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General Replication Strategies for RNA Viruses
General Replication Strategies for RNA Viruses

... Small nuclear RNA (snRNA) is involved in pre-mRNA splicing. Heterogenous nuclear RNA (hnRNA) is the primary transcript from the eukaryotic enzyme, RNA polymerase II. hnRNA is the precursor of all mRNA often called "premRNA", prior to the removal of introns. Small nucleolar RNA (snoRNA) is found in t ...
CELL CHEMISTRY QUESTIONS 1. - Queensland Science Teachers
CELL CHEMISTRY QUESTIONS 1. - Queensland Science Teachers

... 22. Different types of cells have different proteins. How can this be used to identify cell types? 23. Is every protein composed of all possible amino acids? Explain. 24. Why are some amino acids called essential amino acids? 25. What are some of the many functions of proteins? 26. Nucleic acids are ...
Name
Name

... 7. Transcription in prokaryotes and eukaryotes is similar in that: a. transcriptional machinery controls compaction and decompaction of chromatin b. the mRNA produced can undergo alternative splicing c. both prokaryotic and eukaryotic proteins have identical affinities for DNA d. both are contained ...
Nucleic acid
Nucleic acid

... genetic instructions used in the development and functioning of all known living organisms. The DNA segments carrying this genetic information are called genes Likewise, other DNA sequences have structural purposes, or are involved in regulating the use of this genetic information. Along with RNA an ...
Exam 3
Exam 3

... Know the anatomy and function of a “T–even” bacteriophage. Know the lytic cycle, so to be able to match the five major steps with a description of each. Again, know rolling circle replication of viral genomes. Understand that a bacterial cell with a virus engaged in the lytic cycle will soon die. Wh ...
THE DEVELOPMENT OF AN RNA BASED ASSAY SYSTEM TO
THE DEVELOPMENT OF AN RNA BASED ASSAY SYSTEM TO

... The present work describes a prototype RNA based assay system to supplant conventional methods for body fluid identification. Before discussing the assay in detail, it is important to consider whether there is still a need for such testing. Prior to the advent of DNA analysis, it was standard practi ...
Introduction to molecular biology
Introduction to molecular biology

... • Other RNA fucntions related with trasncription regulation – miRNA: small pieces of RNA that interrupt the transcription of a gene ...
Name
Name

... a-Groups of _____ nitrogen bases are translated into amino acids. These trios bases are called _____________________, and they are located on ___RNA molecules. Amino acids are the building blocks of ________________________. ...
Protein Synthesis: Transcription
Protein Synthesis: Transcription

... tRNA: transports amino acids to the mRNA to make the protein rRNA: make up the ribosomes, which help make the protein ...
Class 27
Class 27

... Aptamers: molecules that bind other molecules with good affinity and specificity Usually these are proteins . . . . But they can also be RNA or DNA. That is, single stranded RNA or DNA molecules can and will fold up into secondary and tertiary structures depending on their sequence. DNA can be synth ...
Gene Expression and Gene Regulation
Gene Expression and Gene Regulation

... the covalent bonding of many nucleotides to one another to form an RNA strand. The base sequence of the new RNA strand is complementary to the base sequence of its DNA template––a copy of the gene. ...
You Light Up My Life
You Light Up My Life

... of the RNA polymerase. Short lengths of the newly forming RNA strand briefly wind up with its DNA template strand. New stretches of RNA unwind from the template (and the two DNA strands wind up again). ...
1. A 6-frame translation map of a segment of DNA is shown, with
1. A 6-frame translation map of a segment of DNA is shown, with

... 2b. Complete your corrected diagram to show RNA transcripts of relatively correct length on the RNA polymerases. Transcription goes right-to-left through ORF B (because the Crick strand is the coding strand) so, the RNA transcripts will be smallest at the right (where transcription has just begun) a ...
Bio1001Ch13W
Bio1001Ch13W

