Effects of Salt Concentrations and Bending Energy on the Extent of
... controlling the properties of DNA in solution. Under many conditions of biological significance DNA is highly compact, and this can occur only when the repulsions between the phosphate groups are largely compensated by counterions and/or screened by added salt (1). Furthermore, it is known that inte ...
... controlling the properties of DNA in solution. Under many conditions of biological significance DNA is highly compact, and this can occur only when the repulsions between the phosphate groups are largely compensated by counterions and/or screened by added salt (1). Furthermore, it is known that inte ...
Imprinted gene detection in Arabidopsis thaliana
... Genetic imprinting is a form of epigenetic gene regulation that makes use of heritable molecular markers (imprints) which are attached to, but not part of the genome. They convey information about the parental origin of alleles to the offspring, and, depending on the gene, restrict its expression (t ...
... Genetic imprinting is a form of epigenetic gene regulation that makes use of heritable molecular markers (imprints) which are attached to, but not part of the genome. They convey information about the parental origin of alleles to the offspring, and, depending on the gene, restrict its expression (t ...
localization of histone gene transcripts in newt lampbrush
... great sequence purity. Such probes, after denaturation, are ideally suited for hybridizing to RNA transcripts on lampbrush chromosomes, and in this paper we describe what happens when echinoderm histone DNA sequences are applied in this manner. Histone coding sequences were chosen because the polype ...
... great sequence purity. Such probes, after denaturation, are ideally suited for hybridizing to RNA transcripts on lampbrush chromosomes, and in this paper we describe what happens when echinoderm histone DNA sequences are applied in this manner. Histone coding sequences were chosen because the polype ...
Correlations Between Gene Expression and Gene Conservation in
... levels, whereas a disproportionate percentage of the poorly expressed genes are organism specific; the expression levels of the latter increase during more specialized processes such as cellular Orphans Are Highly Enriched Among Genes Induced differentiation. Moreover, organism-specific genes are ov ...
... levels, whereas a disproportionate percentage of the poorly expressed genes are organism specific; the expression levels of the latter increase during more specialized processes such as cellular Orphans Are Highly Enriched Among Genes Induced differentiation. Moreover, organism-specific genes are ov ...
The past, present and future of plant breeding
... maize stay firmly attached to the cob. The same goes for other grain crops such as wheat, barley and rice. Besides developing new varieties (an example being the diversity of cruciferous vegetables) and useful traits (such as kernels that stay attached to the cob), humans have also tried to increase ...
... maize stay firmly attached to the cob. The same goes for other grain crops such as wheat, barley and rice. Besides developing new varieties (an example being the diversity of cruciferous vegetables) and useful traits (such as kernels that stay attached to the cob), humans have also tried to increase ...
ppt檔案
... The versions of for in these species affect the level of PKG The genes are orthologues - they descended from the same ancestral gene and have the same function But, for and PKG affects foraging through different mechanisms and may be regulated differently in species ...
... The versions of for in these species affect the level of PKG The genes are orthologues - they descended from the same ancestral gene and have the same function But, for and PKG affects foraging through different mechanisms and may be regulated differently in species ...
MayerFrankiPoster
... cyanobacterium Synechococcus sp. PCC 7002 to produce isoprene. However, for this ‘photo-isoprene’ to become a marketable option for replacing petroleum products, there are many more modifications to be made. One of these involves the gene cpcB that codes for a protein, called phycocyanin, that captu ...
... cyanobacterium Synechococcus sp. PCC 7002 to produce isoprene. However, for this ‘photo-isoprene’ to become a marketable option for replacing petroleum products, there are many more modifications to be made. One of these involves the gene cpcB that codes for a protein, called phycocyanin, that captu ...
Plasmids
... Plasmids Separate plasmid DNA from denatured proteins and chromosomal DNA 6. Add 350 µL of cold Yellow Zyppy Neutralization buffer (w/RNAase A) to the tube, and mix the contents thoroughly by inverting several times. The solution will turn yellow when neutralization is complete, and a yellowish pre ...
... Plasmids Separate plasmid DNA from denatured proteins and chromosomal DNA 6. Add 350 µL of cold Yellow Zyppy Neutralization buffer (w/RNAase A) to the tube, and mix the contents thoroughly by inverting several times. The solution will turn yellow when neutralization is complete, and a yellowish pre ...
