University of Groningen Characterization of the lytic-lysogenic
... region. A helix-swap of the a3 helices of the closely related CI-type proteins from the lactococcal phages r1t and Tuc2009 revealed the crucial elements involved in DNA recognition while also pointing to conserved functional properties of phage CI proteins infecting different hosts. CItype proteins ...
... region. A helix-swap of the a3 helices of the closely related CI-type proteins from the lactococcal phages r1t and Tuc2009 revealed the crucial elements involved in DNA recognition while also pointing to conserved functional properties of phage CI proteins infecting different hosts. CItype proteins ...
Human-Genetics-Concepts-and-Applications-9E
... direct-to-consumer genetic test panel and discovers that he has inherited gene variants that are associated with increased risk of alcoholism. He reports these facts during an interview for purchasing a life insurance policy, because he thinks that the Genetic Information Nondiscrimination Act will ...
... direct-to-consumer genetic test panel and discovers that he has inherited gene variants that are associated with increased risk of alcoholism. He reports these facts during an interview for purchasing a life insurance policy, because he thinks that the Genetic Information Nondiscrimination Act will ...
A group of interacting yeast DNA replication genes.
... Because CDC46 was initially isolated as an extragenic suppressor of two cold-sensitive mutations, cdc45 and cdc54, we investigated its genetic interactions with the other members of this group. The results of this analysis are summarized in Figure 1, which shows instances of both synthetic lethality ...
... Because CDC46 was initially isolated as an extragenic suppressor of two cold-sensitive mutations, cdc45 and cdc54, we investigated its genetic interactions with the other members of this group. The results of this analysis are summarized in Figure 1, which shows instances of both synthetic lethality ...
EPICENTRE Revolutionizes Cloning by Introducing CopyControl
... Select and grow CopyControl clones at single copy to better ensure insert stability and cloning of encoded, expressed toxic proteins. • Get high yields of DNA for sequencing, fingerprinting or other applications. CopyControl clones, in small culture volumes, can be readily amplified from single copy ...
... Select and grow CopyControl clones at single copy to better ensure insert stability and cloning of encoded, expressed toxic proteins. • Get high yields of DNA for sequencing, fingerprinting or other applications. CopyControl clones, in small culture volumes, can be readily amplified from single copy ...
The Recombinant DNA Controversy: A Contemporary
... restriction enzyme, recombined and ligated with DNA from another source (be it elephant or butterfly), and returned to the bacterium, the bacterium and its progeny will copy and recopy the recombinant DNA molecule millions of times, much like the copying machine I used in my analogy. The copying is ...
... restriction enzyme, recombined and ligated with DNA from another source (be it elephant or butterfly), and returned to the bacterium, the bacterium and its progeny will copy and recopy the recombinant DNA molecule millions of times, much like the copying machine I used in my analogy. The copying is ...
Transgenic and gene disruption techniques from a concept to a tool
... method is called the gene disruption tùh.tiqo".2'g If a DNA molecule carrying mutated mouse gene is transfered into a mouse cell, it usually inserted into the chromosomes at random sites and these nearly always ...
... method is called the gene disruption tùh.tiqo".2'g If a DNA molecule carrying mutated mouse gene is transfered into a mouse cell, it usually inserted into the chromosomes at random sites and these nearly always ...
Document
... A common fungicide (vinclozolin) used on grape plants causes low sperm count, prostate, and kidney disease in laboratory rats. ...
... A common fungicide (vinclozolin) used on grape plants causes low sperm count, prostate, and kidney disease in laboratory rats. ...
Screening of Gene Markers for Forensic Identification of Vaginal
... ,SERPIN B4 and SLPI .Eight genes out of these thirteen genes show expression the vaginal secretions. These genes are HBD1, MUC4, ADAMT S5, SLPI, CART1, KRT7, SPRR2B and HOXA13 (I). Other five genes namely SERPIN B4, INDO, CCL 20, CNFN and DEFA 5 show no expression at all. MUC4 and HBD1 were the gene ...
... ,SERPIN B4 and SLPI .Eight genes out of these thirteen genes show expression the vaginal secretions. These genes are HBD1, MUC4, ADAMT S5, SLPI, CART1, KRT7, SPRR2B and HOXA13 (I). Other five genes namely SERPIN B4, INDO, CCL 20, CNFN and DEFA 5 show no expression at all. MUC4 and HBD1 were the gene ...
Part 1 Microarray Timeseries Analysis with
... A reliable and precise classification of tumors is essential for successful diagnosis and treatment of cancer. Current methods for classifying human malignancies rely on a variety of morphological, clinical, and molecular variables. In spite of recent progress, there are still uncertainties in diagn ...
