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DNA - Fort Bend ISD
DNA - Fort Bend ISD

... template for a new strand of DNA 2. Steps of replication: a) Enzymes unzip DNA breaking hydrogen bonds between bases. ...
Rethinking Gene Expression and Evolution (Nobel Lecture)
Rethinking Gene Expression and Evolution (Nobel Lecture)

... perfect copy of the strand to which its bound simply by unwinding and allowing the polymerase to copy it. In Roger Kornbergs talk, we heard about an RNA polymerase that can transcribe the DNA to produce RNA copies of the genetic information. These copies provide templates for the polymerization of ...
Mapping Mutations in the HIV RNA
Mapping Mutations in the HIV RNA

... Virus Overview Viruses may be defined as acellular organisms whose genomes consist of nucleic acid, and which obligately replicate inside host cells using host metabolic machinery and ribosomes to form a pool of components which assemble into particles called VIRIONS, which serve to protect the gen ...
DNA - The Double Helix
DNA - The Double Helix

... molecules. The sugar is a pentose called deoxyribose. Color all the phosphates pink (one is labeled with a "p"). Color all the deoxyribose sugars blue (one is labeled with a "D"). What is meant by a double helix? ____________________________ Name a pentose sugar. _________________ The sides of DNA a ...
Editing of a tRNA anticodon in marsupial
Editing of a tRNA anticodon in marsupial

... mitochondrial tRNAIle has been shown to be posttranscriptionally converted from a cytidine to a lysidine-like residue which will base pair with adenosine rather than guanosine residues (15). It is highly unlikely that such a modification is involved in the marsupial tRNAAjP since reverse transcripta ...
Polypeptide Synthesis -Making Proteins
Polypeptide Synthesis -Making Proteins

... 5. The next tRNA with the appropriate amino acid enters the A site matching to the mRNA codon. - Determine the amino acid coming in based on the mRNA codon sequence – use mRNA codon chart. ...
Special Study Project III
Special Study Project III

... 42. It became apparent to Watson and Crick after completion of their model that DNA molecule could carry a vast amount of hereditary information in its a. sequence of bases b. phosphate-sugar backbone c. complementary base pairing d. side groups of nitrogenous bases e. different five-carbon sugars ...
RiboMAX(TM) Large Scale RNA Production Systems
RiboMAX(TM) Large Scale RNA Production Systems

... incorporation of radiolabeled ribonucleotides rather than to produce large amounts of RNA. However, in vitro transcription also is used for other applications that require larger amounts of biologically active RNA including in vitro translation and for synthesis of tRNA, rRNA, other small functional ...
Protein synthesis
Protein synthesis

... (rRNA). This form of RNA is used to manufacture ribosomes. Ribosomes are ultramicroscopic particles of rRNA and protein. They are the places (the chemical “workbenches”) where amino acids are linked to one another to synthesize proteins. Ribosomes may exist along the membrane of the endoplasmic reti ...
Structure of Nucleic Acids
Structure of Nucleic Acids

... Deoxyribonucleic acid ) (DNA) is a nucleic acid that contains the genetic instructions used in the development and functioning of all known living organismswith the exception of some viruses. The main role of DNA molecules is the long-term storage ofinformation. DNA is often compared to a set of blu ...
final review
final review

... B) DNA is a polymer composed of nucleotides, whereas RNA is a polymer composed of nucleic acids. C) DNA is double-stranded, whereas RNA is single-stranded. D) DNA contains five-carbon sugars, whereas RNA contains six-carbon sugars. E) DNA contains nitrogenous bases, whereas RNA contains phosphate gr ...
Molecular Genetics
Molecular Genetics

... A gene is a segment of DNA that specifies the sequence of amino acids in a polypeptide  Suggests that genetic mutations cause changes in the primary structure of a protein ...
Macromolecular Sequence Analysis Biological sequences
Macromolecular Sequence Analysis Biological sequences

... §  messenger RNA (mRNA): Their role is to mediate the synthesis of proteins from the DNA (genes). They are synthesized during the transcription and are used during the translation as a template to build proteins. §  transfer RNA (tRNA): Amino acids do not recognise RNA codon directly. The role of ...
DNA Replication
DNA Replication

