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ChIP-seq
ChIP-seq

... •Identifying genes and annotating regulatory function within and among genomes •Computational issues: data normalization, peak calling, differential expression and binding •Large-scale studies revealing regulatory architecture of human & model genomes ...
UAB DIVISION of Molecular and Cellular Pathology
UAB DIVISION of Molecular and Cellular Pathology

Functional analysis of the regulatory region of a zein gene in
Functional analysis of the regulatory region of a zein gene in

... ment 2 and may suggest an endosperm-specific function for the 7-11-7 motif. Accordingly, Quayle etal. [23] have recently reported the enhancement of the 35S CaMV promoter by the -300 box in protoplasts from an endosperm culture. In our endosperm culture protoplasts, GUS activity is driven only by th ...
A-level Human Biology Question Paper Unit 04 - Bodies and
A-level Human Biology Question Paper Unit 04 - Bodies and

... The table shows the base sequence in part of one of the strands of a DNA molecule. Complete the table to show the base sequence in the mRNA that is transcribed from this DNA sequence. Base sequence in DNA ...
Applications of site-specific recombination As can be
Applications of site-specific recombination As can be

... A potentially useful approach to expand the utility of site-specific recombination is to generate recombinases with altered binding (DNA recognition) specificities. That is, change the sequence of the binding elements, and then produce active recombinase variants that have acquired the corresponding ...
Seed specific polycomb group gene and methods of use for same
Seed specific polycomb group gene and methods of use for same

... pseudogamy or fertiliZation of the polar nuclei to produce endosperm is necessary for seed viability. These types of apomixis have economic potential because they can cause any genotype, regardless of hoW heterozygous, to breed true. It is a reproductive process that bypasses female meiosis and syng ...
DNA Technology Notes
DNA Technology Notes

B1.7 Genes - Pearson Schools and FE Colleges
B1.7 Genes - Pearson Schools and FE Colleges

... Scientists think that bats evolved from mouse-like animals about 50 million years ago. They think that a group of these animals suddenly got very long ‘fingers’, which they started to use as wings. The sudden change was caused by a mistake in the ‘instructions’ inside cells. Three of the main parts ...
Genit 7
Genit 7

... Info comes from DNA which can replicate and duplicate itself. Then DNA transcription to produce mRNA which is translated into amino acids that produce proteins. Sometimes we have reverse transcription condition. Slide 5 Each step in protein synthesis there is a gene controlling that step. e.g: we ha ...
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viral infection

... • obligate intracellular parasites ( lacks ribosomes ...
DNA Technology Notes (13.1 &13.2)
DNA Technology Notes (13.1 &13.2)

... Which type of protein can recognize specific DNA sequences and cleave the DNA within that sequence? ...
STAAR Review 3
STAAR Review 3

... body. 2. RNA molecules use instruction from DNA to assemble proteins. There are three types of RNA molecules: mRNA, rRNA and tRNA. What specific function does mRNA perform in the process of making proteins? a. It brings instructions from DNA in the cell nucleus to the cytoplasm. b. It clamps onto me ...
Mutations KEY File
Mutations KEY File

... are malaria bearing mosquitoes because it protects people who carry it against malaria. People who carried the gene would survive and reproduce, passing the gene on. There is no benefit to sickle cell anemia in regions that don’t have malaria bearing mosquitoes, so there would be a lower incidence o ...
Conclusion Introduction Background The PTC Sensitivity Gene
Conclusion Introduction Background The PTC Sensitivity Gene

... Individuals’ DNA that are homozygous for the taster or nontaster alleles will only be amplified either with taster or nontaster specific primers, respectively. DNA of people who are heterozygous for the taster/nontaster alleles will have amplification products in both PCR reactions (Figure 2). Thus ...
Topic 3.2: Carbohydrates, Lipids, and Proteins
Topic 3.2: Carbohydrates, Lipids, and Proteins

T cell Metabolism–Regulating Energy
T cell Metabolism–Regulating Energy

... oxidative phosphorylation. In resting T cells oxidative phosphorylation was described to be the central energy producing process [1,2]. Furthermore it was described that upon activation the energy produced by oxidative phosphorylation in resting cells is not sufficient. Therefore lymphocytes undergo ...
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An Introduction to Genetic Analysis Chapter 14 Genomics Chapter
An Introduction to Genetic Analysis Chapter 14 Genomics Chapter

... nature of whole genomes; and functional genomics, characterizing the transcriptome (the entire range of transcripts produced by a given organism) and the proteome (the entire array of encoded proteins). The prime directive of structural genomic analysis is the complete and accurate elucidation of th ...
Mitosis
Mitosis

... Humans have two sets of chromosomes, one inherited from each parent.  Diploid Cells: Cells whose nuclei contain two homologous sets of chromosomes (2n). ...
ch11_lecture
ch11_lecture

... • A piece of one chromosome becomes attached to another nonhomologous chromosome • Most are reciprocal • Philadelphia chromosome arose from a reciprocal translocation between chromosomes 9 and 22 ...
You Light Up My Life
You Light Up My Life

... • A piece of one chromosome becomes attached to another nonhomologous chromosome • Most are reciprocal • Philadelphia chromosome arose from a reciprocal translocation between chromosomes 9 and 22 ...
Chapter 8: Mitosis - Cell Division and Reproduction
Chapter 8: Mitosis - Cell Division and Reproduction

... Humans have two sets of chromosomes, one inherited from each parent.  Diploid Cells: Cells whose nuclei contain two homologous sets of chromosomes (2n). ...
1. Explain what is meant by the “modern synthesis”.
1. Explain what is meant by the “modern synthesis”.

... may confer no selective advantage or disadvantage  variation in DNA which does not code for proteins may be nonadaptive  ...
Competency in Mismatch Repair Prohibits Clonal Expansion of
Competency in Mismatch Repair Prohibits Clonal Expansion of

... causing G to A transitions (2, 10). Correction of O6-meG-containing base pairs has been demonstrated in Escherichia coli, when wild-type levels of mutL and mutS MMR proteins are present (19). Recently, the protein complex encoded by hMSH2 and GTBP (termed hMutSa) has been shown to bind to nucleotide ...
Cas9 Protein Product Analysis Certificate
Cas9 Protein Product Analysis Certificate

... Fig 1. HDR rescue efficiency of donor EGFP fragment for Cas9 protein + gRNA system (right) compared with all-inone Cas9 plasmid system (left) as measured by GFP positive clones at day 3 post-transfection into cell line with mutant EGFP gene (EGIP). ...
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Vectors in gene therapy

Gene therapy utilizes the delivery of DNA into cells, which can be accomplished by several methods, summarized below. The two major classes of methods are those that use recombinant viruses (sometimes called biological nanoparticles or viral vectors) and those that use naked DNA or DNA complexes (non-viral methods).
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