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Sensitive and Sequence-Specific DNA Assays
Sensitive and Sequence-Specific DNA Assays

... SPR (SPR) has been demonstrated as a powerful technique for rapid, sensitive, and label-free genetic analysis [1-5]. When the sensor surface is coated with a single sensing (probe) DNA, SPR can be used for both affinity binding studies (i.e., kinetic measurements) and concentration detection of a ta ...
Depth-stratified functional and taxonomic niche
Depth-stratified functional and taxonomic niche

... evolve under different selective pressures than their host versions and have recombined back into the host (Sullivan et al., 2006). Cyanophage AMGs can also evolve to the point that they perform modified function. For example, when discovered, viral pebS was most similar to a cyanobacterial pebA gen ...
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... Base your answer(s) to the following question(s) on the diagram below, which represents some biochemical reactions involved in a cellular process, and on your knowledge of biology. ...
Improving coverage of poorly sequenced regions in clinical exomes
Improving coverage of poorly sequenced regions in clinical exomes

... sensitivity, there are still poorly covered regions that remain and may result in missed pathogenic variants. To minimize this problem, we have designed new sets of primers for low coverage AmpliSeq amplicons and amplified these independently at lower multiplicity than the highly multiplexed standar ...
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cell division - Bakersfield College
cell division - Bakersfield College

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... Mutations are passed on when cells divide. Many are insignificant. However, some produce new traits in the new cells or in offspring. These new traits usually produce harmful effects. They result in disease or even death. But on rare occasions, mutations produce beneficial new traits. These may enab ...
Sickle Cell Crisis
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... present, and the hypoxia responds poorly to inhaled oxygen. Crackles are often present in the lung bases and will ascend rapidly to involve the whole lung fields in severe cases. Radiological changes follow late and patients may be critically ill with near normal radiology. If a chest crisis is susp ...
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fontanes et al.indd - RiuNet

... Several other coat colour loci remain to be characterised at DNA level in this species. Among them, the dilute locus is determined by a recessive coat colour mutation that modifies the basic colours influenced by the agouti and extension mutations. This allele dilutes the black to blue (grey) and th ...
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... human cells emit green fluorescent light. This is done by using the gene of green fluorescent protein (GFP) from jellyfish. We have prepared the gene for you to work with, consisting of a double stranded piece of DNA. You will also receive a flask of living mammalian cells. In order to get the gene ...
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... same in many subjects. Two common methods: • Global: All genes in an array are multiplied by the ratio of the (global) mean over all genes for all arrays to the mean over all genes for this array. E.g., array1 has mean 1000 and fourteen arrays have mean 900, multiply by 0.90. • Z-score: Replace expr ...
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... The Virology Laboratory at St. Louis Children’s Hospital offers a full range of testing for common viral agents isolated from adult and pediatric patients. Cell culture and shell-vial assays are the mainstay of offered tests. Specimens are received, processed, and inoculated into a variety of cell c ...
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... UGA UAA or UAG, and which therefore causes an incomplete polypeptide to be released from the ribosome. A mutation in one of the sequences that controls gene expression rather than the coding sequence itself. These may include promoters, polyadenylation sequences or gene splicing sites. Severity of a ...
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... Deduce important motifs (for example in gene expression) Useful for industry application and fighting yeast pathogens ...
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The Founder Effect and Deleterious Genes - Deep Blue

... fact that the entire French Canadian population stems from about 10,000 original settlers (Henripin, ’54) may have led to this population having its own set of lethal genes. The high frequencies of deleterious genes in the Eastern European Jewish populations of Lithuania and Eastern Poland may have ...
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A Molecular Profile of the Malignant Transformation of Plasma Cells

... patients (PCs taken 24 hr or 48 hr after initial sample). All samples from the 12 patients studied longitudinally were found to cluster adjacent to one another. This indicated that gene expression in samples from the same patient were more similar to each other than they were to all other samples (d ...
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Power, Sex, Suicide. Mitochondria and the Meaning

... electron acceptor - occur at fixed frequency. ...
Y-chromosomal haplotyping of single sperm cells isolated
Y-chromosomal haplotyping of single sperm cells isolated

... sperm DNA of different donors. In this way, a mixture profile is often obtained, which can only be used for exclusion rather than identification. Another method to isolate single cells from mixtures that has been successfully used in the forensic community is laser cut microdissection (LCM) (1-5). H ...
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Vectors in gene therapy

Gene therapy utilizes the delivery of DNA into cells, which can be accomplished by several methods, summarized below. The two major classes of methods are those that use recombinant viruses (sometimes called biological nanoparticles or viral vectors) and those that use naked DNA or DNA complexes (non-viral methods).
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