... A reliable and precise classification of tumors is essential for successful diagnosis and treatment of cancer. Current methods for classifying human malignancies rely on a variety of morphological, clinical, and molecular variables. In spite of recent progress, there are still uncertainties in diagn ...
Adobe PDF - Boston University Physics
... of simple repeats is that they constitute a large fraction of noncoding DNA, but are relatively rare in protein coding sequences [3]. Another reason for the interest in simple sequence repeats is their possible relation to the long-range correlations found in DNA sequences: recent studies [4,5] supp ...
... of simple repeats is that they constitute a large fraction of noncoding DNA, but are relatively rare in protein coding sequences [3]. Another reason for the interest in simple sequence repeats is their possible relation to the long-range correlations found in DNA sequences: recent studies [4,5] supp ...
Identification of a Novel Streptococcal Gene
... vive DNA damage by synthesizing through DNA lesions that block replication forks (63). In E. coli, almost all SOS-targeted UV mutagenesis results from the activity of PolV (53, 64), and the umuDC operon is the only SOS locus that must be induced for SOS mutagenesis (61). PolV consists of one molecul ...
... vive DNA damage by synthesizing through DNA lesions that block replication forks (63). In E. coli, almost all SOS-targeted UV mutagenesis results from the activity of PolV (53, 64), and the umuDC operon is the only SOS locus that must be induced for SOS mutagenesis (61). PolV consists of one molecul ...
Construction and genetic characterization of temperature-sensitive mutant alleles of the yeast actin gene.
... To generate a pool of mutagenized plasmids with all mutational changes fixed in a homoduplex form, the bisulfitetreated DNA was transformed into E. coli strain BD1528, which is deficient in the repair enzyme uracil-N-glycosylase (ung-). Plasmid DNA (pool 1) was prepared from a mixture of 4000 transf ...
... To generate a pool of mutagenized plasmids with all mutational changes fixed in a homoduplex form, the bisulfitetreated DNA was transformed into E. coli strain BD1528, which is deficient in the repair enzyme uracil-N-glycosylase (ung-). Plasmid DNA (pool 1) was prepared from a mixture of 4000 transf ...
Gene7-28
... suppresses growth or triggers apoptosis Figure 28.25 Damage to DNA activates p53. The outcome depends on the stage of the cell cycle. Early in the cycle, p53 activates a checkpoint that prevents further progress until the damage has been repaired. If it is too late to exercise the checkpoint, p53 tr ...
... suppresses growth or triggers apoptosis Figure 28.25 Damage to DNA activates p53. The outcome depends on the stage of the cell cycle. Early in the cycle, p53 activates a checkpoint that prevents further progress until the damage has been repaired. If it is too late to exercise the checkpoint, p53 tr ...
Consalez, GG, Stayton, CL, Freimer, NB, Goonewardena, Brown, WT, Gilliam, TC and Warren, ST: Isolation and characterization of a highly polymorphic human locus (DXS 455) in proximal Xq28. Genomics 12:710-714 (1992).
... with each of several enzymes. The map location of this cosmid insert was confirmed to be Xq28 by somatic cell hybrid analysis, and the polymorphism detected by ~346 (DXS 455) was found, as expected, to segregate in an X-linked fashion in CEPH reference pedigrees. Two unique subclones of ~346 were is ...
... with each of several enzymes. The map location of this cosmid insert was confirmed to be Xq28 by somatic cell hybrid analysis, and the polymorphism detected by ~346 (DXS 455) was found, as expected, to segregate in an X-linked fashion in CEPH reference pedigrees. Two unique subclones of ~346 were is ...
Decreased Expression of the p16/MTS1 Gene without
... no histological differences apparent between those cases with normal and those with decreased p16 expression. These results indicate that while p16 gene mutations may be rare, changes in the level of the p16 transcripts could play a role in human bladder carcinoma development. Key words: p16 – point ...
... no histological differences apparent between those cases with normal and those with decreased p16 expression. These results indicate that while p16 gene mutations may be rare, changes in the level of the p16 transcripts could play a role in human bladder carcinoma development. Key words: p16 – point ...
S-Phase Checkpoint Genes Safeguard High
... yeast (Lisby et al., 2001; Melo and Toczyski, 2002). Interestingly, foci containing some of the same repair proteins are detectable during S phase in human cells that have not been subjected to DNA-damaging agents (Costanzo et al., 2001; Maser et al., 2001; Mirzoeva and Petrini, 2003), suggesting th ...