... In the elongation stage the amino acids are added one by one to form a protein. This stage has three steps: a) Aminoacyl-tRNA binding. At the start of this step the fMet-tRNA complex is bound to the P (peptidyl) site of the ribosome. The other site of the ribosome – the A (aminoacyl) site – is empty ...
RNA polymerase
RNA polymerase

...  evolved to maintain constant internal conditions while facing changing external conditions ...
origins debate intro
origins debate intro

... does not have catalytic ability and so cannot replicate on its own. It needs proteins. Next, let’s assume that proteins arose first. Proteins are very versatile and can carry out a range of catalytic reactions. Unfortunately, they have no easy way of storing and passing on the information for making ...
LIPIDS
LIPIDS

... Transfer RNA = tRNA ...
Chance and Necessity in the Selection of Nucleic Acid Catalysts
Chance and Necessity in the Selection of Nucleic Acid Catalysts

... using the tag as a handle. The tag could be, for example, a biotin group, and the “tag grabber” could be an immobilized streptavidin resin. The active sequences are amplified as in Figure 1, and the cycle can then be repeated. By derivatizing the RNA or DNA with a second substrate molecule, catalyst ...
A Search for Genes Encoding Histidine
A Search for Genes Encoding Histidine

... We propose the following regulation mechanism. For brevity, we assume that the rate of leader peptide translation depends on histidine concentration. If it is deficient, the ribosome translating the leader peptide does not reach the stop codon and an RNA hairpin is formed to prevent initiation of st ...
High-Salt Precipitation Solution Manual ver.1
High-Salt Precipitation Solution Manual ver.1

... Solution Manual ver.1 Code No. 313-06341 ...
si RNA
si RNA

... The use of RNA interference for artificially manipulating gene expression was initially limited by the activation of cellular antiviral mechanisms. Exposure of cells to sequences longer than 30 nucleotides induces interferon gene expression resulting in non-specific RNA degradation and reduced prote ...
Protein Synthesis - Napa Valley College
Protein Synthesis - Napa Valley College

... 1. RNA is single stranded, DNA is double stranded 2. The sugar is different = ribose 3. RNA has four bases, but one base is different from DNA: CGAU, the U is uracil During transcription uracil is paired with adenine ...
Transcription and Translation
Transcription and Translation

... Each triplet code on a DNA molecule is transcribed into a triplet codon on the mRNA molecule. • If the DNA codes for a polypeptide is T-A-C—C-C-G—T-A-G—C-T-T—A-C-T • What would the codons on the complimentary strand of mRNA codons look like? A-U-G – G-G-C – A-U-C – G-A-A – U-G-A • DNA codes: T-A-C— ...
Homework Assignment #1
Homework Assignment #1

... by itself is very low and is invariably influenced by other specific transcription factors that bind either to the adjacent upstream promoter region (-120 to -30) or to more distant enhancer sites. Analysis of these regulatory regions indicates that they generally contain many binding sites for diff ...
Chapter 9 From DNA to Protein
Chapter 9 From DNA to Protein

... How is RNA Assembled? (cont’d.) • Transcription begins when an RNA polymerase and regulatory proteins attach to a DNA site called a promoter – RNA polymerase moves over a gene region and unwinds the double helix a bit so it can “read” the base sequence of the DNA strand – The polymerase joins free ...
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RNA



Ribonucleic acid (RNA) is a polymeric molecule implicated in various biological roles in coding, decoding, regulation, and expression of genes. RNA and DNA are nucleic acids, and, along with proteins and carbohydrates, constitute the three major macromolecules essential for all known forms of life. Like DNA, RNA is assembled as a chain of nucleotides, but unlike DNA it is more often found in nature as a single-strand folded onto itself, rather than a paired double-strand. Cellular organisms use messenger RNA (mRNA) to convey genetic information (using the letters G, U, A, and C to denote the nitrogenous bases guanine, uracil, adenine, and cytosine) that directs synthesis of specific proteins. Many viruses encode their genetic information using an RNA genome.Some RNA molecules play an active role within cells by catalyzing biological reactions, controlling gene expression, or sensing and communicating responses to cellular signals. One of these active processes is protein synthesis, a universal function whereby mRNA molecules direct the assembly of proteins on ribosomes. This process uses transfer RNA (tRNA) molecules to deliver amino acids to the ribosome, where ribosomal RNA (rRNA) links amino acids together to form proteins.
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