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Epigenomics Workshop - Institute for Systems Genomics

... Environment)  has  strong  expertise  in  stress-responsive  TFs   (HSFs),   and   a   leading   position   in   the   transcriptomic   and   epigenetic   mechanisms   underlying   the   stress   response   of   the  fetal  brain  (collab.  P. ...
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... directs a Cas9 nuclease to a specific genomic locus. Cas9 cleaves the gene of interest and creates a double­ stranded break (DSB) in the DNA, which can be repaired by non­homologous end joining (NHEJ). As NHEJ is an error­prone DNA repair process, insertions and deletions (indels) are often introduc ...
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Vectors in gene therapy

Gene therapy utilizes the delivery of DNA into cells, which can be accomplished by several methods, summarized below. The two major classes of methods are those that use recombinant viruses (sometimes called biological nanoparticles or viral vectors) and those that use naked DNA or DNA complexes (non-viral methods).
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