Gene Expression Microarray Analysis of Archival FFPE Samples
... indicated that the RNA samples were free of genomic DNA contaminants. RNA amplification and cDNA labeling Amplified cDNA was generated with the the TransPlex Whole Transcriptome Amplification system (Sigma P/N WTA150RXN). An input mass of 50 ng from both the FFPE-derived total RNA and from the fresh ...
... indicated that the RNA samples were free of genomic DNA contaminants. RNA amplification and cDNA labeling Amplified cDNA was generated with the the TransPlex Whole Transcriptome Amplification system (Sigma P/N WTA150RXN). An input mass of 50 ng from both the FFPE-derived total RNA and from the fresh ...
PDF
... differential genetic regulation of developing floral buds identified at meiosis between autotetraploid and diploid B. rapa. In total, compared to its diploid counterparts, among all 40,927 expressed genes revealed, 4,601 differentially expressed genes (DEGs) were identified in the floral buds of aut ...
... differential genetic regulation of developing floral buds identified at meiosis between autotetraploid and diploid B. rapa. In total, compared to its diploid counterparts, among all 40,927 expressed genes revealed, 4,601 differentially expressed genes (DEGs) were identified in the floral buds of aut ...
CHK2 kinase: cancer susceptibility and cancer therapy – two sides
... response. As can be seen in FIG. 1, CHK2 and CHK1 share a number of overlapping substrates, leading to the initial proposal that they could be functionally redundant. It is now clear, however, that they have distinct roles in directing the cell’s response to DNA damage (BOX 2) . The response of CHK2 ...
... response. As can be seen in FIG. 1, CHK2 and CHK1 share a number of overlapping substrates, leading to the initial proposal that they could be functionally redundant. It is now clear, however, that they have distinct roles in directing the cell’s response to DNA damage (BOX 2) . The response of CHK2 ...
Gene Section HFE (hemochromatosis) Atlas of Genetics and Cytogenetics in Oncology and Haematology
... is associated with increased risk to childhood acute lympho-blastic leukemia in boys only. This association has not been noted in other studies in Finland, Spain and Mexico. In Italy, adult leukemia shows an association with H63D mutation. ...
... is associated with increased risk to childhood acute lympho-blastic leukemia in boys only. This association has not been noted in other studies in Finland, Spain and Mexico. In Italy, adult leukemia shows an association with H63D mutation. ...
Cloning and Genetic Analysis of Six Pyrroloquinoline
... to oxidize glucose to gluconate (Goosen et al., 1987). These mutants were used for cloning four genes involved in PQQ biosynthesis. DNA sequence analysis failed to give clues to the nature of putative biosynthesis enzymes (Goosen et al., 1989). In some facultative methylotrophic bacteria such as M. ...
... to oxidize glucose to gluconate (Goosen et al., 1987). These mutants were used for cloning four genes involved in PQQ biosynthesis. DNA sequence analysis failed to give clues to the nature of putative biosynthesis enzymes (Goosen et al., 1989). In some facultative methylotrophic bacteria such as M. ...
Two enhancer regions in the mouse En-2 locus
... CNS, En-2 is expressed in cells surrounding the developing pituitary (Davis et al., 1988, 1991). In addition, immunohistochemical analysis using a polyclonal antiserum (aEnhb-1) that detects both En-1 and En-2 protein has shown that one or both genes are expressed in presumptive myoblasts within the ...
... CNS, En-2 is expressed in cells surrounding the developing pituitary (Davis et al., 1988, 1991). In addition, immunohistochemical analysis using a polyclonal antiserum (aEnhb-1) that detects both En-1 and En-2 protein has shown that one or both genes are expressed in presumptive myoblasts within the ...
Special Feature —Manipulating Genes to Understand
... advances in our understanding of the mechanisms of gene expression and regulation and the development of animal models of human diseases are but two examples of how this technology has affected medical science. Transgenic animals are defined as animals in which a segment of DNA has been physically i ...
... advances in our understanding of the mechanisms of gene expression and regulation and the development of animal models of human diseases are but two examples of how this technology has affected medical science. Transgenic animals are defined as animals in which a segment of DNA has been physically i ...
Chapter 8
... Post-Transcriptional Control • microRNAs (miRNAs) base pair with mRNA to make it double-stranded • Double-stranded RNA is enzymatically destroyed, preventing production of a protein ...
... Post-Transcriptional Control • microRNAs (miRNAs) base pair with mRNA to make it double-stranded • Double-stranded RNA is enzymatically destroyed, preventing production of a protein ...
