complexes by cleaning up debris throughout the body’s immune system ...
Chapter06_Outline
... the amplification of a particular DNA fragment • Oligonucleotide primers that are complementary to the ends of the target sequence are used in repeated rounds of denaturation, annealing, and DNA replication • The number of copies of the target sequence doubles in each round of replication, eventuall ...
... the amplification of a particular DNA fragment • Oligonucleotide primers that are complementary to the ends of the target sequence are used in repeated rounds of denaturation, annealing, and DNA replication • The number of copies of the target sequence doubles in each round of replication, eventuall ...
A System of Many Hats
... 1. Person gets a cut; pathogens (e.g. bacteria) enter. 2. One macrophage recognizes the pathogen as foreign and eats it. (Put an “RIP” sign on this bacteria). The Macrophage takes the antigen from this bacteria to the lymph node to activate T and B cells. 3. In the meantime, the other bacteria is ab ...
... 1. Person gets a cut; pathogens (e.g. bacteria) enter. 2. One macrophage recognizes the pathogen as foreign and eats it. (Put an “RIP” sign on this bacteria). The Macrophage takes the antigen from this bacteria to the lymph node to activate T and B cells. 3. In the meantime, the other bacteria is ab ...
Whole blood assays for interferon-I`: practicalities and potential for
... Responses to a panel of purified protein derivatives (PPDs) from a range of mycobacterial species have been used in the Malawi study to assess exposure to pathogenic and environ mental mycobacteria. Using the antigens at a concentration of 5 p,g per mI, and a cut-off of 62 pg per mI of IFN-'Y to de ...
... Responses to a panel of purified protein derivatives (PPDs) from a range of mycobacterial species have been used in the Malawi study to assess exposure to pathogenic and environ mental mycobacteria. Using the antigens at a concentration of 5 p,g per mI, and a cut-off of 62 pg per mI of IFN-'Y to de ...
Automation of genomic DNA isolation from formalin
... from these samples remains a challenge for clinical laboratories and researchers screening multiple samples for genetic variants as conventional extraction procedures are very laborious and timeconsuming for processing of samples in a busy clinical laboratory setting. Nevertheless, FFPE tissue archi ...
... from these samples remains a challenge for clinical laboratories and researchers screening multiple samples for genetic variants as conventional extraction procedures are very laborious and timeconsuming for processing of samples in a busy clinical laboratory setting. Nevertheless, FFPE tissue archi ...
Lecture 17 Protein synthesis pp101-110
... small subunit of the ribosome • Methionine-charged initiator tRNA binds to the small subunit • The large ribosomal unit now binds to this complex forming a functional ribosome ...
... small subunit of the ribosome • Methionine-charged initiator tRNA binds to the small subunit • The large ribosomal unit now binds to this complex forming a functional ribosome ...
Work Day 2
... urination and pus release from the urethra Many women do not experience noticeable symptoms ...
... urination and pus release from the urethra Many women do not experience noticeable symptoms ...
You are a Body Cell!
... 1. Person gets a cut; pathogens (e.g. bacteria) enter. 2. One macrophage recognizes the pathogen as foreign and eats it. (Put an “RIP” sign on this bacteria). The Macrophage takes the antigen from this bacteria to the lymph node to activate T and B cells. 3. In the meantime, the other bacteria is ab ...
... 1. Person gets a cut; pathogens (e.g. bacteria) enter. 2. One macrophage recognizes the pathogen as foreign and eats it. (Put an “RIP” sign on this bacteria). The Macrophage takes the antigen from this bacteria to the lymph node to activate T and B cells. 3. In the meantime, the other bacteria is ab ...
Biochemical and genetic characterization of the
... The open reading frame, YOR005c, encodes the activity previously designated as S.cerevisiae DNA ligase II In a previous study we described the partial purification and characterization of a 90 kDa DNA ligase that we designated DNA ligase II, since it had different biochemical properties than Cdc9 DN ...
... The open reading frame, YOR005c, encodes the activity previously designated as S.cerevisiae DNA ligase II In a previous study we described the partial purification and characterization of a 90 kDa DNA ligase that we designated DNA ligase II, since it had different biochemical properties than Cdc9 DN ...
