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... ddGTP is added at ~1/100th the amount of dGTP. To achieve termination at each type of base, four separate reactions are run in parallel using the sequencing template (right, below). Each reaction is spiked with one of the four ddNTPs. ...
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DNA Replication - Madison County Schools

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Vectors in gene therapy

Gene therapy utilizes the delivery of DNA into cells, which can be accomplished by several methods, summarized below. The two major classes of methods are those that use recombinant viruses (sometimes called biological nanoparticles or viral vectors) and those that use naked DNA or DNA complexes (non-viral methods).
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