Significant enhancement of fatty acid composition in seeds of the
... mutations in the FAD2 genes of Arabidopsis and Camelina plants was confirmed by DNA sequencing. Sequence analyses of DNA from leaf and seed samples (Data Set S2) confirmed multiple mutations over multiple generations at each of the three target sites in each of the three different FAD2 gene types pr ...
... mutations in the FAD2 genes of Arabidopsis and Camelina plants was confirmed by DNA sequencing. Sequence analyses of DNA from leaf and seed samples (Data Set S2) confirmed multiple mutations over multiple generations at each of the three target sites in each of the three different FAD2 gene types pr ...
reproductive cell fate transition in plants - Development
... KEY WORDS: Arabidopsis, Plant reproduction, Megaspore mother cell, Heterochromatin, Chromatin modifications, Histone variants ...
... KEY WORDS: Arabidopsis, Plant reproduction, Megaspore mother cell, Heterochromatin, Chromatin modifications, Histone variants ...
Replication origin plasticity, Taylor-made: inhibition vs
... investigators went on to inhibit the checkpoint-signaling pathway with caffeine, which is known to inhibit at least two critical mediators of the S-phase checkpoint cascade. This caused a dramatic increase in the density of newly fired replication origins, whether or not aphidicolin was present. App ...
... investigators went on to inhibit the checkpoint-signaling pathway with caffeine, which is known to inhibit at least two critical mediators of the S-phase checkpoint cascade. This caused a dramatic increase in the density of newly fired replication origins, whether or not aphidicolin was present. App ...
deschamp_2009_sequencing
... • Small genomes that are not too complex (microbial) • The longer the reads, the better – 454 chemistry most suitable • Paired-End sequencing ...
... • Small genomes that are not too complex (microbial) • The longer the reads, the better – 454 chemistry most suitable • Paired-End sequencing ...
Nonrandom cell-cycle timing of a somatic chromosomal
... ASPS. Individuals whose ASPS contains a balanced t(X;17) have not been found to be younger, on average, than those with an unbalanced t(X;17), ...
... ASPS. Individuals whose ASPS contains a balanced t(X;17) have not been found to be younger, on average, than those with an unbalanced t(X;17), ...
Analysis of a Rhizobium leguminosarum gene
... Helinski, 1979). Tnlac mutagenesis of pBI01000, which contains the gstA and gstR genes cloned in the wide-hostrange plasmid pLAFR1, was performed as follows. pBIOlOOO was transformed into E. coli strain A118, which contains a chromosomally located copy of Tnlac. The resultant strain was used as the ...
... Helinski, 1979). Tnlac mutagenesis of pBI01000, which contains the gstA and gstR genes cloned in the wide-hostrange plasmid pLAFR1, was performed as follows. pBIOlOOO was transformed into E. coli strain A118, which contains a chromosomally located copy of Tnlac. The resultant strain was used as the ...
PDF of article
... that was discovered some decades ago, but its functional role in eukaryotes still awaits elucidation. 5-Hydroxymethylcytosine is an epigenetic marker that is crucial for multiple biological processes. The profile is altered under certain disease conditions such as cancer, Huntington’s disease and Al ...
... that was discovered some decades ago, but its functional role in eukaryotes still awaits elucidation. 5-Hydroxymethylcytosine is an epigenetic marker that is crucial for multiple biological processes. The profile is altered under certain disease conditions such as cancer, Huntington’s disease and Al ...
X chromosome gene expression in human tissues
... somatic cell hybrid system), Nguyen and Disteche [16] found a significant increase in expression in females for only a few of these escaped genes. Because of a paucity of female to male gene expression ratios in the tissues reported by Nguyen and Disteche [16], we were unable to correlate our female ...
... somatic cell hybrid system), Nguyen and Disteche [16] found a significant increase in expression in females for only a few of these escaped genes. Because of a paucity of female to male gene expression ratios in the tissues reported by Nguyen and Disteche [16], we were unable to correlate our female ...
Gene Section CDA (Cytidine Deaminase) Atlas of Genetics and Cytogenetics
... On the other hand, controvertial results on the effects of activities have been obtained for 79A>C (Lys27Gln). The recombinant enzyme with Gln27 retained its catalytic activities for cytidine and ara-C as substrates (Yue et al., 2003), while showing reduced activity with increased Km value in the ca ...