Non-directed Modification of Genome Cont.. - PMAS
... • Usual transfection methods are not efficient for mammal oocytes and embryos as they are exclusively for somatic cells • Carbon nanotubes have emerged as a new method for gene delivery, and they can be an alternative for embryos transfection ...
... • Usual transfection methods are not efficient for mammal oocytes and embryos as they are exclusively for somatic cells • Carbon nanotubes have emerged as a new method for gene delivery, and they can be an alternative for embryos transfection ...
Simple and straightforward construction of a mouse gene targeting
... a gene targeting experiment is laborious and time-consuming, demanding elaborate techniques for the manipulation of DNA, cells and embryos, and it often takes more than one year from the design of constructs to generation of animals. Replacement of the native gene with a modified gene fragment in th ...
... a gene targeting experiment is laborious and time-consuming, demanding elaborate techniques for the manipulation of DNA, cells and embryos, and it often takes more than one year from the design of constructs to generation of animals. Replacement of the native gene with a modified gene fragment in th ...
Chapter 19 Disorders Associated with the Immune System
... Acquired immunodeficiency syndrome (AIDS) is a type of immunodeficiency disease. It is caused by the human immunodeficiency virus (HIV), which destroys helper T cells. AIDS is the final stage of a lengthy HIV infection. At this time the loss of an effective immune system leaves the victim susceptibl ...
... Acquired immunodeficiency syndrome (AIDS) is a type of immunodeficiency disease. It is caused by the human immunodeficiency virus (HIV), which destroys helper T cells. AIDS is the final stage of a lengthy HIV infection. At this time the loss of an effective immune system leaves the victim susceptibl ...
Document
... The Genetic Code ■ A codon designates an amino acid ■ An amino acid may have more than one codon ■ There are 20 amino acids, but 64 possible codons ■ Some codons tell the ribosome to stop translating ...
... The Genetic Code ■ A codon designates an amino acid ■ An amino acid may have more than one codon ■ There are 20 amino acids, but 64 possible codons ■ Some codons tell the ribosome to stop translating ...
Protein Synthesis
... DNA triplets encode for each one of the 20 amino acids that make proteins • During transcription, a DNA triplet will produce an mRNA codon. • During translation, a codon will constitute an amino acid ...
... DNA triplets encode for each one of the 20 amino acids that make proteins • During transcription, a DNA triplet will produce an mRNA codon. • During translation, a codon will constitute an amino acid ...
A general and rapid mutagenesis method using polymerase chain
... (Methfessel et al., 1986). Methods for mutagenesis using PCR have been described employing oligo primers containing restriction sites (Kadowaki et al., 1989), or covering regions containing restriction sites useful for replacing the wt sequence (Vallette et al., 1989). In addition, the introduction ...
... (Methfessel et al., 1986). Methods for mutagenesis using PCR have been described employing oligo primers containing restriction sites (Kadowaki et al., 1989), or covering regions containing restriction sites useful for replacing the wt sequence (Vallette et al., 1989). In addition, the introduction ...
Numerical Evidence for Nucleated Self
... that the critical cluster size has been correctly identified, although the precise structure of a given cluster has a significant effect when considering its propensity to grow or shrink: the cluster size alone is not an optimal order parameter. At lower temperatures, where the nucleation free-energ ...
... that the critical cluster size has been correctly identified, although the precise structure of a given cluster has a significant effect when considering its propensity to grow or shrink: the cluster size alone is not an optimal order parameter. At lower temperatures, where the nucleation free-energ ...
DNA vaccination
DNA vaccination is a technique for protecting an animal against disease by injecting it with genetically engineered DNA so cells directly produce an antigen, resulting in a protective immunological response. Several DNA vaccines have been released for veterinary use, and there has been promising research using the vaccines for viral, bacterial and parasitic diseases, as well as to several tumour types. Although only one DNA vaccine has been approved for human use, DNA vaccines may have a number of potential advantages over conventional vaccines, including the ability to induce a wider range of immune response types.