RecQ-like helicases and the DNA replication checkpoint
... controlled by two SCB (Swi4p-Swi6p cell cycle box) elements that are typically found in the promoters of genes whose products are required for late G1 or S phase progression. More striking, however, is the sharp fluctuation in the levels of Sgs1 protein: Sgs1p is abundant in S phase, but is barely d ...
... controlled by two SCB (Swi4p-Swi6p cell cycle box) elements that are typically found in the promoters of genes whose products are required for late G1 or S phase progression. More striking, however, is the sharp fluctuation in the levels of Sgs1 protein: Sgs1p is abundant in S phase, but is barely d ...
The relationship between higher‑order chromatin structure and
... have reasonable models for. However, it is clear from electron microscopy studies that a lot of mammalian chromatin is packaged into levels beyond this. The few assays that we have for tertiary levels of chromatin structure are, in the main, cytological. There does seem to be a link between chromati ...
... have reasonable models for. However, it is clear from electron microscopy studies that a lot of mammalian chromatin is packaged into levels beyond this. The few assays that we have for tertiary levels of chromatin structure are, in the main, cytological. There does seem to be a link between chromati ...
Python Practice
... one line at a time as the program is running b. A program that reads and executes source code one line at a time. Does not create an executable file that can run independently. c. A program that reads, interprets, and executes a program, eliminating the need for compiling source code. Running a prog ...
... one line at a time as the program is running b. A program that reads and executes source code one line at a time. Does not create an executable file that can run independently. c. A program that reads, interprets, and executes a program, eliminating the need for compiling source code. Running a prog ...
1. The cross AaBb x AaBb is called a
... c. 25; d. 37.5; e. 50. 37. In maize, two plants that are heterozygous for the recessive alleles a and b are crossed, what frequency of double-mutant progeny will appear if a and b are 7.2 map units apart, and both parents carry a and b in trans (configuration)? a. 0.036; b. 0.0625; c. 0.001296 d. 0. ...
... c. 25; d. 37.5; e. 50. 37. In maize, two plants that are heterozygous for the recessive alleles a and b are crossed, what frequency of double-mutant progeny will appear if a and b are 7.2 map units apart, and both parents carry a and b in trans (configuration)? a. 0.036; b. 0.0625; c. 0.001296 d. 0. ...
Structure and function of nucleases in DNA repair: shape
... and the numbers of metals, are very complicated, but are not common among the nucleases. It appears that the major role of the metals is to stabilize intermediates, thereby facilitating the phosphoryl transfer reactions. Cleavage reactions occur either at the end or within DNA, and thus DNA nuclease ...
... and the numbers of metals, are very complicated, but are not common among the nucleases. It appears that the major role of the metals is to stabilize intermediates, thereby facilitating the phosphoryl transfer reactions. Cleavage reactions occur either at the end or within DNA, and thus DNA nuclease ...
Analyzing the Changes in DNA Flexibility Due to Base Modifications
... Now, we have the structure of a dsDNA helix in the file nab.pdb. Using this PDB file, we can create the input files for NAMD simulations (PSF and PDB files) using MDfiles/psfgen.tcl. We must first split the nab.pdb file into nab1.pdb and nab2.pdb, each of which contain one strand (this is due to the ...
... Now, we have the structure of a dsDNA helix in the file nab.pdb. Using this PDB file, we can create the input files for NAMD simulations (PSF and PDB files) using MDfiles/psfgen.tcl. We must first split the nab.pdb file into nab1.pdb and nab2.pdb, each of which contain one strand (this is due to the ...
Aimhigher Monitoring Template
... in length, 16.9% thought it too short and only 8.8% thought it too long. This data was similar to that collected in 2006. The teachers felt that they had all received adequate information about the event before it took place and gave the organisation a rating of 4.5. We did arrive with little time t ...