Aberrant replication timing induces defective chromosome
... The origin recognition complex (ORC) is composed of six subunits, ORC1–6 [1]. ORCs have been identified in yeast, flies, frogs, mice and humans [2], and several of the homologous subunits or even whole complexes are functionally interchangeable between species, suggesting a high degree of conservati ...
... The origin recognition complex (ORC) is composed of six subunits, ORC1–6 [1]. ORCs have been identified in yeast, flies, frogs, mice and humans [2], and several of the homologous subunits or even whole complexes are functionally interchangeable between species, suggesting a high degree of conservati ...
Genetics of Colorectal Cancer - Scioto County Medical Society
... 60-70% right-sided/proximal CRC tumors Polyps may be present, multiple primaries common. Can overlap with AFAP. ...
... 60-70% right-sided/proximal CRC tumors Polyps may be present, multiple primaries common. Can overlap with AFAP. ...
lecture - Berkeley MCB
... What democracy, I mean, gene regulation, is really like • Trans-acting factors do not distribute in the nucleus based on the primary sequence of the genome: some factors fail to bind most genes that have sequences waiting for them, and other factors bind a large number of genes that do NOT have seq ...
... What democracy, I mean, gene regulation, is really like • Trans-acting factors do not distribute in the nucleus based on the primary sequence of the genome: some factors fail to bind most genes that have sequences waiting for them, and other factors bind a large number of genes that do NOT have seq ...
Diversity of DNA methyltransferases that recognize asymmetric
... DNA methyltransferases (MTases) are a group of enzymes that catalyze the methyl group transfer from S-adenosyl-L-methionine in a sequence-specific manner. Orthodox Type II DNA MTases usually recognize palindromic DNA sequences and add a methyl group to the target base (either adenine or cytosine) on ...
... DNA methyltransferases (MTases) are a group of enzymes that catalyze the methyl group transfer from S-adenosyl-L-methionine in a sequence-specific manner. Orthodox Type II DNA MTases usually recognize palindromic DNA sequences and add a methyl group to the target base (either adenine or cytosine) on ...
Recent retrotransposition events have not affected
... The upstream bias is just significant (p = 0.049). TEs may accumulate upstream because they have a role in chromatin regulation. (Huda et al, Gene, 2009) TEs may preferentially insert upstream (as known for Drosophila P elements) ...
... The upstream bias is just significant (p = 0.049). TEs may accumulate upstream because they have a role in chromatin regulation. (Huda et al, Gene, 2009) TEs may preferentially insert upstream (as known for Drosophila P elements) ...
Molecular Genetic Testing For BRAF Mutations
... For BRAF Mutations Tests Available: • BRAF V600E by real-time PCR • BRAF (V600E) mutation only by Sanger sequencing • BRAF full gene sequence analysis ...
... For BRAF Mutations Tests Available: • BRAF V600E by real-time PCR • BRAF (V600E) mutation only by Sanger sequencing • BRAF full gene sequence analysis ...
PcrA Helicase Tightly Couples ATP Hydrolysis to Unwinding Double
... the duplex separation around the plasmid. In contrast, the lagging strand synthesis is initiated from a separate singlestranded origin, which only becomes available once the duplex separation has gone past this origin (12). The plasmid dso sequence has several inverted complementary repeats (ICRs) i ...
... the duplex separation around the plasmid. In contrast, the lagging strand synthesis is initiated from a separate singlestranded origin, which only becomes available once the duplex separation has gone past this origin (12). The plasmid dso sequence has several inverted complementary repeats (ICRs) i ...
PTC Receptor Project Lab Protocol
... and/or function of the PTC receptor, leading to a nontaster phenotype in most AVI homozygotes. A non-taster phenotype may be because (a) the PTC receptor is not present in the plasma membranes of the taste receptors in non-tasters; (b) the non-taster PTC receptor may not bind PTC with the same affin ...
... and/or function of the PTC receptor, leading to a nontaster phenotype in most AVI homozygotes. A non-taster phenotype may be because (a) the PTC receptor is not present in the plasma membranes of the taste receptors in non-tasters; (b) the non-taster PTC receptor may not bind PTC with the same affin ...
Pearson science 10 Teaching Program 3–4 weeks Chapter 1 DNA
... In this chapter, students will learn about DNA and be able to describe its role in controlling the characteristics of organisms. They will use models and diagrams to represent relationships between DNA, genes and chromosomes. They will be able to explain the role of meiosis and fertilisation in the ...