... Summary- The genetic code uses_ ____________________, or codons, each of which is translated into a specific amino acid. ...
Transcription, Transcription and Mutations
Transcription, Transcription and Mutations

...  To compare and contrast DNA and RNA  To identify the steps of transcription  To define the enzymes involved ...
File
File

... – RNA polymerase and other proteins form a transcription complex. – The transcription complex recognizes the start of a gene and unwinds a segment of it. start site ...
Protein Synthesis
Protein Synthesis

... Definition - A linear polymer of amino acids linked together in a specific order or sequence. Formed in the process known as translation. ...
experimental design
experimental design

... generation of first strand cDNA in a 25µl reaction volume. 1µg of RNA (5~10µl), 1µl of oligo(dT)18 (500µg/ml) and 1µl dNTP mix (Invitrogen, Catalog No. 18427) were incubated at 70℃ for 10 min and quick chilled on ice. All other steps were performed according to manufacturer’s instructions except tha ...
Assignment 5 (Perl Project 2)
Assignment 5 (Perl Project 2)

... and t in any order. For example, acgtacccggttt is a small DNA strand. The four letters stand for the four nucleotides : adenine, cytosine, guanine, and thymine. Nucleotides, which are the molecular units from which DNA and RNA are composed, are also called bases. A special enzyme called RNA polymera ...
DNA, and in some cases RNA, is the primary source of heritable
DNA, and in some cases RNA, is the primary source of heritable

... parts of an RNA transcript, different mRNA's can be produced, each coding for a different protein product. Thus the number of different proteins an organism can produce is much greater than its number of genes. Check out the mRNA processing activity in your online textbook. It will help you understa ...
Introduction and Review
Introduction and Review

... sequences. An initiation codon (AUG) is oriented on the small ribosome subunit. AUG is the codon for the amino acid methionine. Please note: Sequences on mRNA are listed, by convention, in the 5’  3’ direction ...
Cracking the PPR code: predicting and manipulating protein/RNA
Cracking the PPR code: predicting and manipulating protein/RNA

... Mentor: Margarita Rojas PI: Alice Barkan ...
Chapter 10: How Proteins are Made
Chapter 10: How Proteins are Made

... – Contains ribose as sugar – Contains uracil (U) as complementary base pair to adenine (A) ...
ppt presentation
ppt presentation

... The precise role of 25-nt RNA in PTGS remains to be determined. However, because they are long enough to convey sequence specificity yet small enough to move through plasmodesmata, it is possible that they are ...
Practical molecular biology
Practical molecular biology

... Separating DNA from other cellular components such as proteins, lipids, RNA, etc. Avoiding fragmentation of the long DNA molecules by mechanical shearing or the action of endogenous nucleases. Effectively inactivating endogenous nucleases (DNase enzymes) and preventing them from digesting the genomi ...
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RNA



Ribonucleic acid (RNA) is a polymeric molecule implicated in various biological roles in coding, decoding, regulation, and expression of genes. RNA and DNA are nucleic acids, and, along with proteins and carbohydrates, constitute the three major macromolecules essential for all known forms of life. Like DNA, RNA is assembled as a chain of nucleotides, but unlike DNA it is more often found in nature as a single-strand folded onto itself, rather than a paired double-strand. Cellular organisms use messenger RNA (mRNA) to convey genetic information (using the letters G, U, A, and C to denote the nitrogenous bases guanine, uracil, adenine, and cytosine) that directs synthesis of specific proteins. Many viruses encode their genetic information using an RNA genome.Some RNA molecules play an active role within cells by catalyzing biological reactions, controlling gene expression, or sensing and communicating responses to cellular signals. One of these active processes is protein synthesis, a universal function whereby mRNA molecules direct the assembly of proteins on ribosomes. This process uses transfer RNA (tRNA) molecules to deliver amino acids to the ribosome, where ribosomal RNA (rRNA) links amino acids together to form proteins.
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