... in length, 16.9% thought it too short and only 8.8% thought it too long. This data was similar to that collected in 2006. The teachers felt that they had all received adequate information about the event before it took place and gave the organisation a rating of 4.5. We did arrive with little time t ...
Eukaryotic RNA Polymerases and their Promoters
... – Upstream promoter element (UPE) 100 bp farther upstream – Spacing between these elements is important ...
... – Upstream promoter element (UPE) 100 bp farther upstream – Spacing between these elements is important ...
Detection of Antioxidative Activity of Plant Extracts at the DNA-modified Screen-printed Electrode
... substrate markedly delay or prevent the oxidation of the substrate [1]. They are used in the food industry to delay the oxidation process [2, 3]. The most active dietary antioxidants belong to the family of phenolic and polyphenolic compounds. Phenolic acids are widely distributed in the plant kingd ...
... substrate markedly delay or prevent the oxidation of the substrate [1]. They are used in the food industry to delay the oxidation process [2, 3]. The most active dietary antioxidants belong to the family of phenolic and polyphenolic compounds. Phenolic acids are widely distributed in the plant kingd ...
Lecture Presentation to accompany Principles of Life
... Concept 9.2 DNA Replicates Semiconservatively ...
... Concept 9.2 DNA Replicates Semiconservatively ...
Improvement of DNA Extraction Protocols for Nostochopsis spp.
... Nostochopsis spp. are a type of cyanobacteria which form mucilaginous balls. These cyanobacteria have a high content of polysaccharides, which makes it difficult to isolate their genomic DNA by the conventional method. In this research study, six protocols for improvement of DNA extraction from this ...
... Nostochopsis spp. are a type of cyanobacteria which form mucilaginous balls. These cyanobacteria have a high content of polysaccharides, which makes it difficult to isolate their genomic DNA by the conventional method. In this research study, six protocols for improvement of DNA extraction from this ...
KOD -Plus
... includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim (such as the patented 5’ Nuclease Process claims in US Patents Nos. 5,210,015 and 5,487,972 ...
... includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim (such as the patented 5’ Nuclease Process claims in US Patents Nos. 5,210,015 and 5,487,972 ...
Structure analysis of FAAP24 reveals single-stranded DNA
... 1Z00:A) and hXPF (PDB: 1Z00:B) (Figure 1D). The RMSD values of these structural superimpositions are 1.5, 2.5 and 2.6 Å, respectively. It showed that helices α7α10 were generally well-superimposed between FAAP24 and ERCC1/XFP, while the helix α6 of FAAP24 and ERCC1/XPF showed a deflection angle of a ...
... 1Z00:A) and hXPF (PDB: 1Z00:B) (Figure 1D). The RMSD values of these structural superimpositions are 1.5, 2.5 and 2.6 Å, respectively. It showed that helices α7α10 were generally well-superimposed between FAAP24 and ERCC1/XFP, while the helix α6 of FAAP24 and ERCC1/XPF showed a deflection angle of a ...
How Relevant is the Escherichia coli UvrABC Model for Excision
... Several interesting implications emerge from these data. (1) The amino acid sequences of the ERCC-2, -3 and -6 gene products suggest that at least three DNA helicases are involved in mammalian excision repair (Weber et al. 1990; Weeda et al. 1990; C. Troelstra, unpublished results). The infered heli ...
... Several interesting implications emerge from these data. (1) The amino acid sequences of the ERCC-2, -3 and -6 gene products suggest that at least three DNA helicases are involved in mammalian excision repair (Weber et al. 1990; Weeda et al. 1990; C. Troelstra, unpublished results). The infered heli ...
Chapter 13
... The backbones of the two DNA strands are closer together on one side of the double helix (forming the minor groove) than on the other (forming the major groove). There are four possible configurations of the base pairs in the grooves. ...
... The backbones of the two DNA strands are closer together on one side of the double helix (forming the minor groove) than on the other (forming the major groove). There are four possible configurations of the base pairs in the grooves. ...