... yeast (Lisby et al., 2001; Melo and Toczyski, 2002). Interestingly, foci containing some of the same repair proteins are detectable during S phase in human cells that have not been subjected to DNA-damaging agents (Costanzo et al., 2001; Maser et al., 2001; Mirzoeva and Petrini, 2003), suggesting th ...
De novo DNA cytosine methyltransferase activities in
... that mammalian DNA methyltransferases purified from somatic cells prefer hemimethylated DNA as substrate as would be expected for a maintenance methyltransferase (Gruenbaum et al., 1982; Bestor and Ingram, 1983; Pfeifer et al., 1983; reviewed by Adams et al., 1993). This property of the mammalian DN ...
... that mammalian DNA methyltransferases purified from somatic cells prefer hemimethylated DNA as substrate as would be expected for a maintenance methyltransferase (Gruenbaum et al., 1982; Bestor and Ingram, 1983; Pfeifer et al., 1983; reviewed by Adams et al., 1993). This property of the mammalian DN ...
385 Genetic Transformation : a Retrospective Appreciation
... (lower longer line) and donor chromosomal fragment (DNA molecule : upper shorter line), which could yield the observed transformants. The chromosomal regions marked -4are concerned with that part of the pathway of polysaccharide synthesis common to types I and I1 capsule ;those marked B determine th ...
... (lower longer line) and donor chromosomal fragment (DNA molecule : upper shorter line), which could yield the observed transformants. The chromosomal regions marked -4are concerned with that part of the pathway of polysaccharide synthesis common to types I and I1 capsule ;those marked B determine th ...
Chapter 13
... crystallographs from DNA samples. Her images suggested a doublestranded helix with 10 nucleotides in each full turn. The diameter of 2 nm suggested that the sugar-phosphate backbone of each strand must be on the outside. ...
... crystallographs from DNA samples. Her images suggested a doublestranded helix with 10 nucleotides in each full turn. The diameter of 2 nm suggested that the sugar-phosphate backbone of each strand must be on the outside. ...
General Pathology
... cells resemble the corresponding normal parenchymal cells,both morphologically and functionally, lack of differentiation is called anaplasia. In general benign tumors are well differentiated, malignant tumors are characterized by a wide range of parenchymal differentiation.,from Surprisingly well di ...
... cells resemble the corresponding normal parenchymal cells,both morphologically and functionally, lack of differentiation is called anaplasia. In general benign tumors are well differentiated, malignant tumors are characterized by a wide range of parenchymal differentiation.,from Surprisingly well di ...
Rethinking Gene Expression and Evolution (Nobel Lecture)
... understanding and manipulating the cellular basis of human disease, has had two extraordinary impacts on biological science. One is as a research tool: RNAi is now the state-of-the-art method by which scientists can knock down the expression of specific genes in cells, to thus define the biological ...
... understanding and manipulating the cellular basis of human disease, has had two extraordinary impacts on biological science. One is as a research tool: RNAi is now the state-of-the-art method by which scientists can knock down the expression of specific genes in cells, to thus define the biological ...
Exam 1
... 6. Describe a specific use for 3 of the following 5 types of mutations: missense mutations, nonsense mutations, frameshift mutations, deletion mutations, or insertion mutations. [6 points] There are many possible answers to this question, but the answer should describe a specific function for that p ...
... 6. Describe a specific use for 3 of the following 5 types of mutations: missense mutations, nonsense mutations, frameshift mutations, deletion mutations, or insertion mutations. [6 points] There are many possible answers to this question, but the answer should describe a specific function for that p ...
Notions of Biochemistry and Molecular Biology Manipulating DNA
... Attach the complementary strands (probes) to a filter and pour the solution through the filter Attach probes to tiny glass beads, place them in a glass column and pour the solution over them Attach probes to tiny magnetic beads, throw them in the solution and stir; attract them to one side using a m ...
... Attach the complementary strands (probes) to a filter and pour the solution through the filter Attach probes to tiny glass beads, place them in a glass column and pour the solution over them Attach probes to tiny magnetic beads, throw them in the solution and stir; attract them to one side using a m ...
Cancer epigenetics
Cancer epigenetics is the study of epigenetic modifications to the genome of cancer cells that do not involve a change in the nucleotide sequence. Epigenetic alterations are as important as genetic mutations in a cell’s transformation to cancer, and their manipulation holds great promise for cancer prevention, detection, and therapy. In different types of cancer, a variety of epigenetic mechanisms can be perturbed, such as silencing of tumor suppressor genes and activation of oncogenes by altered CpG island methylation patterns, histone modifications, and dysregulation of DNA binding proteins. Several medications which have epigenetic impact are now used in several of these diseases.