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... The height of a stack of letters in a logo indicates the predictive power of that stack (determined statistically). If the stack is tall, we can be more confident in predicting what base will be in that position if a new sequence is added to the logo. For example, at position 2, all 10 sequences ha ...
RBTopic3_7 Cellular Respiration - wfs
RBTopic3_7 Cellular Respiration - wfs

... 14. How many carbons does each pyruvate molecule produced by glycolysis have?_____ 15. How many pyruvate molecules are produced by glycolysis in glycolysis?__________ 16. Which type of cell respiration produces the most ATPs? ...
Two-way ANOVA - GeneSifter.Net
Two-way ANOVA - GeneSifter.Net

... 2 groups, 4 replicates each Mean, standard deviation, fold change and p-value calculated ...
Viral tropism - UK-CAB
Viral tropism - UK-CAB

... Detection of the R5/X4 phenotype ranged from about 7% in samples with CD4 counts above 300 to 46% in those with CD4 counts below 100. No exclusively X4 phenotype. Mean CD4 count for R5 samples was 307 versus 117 for R5/X4. ...
Identification of Antigenic Regions of Duck Hepatitis B Virus Core
Identification of Antigenic Regions of Duck Hepatitis B Virus Core

... plasmid encoding the entire viral genome, indicating that pCI-C plasmid expresses the entire DHBc protein of correct size. No DHBV core gene products were detected in the control cells transfected with empty pCI plasmid. Anti-DHBc humoral response elicited in ducks after DNA or protein immunization ...
Jolly Good Knowledge from the Seven Seas Monday,October 9
Jolly Good Knowledge from the Seven Seas Monday,October 9

... 1. What did Mendel experiment with to learn about genetics? Pea plants 2. A scientist who studies heredity is a geneticist. 3. Where do organisms inherit genes from?the parents 4. What are physical characteristics of an organism that are passed down from one generation to the next called? traits ...
A physical map of the genome of Hmmophilus
A physical map of the genome of Hmmophilus

... on the DNA contained in one-third of a complete plug. Restriction einzyme buffers were diffused into the agarose blocks as outlined below. Plugs or portions of plugs were washed in Eppendorf tubes with 500 1.11 vlolumesof buffer (unless stated otherwise). Fresh buffer was used for each wash. Two 30 ...
Lecture Outline
Lecture Outline

... their parents were susceptible. Variation in life cycles a. A life cycle describes the sequence of developmental events that occur as individuals grow, mature, and reproduce. b. The advent of meiosis created a distinction between haploid and diploid phases in life cycles. c. Every aspect of the life ...
01 - cloudfront.net
01 - cloudfront.net

... phenotype. He then allowed the F1 generation offspring to self-pollinate. This resulted in an F2 generation with dominant phenotypes only / both dominant and recessive phenotypes. 9. Mendel concluded that traits are inherited as “discrete units.” Today, we call these discrete units gametes / genes. ...
Chapter 19: DNA Ligases  - DNA Replication and Human
Chapter 19: DNA Ligases - DNA Replication and Human

... that contains only the latter, leaky mutation expressed in hemizygous or homozygous form. 46BR cells show inefficient joining of Okazaki fragments and anomalous gap filling during excision repair (Prigent et al. 1994). However, V(D)J joining seems to proceed normally in 46BR cells (Hsieh et al. 1993 ...
Nucleolar caspase-2: Protecting us from DNA damage
Nucleolar caspase-2: Protecting us from DNA damage

... to activated caspase-2 in the nucleolus, so it could hypothetically interact with caspase-2 or the PIDDosome activation platform. Using mass spectrometry, the authors identified an interaction between NPM1 and PIDD. Using pulldown assays, they confirmed this interaction to be damage inducible, with ...
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Data analysis approaches in high throughput screening (PDF, 3337

... a single endpoint is chosen for a screening assay. ...
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Simulating and cleaning gene expression data using

... RUV is a data-driven method that removes systematic noise from gene expression datasets. The particular version of RUV is dependent on the goal of the analysis. We have developed a method, RUVNaiveRidge, for the removal of unwanted variation that focuses on retrieving the true underlying gene-gene c ...
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Gene Flow Gene Flow Between Two Demes

