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The nuclear envelope in genome organization, expression and
The nuclear envelope in genome organization, expression and

... DNA adenine methyltransferase identification (DamID), which can detect loci located near the nuclear periphery by identifying DNA that becomes methylated following the expression of the Escherichia coli Dam methylase fused to a protein located at the nuclear periphery, such as an INM or lamin protei ...
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word

... interested in cell growth and proliferation patterns during seed development and how the different parts that comprise a seed coordinate their development. Research from many groups has shown that epigenetic regulation plays an import role during seed development. Among other epigenetic mechanisms i ...
Giant chromosomes
Giant chromosomes

... A general chromosome model • A general chromosome model based on these studies has been proposed in which loops of 30-nm fibers extend at an angle from the main axis of the chromosome ...
The past, present and future of plant breeding
The past, present and future of plant breeding

... is only one of the many possibilities we have to make plants respond better to our needs. In this VIB Fact series issue, we outline how the crops we know today have evolved from nature, with particular emphasis on the role humans have played. Since agriculture began around 10,000 years ago, humans h ...
Additional file 4 - Springer Static Content Server
Additional file 4 - Springer Static Content Server

... nucleotide polymorphic sites. This diploid cell fails to divide resulting in a tetraploid cell with a 92,XXXX or 92,XXYY karyotype and AAAA, AABB, or BBBB single nucleotide polymorphic sites. All tetraploid results explain fetal demise. [49,50,51] FISH and karyotyping readily distinguish these categ ...
Plant Telomere Biology
Plant Telomere Biology

... Peter Baumann and Tom Cech identified homologs from multicellular eukaryotes, including Arabidopsis (Baumann and Cech, 2001; Baumann et al., 2002). This delay was a consequence of the poor sequence conservation of single-strand binding telomere proteins: only with the advent of genome sequencing pro ...
Practical General Microbiology Lab
Practical General Microbiology Lab

... The 7.5% concentration of sodium chloride results in the inhibition of bacterial organisms other than staphylococci. Mannitol fermentation, as indicated by a change in the phenol red indicator, aids in the differentiation of staphylococcal species. ...
Identification of Vietnamese Coptotermes pest species based on the
Identification of Vietnamese Coptotermes pest species based on the

... Coptotermes, found in urban areas, is regarded as the most abundant building termite pest genus, widely distributed in Vietnam. The objectives of this study were to classify the Coptotermes found in certain provinces in Vietnam and assess the feasibility proposed PCR method by Szalanski et al., 2004 ...
19.1 CONSTITUTIVE, INDUCIBLE AND REPRESSIBLE GENE
19.1 CONSTITUTIVE, INDUCIBLE AND REPRESSIBLE GENE

... The Lac promoter is intrinsically "weak" because of a poor match to consensus sequence of the sigma factor. This is important for positive regulation by CAP. The mechanism by which glucose regulates adenyl cyclase activity in this case is not yet known. It is interesting to note that cAMP is found a ...
RECOMBINEERING: A POWERFUL NEW TOOL FOR MOUSE
RECOMBINEERING: A POWERFUL NEW TOOL FOR MOUSE

... genome will be completed and annotated. The next challenge will be to determine how each of these genes functions alone and with other genes in the genome, to understand the developmental programme of a human. Given that there are many genes that need to be characterized and the fact that a lot of t ...
Complete Laboratory PDF
Complete Laboratory PDF

... with a number of previously mapped genes or DNA markers. Linkage is the principle that the closer two genes or markers are located to one another on a chromosome, the greater the chance that they will be inherited together as a unit (linked). Conversely, locations farther apart on the chromosome are ...
Genes Practice Questions
Genes Practice Questions

... 46 If you were to sequence the DNA of a housefly, what bases would you find in its genome? ...
KAIE LOKK Comparative genome-wide DNA methylation
KAIE LOKK Comparative genome-wide DNA methylation

... correlations, tissue-specific differentially methylated regions have been increasingly found among intragenic regions, such as in gene bodies or even in intergenic regions. But the relationship between DNA methylation in nonpromoter regions and gene expression remains unclear. Cancer, especially lun ...
Genes: Structure, Replication, and Mutation
Genes: Structure, Replication, and Mutation

... 7. Most bacterial genes have at least four major parts, each with different functions: promoters, leaders, coding regions, and trailers. 8. Mutations are stable, heritable alterations in the gene sequence and usually, but not always, produce phenotypic changes. Nucleic acids are altered in several d ...
GCAT-SEEK Workshop - Prokaryotic Genomics Module – Jeff
GCAT-SEEK Workshop - Prokaryotic Genomics Module – Jeff