... On the other hand, controvertial results on the effects of activities have been obtained for 79A>C (Lys27Gln). The recombinant enzyme with Gln27 retained its catalytic activities for cytidine and ara-C as substrates (Yue et al., 2003), while showing reduced activity with increased Km value in the ca ...
Identification of a NodD repressible gene
... In view of the observation that upstream of the nodFEL operon in R. leguminosarum bv. viciae there was a small divergently transcribed gene (px2), required for full induction of the nodFEL promoter [11], we analyzed the sequence upstream of nodM to determine whether there was an equivalent gene. We ...
... In view of the observation that upstream of the nodFEL operon in R. leguminosarum bv. viciae there was a small divergently transcribed gene (px2), required for full induction of the nodFEL promoter [11], we analyzed the sequence upstream of nodM to determine whether there was an equivalent gene. We ...
The role of variable DNA tandem repeats in bacterial adaptation
... cell division, energy production, and DNA replication and repair (Guo & Mrazek, 2008). Obviously, corresponding TR rearrangements leading to reading frame disruption are anticipated to be detrimental or even lethal for the cell, and it remains unclear why such TRs have been maintained during evolut ...
... cell division, energy production, and DNA replication and repair (Guo & Mrazek, 2008). Obviously, corresponding TR rearrangements leading to reading frame disruption are anticipated to be detrimental or even lethal for the cell, and it remains unclear why such TRs have been maintained during evolut ...
PDF
... pCR2.1-ACF1-flag using the Site-directed Mutagenesis Kit (Stratagene). The following pairs of oligonucleotides were used to delete the PHD1 or PHD2, respectively: PHD1F, 5⬘-ACCAATAAGTCATTAGTCGACGTAAAGAGTCTGGGTCTCAGC-3⬘; PHD1R, 5⬘-ACCCAGACTCTTTACGTCGACTAATGACTTATTGGTGGAACGC-3⬘; PHD2F, 5⬘-GATGAGGAAAAG ...
... pCR2.1-ACF1-flag using the Site-directed Mutagenesis Kit (Stratagene). The following pairs of oligonucleotides were used to delete the PHD1 or PHD2, respectively: PHD1F, 5⬘-ACCAATAAGTCATTAGTCGACGTAAAGAGTCTGGGTCTCAGC-3⬘; PHD1R, 5⬘-ACCCAGACTCTTTACGTCGACTAATGACTTATTGGTGGAACGC-3⬘; PHD2F, 5⬘-GATGAGGAAAAG ...
"Vectors in Gene Therapy". In: An Introduction to Molecular Medicine
... Based on the virus life cycle, infectious virions are very efficient at transferring genetic information. Most gene therapy experiments have used viral vectors comprising elements of a virus that result in a replication-incompetent virus. In initial studies, immediate or immediate early genes were d ...
... Based on the virus life cycle, infectious virions are very efficient at transferring genetic information. Most gene therapy experiments have used viral vectors comprising elements of a virus that result in a replication-incompetent virus. In initial studies, immediate or immediate early genes were d ...
number of fifty human tumours
... cells are measured, it is nearly always found that the majority are grouped together, although the level of the mode varies widely in different tumours. A few cells may be expected to be synthesising DNA and to have values up to twice the "resting " value for this reason, but clearly these usually f ...
... cells are measured, it is nearly always found that the majority are grouped together, although the level of the mode varies widely in different tumours. A few cells may be expected to be synthesising DNA and to have values up to twice the "resting " value for this reason, but clearly these usually f ...
Cancer epigenetics
Cancer epigenetics is the study of epigenetic modifications to the genome of cancer cells that do not involve a change in the nucleotide sequence. Epigenetic alterations are as important as genetic mutations in a cell’s transformation to cancer, and their manipulation holds great promise for cancer prevention, detection, and therapy. In different types of cancer, a variety of epigenetic mechanisms can be perturbed, such as silencing of tumor suppressor genes and activation of oncogenes by altered CpG island methylation patterns, histone modifications, and dysregulation of DNA binding proteins. Several medications which have epigenetic impact are now used in several of these diseases.