Laboratory 9: Plasmid Isolation
... 6. Add 350 μL of Buffer N3 and mix by inverting several times. This is a potassium acetate buffer that will cause precipitation of cell debris including chromosomal DNA. You should see the solution immediately turn cloudy. This may sit on ice until you are ready to centrifuge (next step). C. Purify ...
... 6. Add 350 μL of Buffer N3 and mix by inverting several times. This is a potassium acetate buffer that will cause precipitation of cell debris including chromosomal DNA. You should see the solution immediately turn cloudy. This may sit on ice until you are ready to centrifuge (next step). C. Purify ...
Intrastrand Self-complementary Sequences in Bacillus subtilis DNA
... 1973). This DNA reassociates with unimolecular kinetics, typical of intrastrand structures. Only the slowest eluting molecules of H strand DNA show an increased transforming activity following self-annealing (Fig. 1a). Hydroxyapatite chromatography, which can separate double- and single-stranded DNA ...
... 1973). This DNA reassociates with unimolecular kinetics, typical of intrastrand structures. Only the slowest eluting molecules of H strand DNA show an increased transforming activity following self-annealing (Fig. 1a). Hydroxyapatite chromatography, which can separate double- and single-stranded DNA ...
Objective 2.1 Lesson D Recombinant Organisms
... 2. As one member is recording the sequences, the other group member should be looking for these sequences within that cut your PLASMID DNA ONE TIME! Read below before you start looking through all of those letters. 3. Your job as a biochemist is to find a restriction enzyme that will Cut open your ...
... 2. As one member is recording the sequences, the other group member should be looking for these sequences within that cut your PLASMID DNA ONE TIME! Read below before you start looking through all of those letters. 3. Your job as a biochemist is to find a restriction enzyme that will Cut open your ...
Nucleosome
A nucleosome is a basic unit of DNA packaging in eukaryotes, consisting of a segment of DNA wound in sequence around eight histone protein cores. This structure is often compared to thread wrapped around a spool.Nucleosomes form the fundamental repeating units of eukaryotic chromatin, which is used to pack the large eukaryotic genomes into the nucleus while still ensuring appropriate access to it (in mammalian cells approximately 2 m of linear DNA have to be packed into a nucleus of roughly 10 µm diameter). Nucleosomes are folded through a series of successively higher order structures to eventually form a chromosome; this both compacts DNA and creates an added layer of regulatory control, which ensures correct gene expression. Nucleosomes are thought to carry epigenetically inherited information in the form of covalent modifications of their core histones.Nucleosomes were observed as particles in the electron microscope by Don and Ada Olins and their existence and structure (as histone octamers surrounded by approximately 200 base pairs of DNA) were proposed by Roger Kornberg. The role of the nucleosome as a general gene repressor was demonstrated by Lorch et al. in vitro and by Han and Grunstein in vivo.The nucleosome core particle consists of approximately 147 base pairs of DNA wrapped in 1.67 left-handed superhelical turns around a histone octamer consisting of 2 copies each of the core histones H2A, H2B, H3, and H4. Core particles are connected by stretches of ""linker DNA"", which can be up to about 80 bp long. Technically, a nucleosome is defined as the core particle plus one of these linker regions; however the word is often synonymous with the core particle. Genome-wide nucleosome positioning maps are now available for many model organisms including mouse liver and brain.Linker histones such as H1 and its isoforms are involved in chromatin compaction and sit at the base of the nucleosome near the DNA entry and exit binding to the linker region of the DNA. Non-condensed nucleosomes without the linker histone resemble ""beads on a string of DNA"" under an electron microscope.In contrast to most eukaryotic cells, mature sperm cells largely use protamines to package their genomic DNA, most likely to achieve an even higher packaging ratio. Histone equivalents and a simplified chromatin structure have also been found in Archea, suggesting that eukaryotes are not the only organisms that use nucleosomes.