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Advances in the biology of JC virus and induction
Advances in the biology of JC virus and induction

... in, and mainly confined to, the cytoplasm of JCVinfected cells, both in culture and brains of PML patients (Okada et al, 2002). Recent studies have shown that treatment of cells with neuraminidase, protease, or phospholipase blocks JCV binding. However, viral infection was specifically abrogated onl ...
Gene expression - Yale University
Gene expression - Yale University

... • Divide your table into three groups. • Each table has three Post-its and each group has a set of 12 sentence strips. • Place the Post-its on the board in order of transcription (top to bottom) ...
Biological Molecules Review KEY
Biological Molecules Review KEY

... 28. Lipids are organic compounds that are INSOLUBLE in water. In the body, they serve as LONG-term energy storage molecules. Lipids include fats, OILS, and WAXES. 29. The 3 most important classes of lipids are neutral fats, PHOSPHOLIPIDS, and. 30. Oil, fat, butter are all composed of lipid molecules ...
RNA polymerase I
RNA polymerase I

... • ~50 tRNAs in plant & animal cells, • each encoded by repeated DNA sequences • yeast: ~275, fruit flies: ~850, humans: ...
Heredity The passing of traits from parent to offspring
Heredity The passing of traits from parent to offspring

... To calculate the possible combinations of genes due to independent assortment you use the formula 2n. n = the number of chromosomes The possible # of combinations after fertilization for humans would be: 223 X 223 = over 70 trillion ...
Mendel`s First Law of Genetics (Law of Segregation)
Mendel`s First Law of Genetics (Law of Segregation)

... phenotypes in this generation. The F1 possesses the information needed to produce both parental phenotypes in the following generation. The F2 generation always produced a 3:1 ratio where the dominant trait is present three times as often as the recessive trait. Mendel coined two terms to describe t ...
2007-10_GO-resources_jblake
2007-10_GO-resources_jblake

... example here http://www.geneontology.org/GO.annotation.fields.shtml Click on ‘Annotation Files and Downloads’ [see arrow in screen shot]to look at files available for the model organism genomes. Notice that the total gene products associated values vary significantly from organism to ...
Microarray experiment guidelines
Microarray experiment guidelines

... genes, microarrays provide a large quantity of information about an organism/cell/tissue – whether it be mutational studies (monitoring the effects of gene expression by knocking out/in a particular gene), conditional (monitoring the effects on gene expression when presenting the organism/cell to a ...
Basics of Protein Expression
Basics of Protein Expression

... • Cloning in E. coli, then transfect DNA into yeast and screen for stable integration into yeast chromosome • Time required for selection of high expressing clones • Once established, rapid growth in shake flasks or ...
Model test Paper for Class 12 Biology
Model test Paper for Class 12 Biology

... iii. In rabbit species, a disease- condition called albinism develops due to recessive alleles. Let A be the allele for normal condition and “a” be the allele for albinism, such that AA and Aa represent normal phenotypes and “aa” represents the disease. Assume a large population in genetic equilibri ...
- CSHL Institutional Repository
- CSHL Institutional Repository

... Every page of ParameciumDB contains a top row of navigation tabs (Home, Search, Gbrowse, Blast, Tools, Help) and a sidebar. The sidebar on the home page (and some information pages) contains internal and external links for community news, downloads and information about specific topics such as the g ...
Supplemental Figure legends
Supplemental Figure legends

... with 5μM 5-FU for 24 hr and whole cell lysates were subjected to western blot analysis to detect p53, TRIML2 and GAPDH (loading control); Right panel: WT and p53-/- Hct116 cells were treated with 10J/M2 UV for 0, 1, 4, and 8 hr. Whole cell lysates were subjected to western blot analysis to detect p5 ...
Genes and RNA
Genes and RNA