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Sulfuricella denitrificans gen. nov., sp. nov., a sulfur

... successfully sequenced from genomic DNA of strain skB26T. In the phylogenetic analysis of the large subunit of form I RubisCO, encoded by the cbbL gene, the novel isolate clustered with beta- and gammaproteobacterial chemolithotrophs (Supplementary Fig. S1, available in IJSEM Online). A similar resu ...
A dioxin sensitive gene, mammalian WAPL, is implicated in
A dioxin sensitive gene, mammalian WAPL, is implicated in

... uterine cervical cancer among human tumor and normal control tissue samples examined [3]. Here, we examined mWAPL expression in normal mouse tissues by Western blot analysis and detected strong expression of mWAPL protein in the testes (Fig. 3A). Therefore, we also investigated hWAPL expression in v ...
IMMUNOLOGY ADVANCED
IMMUNOLOGY ADVANCED

... immune defects, whilst mutations of genes involved in CD40 cell signalling cause problems with the signals that are sent out by various cells - and so on.  In terms of the most common PIDs and genetic mutations, a large proportion of them have an X-linked inheritance, many have an autosomal recessi ...
MEng BIOMEDICAL ENGINEERING BEng (Hons) MEDICAL ENGINEE
MEng BIOMEDICAL ENGINEERING BEng (Hons) MEDICAL ENGINEE

... Molecular Principles of Biomedical Engineering Module No. BME4002 SECTION C: Answer ONE question only; 25 marks. ...
The past, present and future of plant breeding
The past, present and future of plant breeding

November 2013 Life Science Strand
November 2013 Life Science Strand

... What are the basics of science? The three main schools of science consist of Biology, Physical Sciences and Earth and Space Science; many of these sciences overlap in their topics. The main focus of life science is Biology, the study of living organisms (including microorganisms, plants, animals, an ...
How pupils use a model for abstract concepts in genetics
How pupils use a model for abstract concepts in genetics

... related mechanisms such as genes and DNA. The aim of the third part of the interview was to determine the interviewee’s conception of the means of genetic inheritance. If the pupil had either mentioned or heard of genes or DNA, they were asked questions such as: ‘Where do you think genes are in the ...
Optimizing bacterial expression and purifica-  Biomedical laboratory science,
Optimizing bacterial expression and purifica- Biomedical laboratory science,

... of the alleles it contains, but the sequence of the genes remains the same. Such restrictions do not however exist in the laboratory. Basically any segment of DNA can nowadays be cut out from any genome and be joined back together with any other piece of DNA. (Becker – Kleinsmith – Hardin – Bertoni ...
What is viral gastroenteritis
What is viral gastroenteritis

... of diarrhea in infants and young children under 5 years old. Adenoviruses and astroviruses cause diarrhea mostly in young children, but older children and adults can also be affected. Norwalk and Noroviruses are more likely to cause diarrhea in older children and adults. How is viral gastroenteritis ...
pdf, 1.3 MB - DNA and Natural Algorithms Group
pdf, 1.3 MB - DNA and Natural Algorithms Group

... strand displacement through competitive binding mediated by toeholds is generally used to return the machine to its initial state. The operation of these machines involves stepwise feeding and, hence, are clocked in the sense that an external operator controls when the machine advances to its next s ...
An Introduction to Genetic Analysis Chapter 20 Transposable
An Introduction to Genetic Analysis Chapter 20 Transposable

... he looked for rare wild-type Wx recombinants by staining the pollen with KI-I2 reagent, which stains Wx pollen black and wx pollen red. By counting the frequency of Wx pollen grains in each kind of heterozygote, Nelson was able to do fine-structure recombination mapping of the waxy gene. He showed t ...
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Vectors in gene therapy

Gene therapy utilizes the delivery of DNA into cells, which can be accomplished by several methods, summarized below. The two major classes of methods are those that use recombinant viruses (sometimes called biological nanoparticles or viral vectors) and those that use naked DNA or DNA complexes (non-viral methods).
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