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Level 2 Biology - No Brain Too Small
Level 2 Biology - No Brain Too Small

... Demonstrate in-depth understanding involves providing reasons as to how or why genetic variation and change occurs. Demonstrate comprehensive understanding involves linking biological ideas about genetic variation and change. The discussion of ideas may involve justifying, relating, evaluating, comp ...
double core - MG University
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... 22. In vitro mutagenesis 23. Nick translation 24. Homopolymer tailing Part C (Answer any 4- weight 2 each) 26. What are the advantages of using a restriction enzyme with relatively few cutting sites? When would you use such enzymes? 25. The human insulin gene contains a number of introns. In spite o ...
Level 2 Biology - No Brain Too Small
Level 2 Biology - No Brain Too Small

... Demonstrate in-depth understanding involves providing reasons as to how or why genetic variation and change occurs. Demonstrate comprehensive understanding involves linking biological ideas about genetic variation and change. The discussion of ideas may involve justifying, relating, evaluating, comp ...
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... Gateway® cloning system: general overview - Gateway technology (Life Technologies) is a cloning system based on the recombinational properties of lambda phage in Escherichia coli, where it alternates between lytic and lysogenic cycle. This recombination occurs between the attachment (att) sites pres ...
Gene therapy should be used only for the treatment of serious disease
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... Premise: Medicine is an inexact science Premise: We have only limited understanding of how the human body works – disassembling the clock example Premise: It’s harder to make an improvement than to repair existing problems according to the existing design. – disassembling the clock example Premise: ...
Mitosis and Cell Division
Mitosis and Cell Division

... • Gene: Segment of DNA that represents all information for a product as well as when and where to make the product • Allele: A version (or flavor) of a gene; two alleles of the same gene my differ by a nucleotide or dozens of them--generally a small number • Dominant/recessive: Two alleles enter; on ...
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... Breast cancer kills more women than it does men, but it is a question that faces all in society. This chapter aims to consider: 1. genetic testing using the example of breast cancer. 2. risks and benefits of genetic testing. 3. limitations of genetic testing. C4.1. Testing for cancer gene susceptibi ...
Activating the MSH2/MSH6 Apoptotic Pathway in Cancer Cells
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... signaling” hypothesis propounds a dual functionality for at least the MutSα complex in eukaryotes. According to this hypothesis, cell death is initiated by the MMR proteins themselves, particularly MutSα, through direct signaling that results in the activation of the caspase-mediated apoptotic-signa ...
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Practical Guide: Selecting the Optimal Resins for Removal of DNA
Practical Guide: Selecting the Optimal Resins for Removal of DNA

... purification steps such as anion exchange chromatography. In addition, contamination with cellular DNA creates a therapeutic risk. Regulatory authorities require that DNA levels in all therapeutic protein and antibody samples be reduced to 10–100 pg/dose. Cell culture clarification processes, such a ...
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mg-lecure1 - WordPress.com

... – In most animal cells, about 5% of the cytosine bases are methylated – More than 50% of the cytosine bases in some plants are methylated – No methylation of cytosine has been detected in yeast cells – very low levels of methylation (about 1 methylated cytosine base per 12,500 nucleotides) are found ...
A Physiological Approach to DNA Music
A Physiological Approach to DNA Music

... 1. Introduction: DNA and RNA With the exception of Prions, all known life forms on the planet use nucleic acid molecules (either DNA or RNA) to store genetic information. In eukaryotes, protozoans, yeast, and bacteria, the genetic material is invariably DNA, whereas some viruses use RNA as their gen ...
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Vectors in gene therapy

Gene therapy utilizes the delivery of DNA into cells, which can be accomplished by several methods, summarized below. The two major classes of methods are those that use recombinant viruses (sometimes called biological nanoparticles or viral vectors) and those that use naked DNA or DNA complexes (non-viral methods).
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