... In order to recognize their promoters, bacterial RNA polymerase enzymes require a specialized subunit called the sigma factor (σ), which directly contacts the promoter sequence. The complex formed by the sigma subunit with the remaining polymerase core subunits constitutes the bacterial holoenzyme. ...
CalbiCyc, Metabolic Pathways at the Candida Genome Database
CalbiCyc, Metabolic Pathways at the Candida Genome Database

... Curation challenge: Steep learning curve for the curation tools. The tools are quite different, and the process is distinct, from the usual gene-centric curation we do, curators need to “switch gears” for pathway curation. Found that it was easier to make progress by making a focused “project” out o ...
PHOTOSYNTHESIS
PHOTOSYNTHESIS

... are broken and the bonds in new compounds are formed resulting in a net transfer of energy.[Clarification Statement: Emphasis is on the conceptual understanding of the inputs and outputs of the process of cellular respiration.] [Assessment Boundary: Assessment should not include identification of th ...
Research Update – June 2007 - Cure Tay
Research Update – June 2007 - Cure Tay

... has been evaluated. Some of these markers, sometimes called biomarkers, may later be used to evaluate therapeutic effect in humans. 3.5. We are evaluating the effect of the immune system on the therapeutic efficacy of gene therapy in GM2 mice. 4. Gene therapy in GM2 cats. 4.1 The first task was to i ...
View/Open
View/Open

... Transgenic plant varieties are created by taking DNA sequences from nearly any organism - ...
From Genes to Proteins
From Genes to Proteins

... When the RNA nucleotides are added during transcription, they are linked together with covalent bonds. As RNA polymerase moves down the strand, a single strand of RNA grows. Behind RNA polymerase, the two strands of DNA close up by forming hydrogen bonds between complementary bases, re-forming the D ...
Complete genome sequence of Roseophage vB_DshP
Complete genome sequence of Roseophage vB_DshP

... vRNAP gene. This vRNAP is a unique feature in N4 phages putatively conducting early transcription of infective processes. Aligning DNA polymerases of all N4 phages, which are commonly applied as one of the viral phylogenetic markers [26, 27], phage vB_DshP-R1 is shown to cluster closely with four ma ...
GeneFarm, structural and functional annotation of Arabidopsis gene
GeneFarm, structural and functional annotation of Arabidopsis gene

... genes, etc.) cannot be described using satisfactory models and this constitutes a significant limitation to the annotation pipelines (7,12). An overview of the last release (TIGR R5.0) of the Arabidopsis chromosomes shows that the associated annotation is still not optimal and, considering its pivot ...
chapter 13 meiosis and sexual life cycles
chapter 13 meiosis and sexual life cycles

Exam Questions_150216_final
Exam Questions_150216_final

... (substrates 1 and 2) or strands (substrate 3) will move slowly if still annealed to the longer DNA strand, but will move much faster if unwound and detached. The migration of these short single strands can be followed selectively by making them radioactive and examining their positions in the gel by ...
View - Journal of Pharmaceutical and Biomedical Sciences
View - Journal of Pharmaceutical and Biomedical Sciences

... frequencies in kunbi, otkar, halbi, koshti. Very few cases are also fund from teli muslim maheshwari, brhamin community. Conclusion: It is well known that sickle cell gene is widely prevalent in tribal populations. Due to the recent activities of globalization, migration of population, inter-caste m ...
Getting a grip on how DNA polymerases function
Getting a grip on how DNA polymerases function

... multiple DNA polymerases have been identified in prokaryotes and eukaryotes, including the recent discovery of several error-prone DNA polymerases2. Based on primary sequence similarity, these DNA polymerases can be categorized into families (Table 1). The most extensively studied polymerases includ ...
Chapter 1
Chapter 1

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Vectors in gene therapy

Gene therapy utilizes the delivery of DNA into cells, which can be accomplished by several methods, summarized below. The two major classes of methods are those that use recombinant viruses (sometimes called biological nanoparticles or viral vectors) and those that use naked DNA or DNA complexes (non-viral methods).
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