... In this chapter, students will learn about DNA and be able to describe its role in controlling the characteristics of organisms. They will use models and diagrams to represent relationships between DNA, genes and chromosomes. They will be able to explain the role of meiosis and fertilisation in the ...
A Tn 10-lacZ-kanR-URA3 Gene Fusion Transposon for Insertion Mutagenesis and Fusion Analysis of Yeast and Bacterial Genes.
... If no screen for the yeast gene phenotype is available in E. coli, inserts into yeast DNA can be identified in any of three ways: 1. If inserts both inside and outside the gene of interest are desired, the plasmids present in the KanR AmpR (Tets)transformants from the DNA pools can be individually c ...
... If no screen for the yeast gene phenotype is available in E. coli, inserts into yeast DNA can be identified in any of three ways: 1. If inserts both inside and outside the gene of interest are desired, the plasmids present in the KanR AmpR (Tets)transformants from the DNA pools can be individually c ...
10709_2015_9875_MOESM4_ESM
... Deutschbauer et al. (2005) was the first group to analyze haploinsufficiency in S. cerevisiae. Thus, we repeated our key chemogenetic and GI network analyses with datasets from this study. Unfortunately, the results based on the Deutschbauer et al. datasets are not in agreement with the results obs ...
... Deutschbauer et al. (2005) was the first group to analyze haploinsufficiency in S. cerevisiae. Thus, we repeated our key chemogenetic and GI network analyses with datasets from this study. Unfortunately, the results based on the Deutschbauer et al. datasets are not in agreement with the results obs ...
Recurrent patterns of DNA copy number alterations in tumors reflect
... and pentose phosphate pathway were most predictive..."; p. 14: "p53-/- cells did not undergo senescence and exhibited less strong copy-number alterations than wild-type signature A MEFs" these statements need to be omitted if no significant p-value can be provided, or alternatively included with the ...
... and pentose phosphate pathway were most predictive..."; p. 14: "p53-/- cells did not undergo senescence and exhibited less strong copy-number alterations than wild-type signature A MEFs" these statements need to be omitted if no significant p-value can be provided, or alternatively included with the ...
10 Day Lesson Plan - Joseph L. Anderson
... what does it all mean and why RNA? During cell reproduction we have found that the DNA unwinds and separates. It has also been learned that two identical DNA strands are formed for each daughter cell to have all the information it needs to carry out cell functions. RNA plays a critical role in prote ...
... what does it all mean and why RNA? During cell reproduction we have found that the DNA unwinds and separates. It has also been learned that two identical DNA strands are formed for each daughter cell to have all the information it needs to carry out cell functions. RNA plays a critical role in prote ...
beckwith-wiedemann syndrome
... (UPD) which occurs when both copies of chromosome 11 are derived from the same parent. Patients with BWS and paternal UPD have two paternally derived copies of 11p15.5 and no maternal contribution for this region. In these patients, there are methylation defects at both KvDMR1 and H19. ...
... (UPD) which occurs when both copies of chromosome 11 are derived from the same parent. Patients with BWS and paternal UPD have two paternally derived copies of 11p15.5 and no maternal contribution for this region. In these patients, there are methylation defects at both KvDMR1 and H19. ...
Solid Tumour Section Esophagus: Barrett's esophagus, dysplasia and adenocarcinoma
... regulation and in DNA damage repair. p53 transcriptionally regulates multiple genes functioning as an inhibitor of cell growth and proliferation and inducer of apoptosis. Loss of TP53 functions promotes tumor progression, most likely by preventing cell cycle arrest, suppressing apoptosis and permitt ...
... regulation and in DNA damage repair. p53 transcriptionally regulates multiple genes functioning as an inhibitor of cell growth and proliferation and inducer of apoptosis. Loss of TP53 functions promotes tumor progression, most likely by preventing cell cycle arrest, suppressing apoptosis and permitt ...
Cancer epigenetics
Cancer epigenetics is the study of epigenetic modifications to the genome of cancer cells that do not involve a change in the nucleotide sequence. Epigenetic alterations are as important as genetic mutations in a cell’s transformation to cancer, and their manipulation holds great promise for cancer prevention, detection, and therapy. In different types of cancer, a variety of epigenetic mechanisms can be perturbed, such as silencing of tumor suppressor genes and activation of oncogenes by altered CpG island methylation patterns, histone modifications, and dysregulation of DNA binding proteins. Several medications which have epigenetic impact are now used in several of these diseases.