... The 16S rRNA gene is present in all Bacteria and Archaea. Certain sequences within the gene have not changed much in billions of years due to their essential nature for the function of the 16S rRNA gene product. These conserved sequences can be used as primer annealing sites to amplify the 16S rRNA ...
SNP Analysis of the PTC Gene Using PCR
SNP Analysis of the PTC Gene Using PCR

... base pair has been deleted or added to a sequence. SNPs are the most common type of genetic variation among people. They occur more frequently in the non-coding regions of genes and in regions between genes. Although these SNPs do not automatically translate into amino acids they may still affect pr ...
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... reported within P. ramorum clonal lineages [10] and these data on P. infestans will be reported in more detail in subsequent publications. Isolates with novel combinations of alleles across several loci and found at a very low frequency and commonly in only a single blight outbreak were grouped into ...
Non-Mendelian Inheritance
Non-Mendelian Inheritance

... maternal transmission of cpDNA is governed by a methylation-restriction system analogous to that found in bacteria: after gametic fusion, the mt– cpDNA is digested by a restriction enzyme while the modified mt+ cpDNA remains unaffected. Modification is assumed to occur as a result of methylation. St ...
The Making of the Fittest: Evolving Switches, Evolving Bodies
The Making of the Fittest: Evolving Switches, Evolving Bodies

... Key Points to Emphasize: • Students may wonder why Pitx1 is expressed in such diverse tissues. This is because the Pitx1 gene contains multiple regulatory switches that allow for transcription of that gene in multiple tissues. The expression of Pitx1 is important in various tissues because the Pitx1 ...
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... transposon-based vectors remains the most suitable gene-delivery system for producing transgenic insects. The most common in current use are vectors based on P- element piggyBac, mariner, Minos, hobo and Hermes [4,7]. Such standard nonautonomous vectors contain target DNA surrounded by transposon se ...
Understanding Our Environment
Understanding Our Environment

... Expression of Information  Different subset of the genetic information is read in each cell type.  Expression requires two processes. - Transcription - Copy of gene message using RNA. - Translation  Messenger RNA (mRNA) translated to produce proteins. Stern - Introductory Plant Biology: 9th Ed. - ...
University of Bucharest, Faculty of Biology, Molecular Biology Center
University of Bucharest, Faculty of Biology, Molecular Biology Center

... The molecular basis of BLAD is a single point mutation (A-G) at position 383 in the cDNA of the CD18 gene. This mutation results in a substitution of a glycine for an aspartic acid at position 128 in the D128G protein [2; 5; 8; 11; 15]. Viana [16] and Shuster [15] also described the existence of the ...
SVD and PCA
SVD and PCA

... • Images under different illumination • Most variation captured by first 5 principal components – can re-illuminate by combining only a few images ...
Electrophoresis Systems for Nucleic Acids
Electrophoresis Systems for Nucleic Acids

... When nucleic acid (DNA/RNA) is dissolved in buffer solution, etc. it has a negative charge. When this solution (sample) is added to agarose gel and electrophoresed in buffer solution for a regulated time, the level of migration shown is in accordance with the size of nucleic acid due to the molecula ...
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+ 2 subjective question bank reproduction

... 1. Name the enzyme and state its property that is responsible for continuous and discontinuous replication of the two strands of a DNA molecule. Ans. DNA-dependent. DNA polymerase is an enzyme involved in template directed synthesis of DNA from deoxyribonucleotide triphosphates. This enzyme has the ...
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Epigenomics

Epigenomics is the study of the complete set of epigenetic modifications on the genetic material of a cell, known as the epigenome. The field is analogous to genomics and proteomics, which are the study of the genome and proteome of a cell (Russell 2010 p. 217 & 230). Epigenetic modifications are reversible modifications on a cell’s DNA or histones that affect gene expression without altering the DNA sequence (Russell 2010 p. 475). Two of the most characterized epigenetic modifications are DNA methylation and histone modification. Epigenetic modifications play an important role in gene expression and regulation, and are involved in numerous cellular processes such as in differentiation/development and tumorigenesis (Russell 2010 p. 597). The study of epigenetics on a global level has been made possible only recently through the adaptation of genomic high-throughput assays (Laird 2010) and.
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