Nucleotide Sequence of the Osmoregulatory proU Operon of
... amino acids long (Mr 37,619). There is an 8-nucleotide overlap between the end of proV and the start of proW, suggesting that there may be translational coupling in the expression of the two genes; a ribosome-binding site is also present upstream of the proW initiation site (Fig. 2), a feature that ...
... amino acids long (Mr 37,619). There is an 8-nucleotide overlap between the end of proV and the start of proW, suggesting that there may be translational coupling in the expression of the two genes; a ribosome-binding site is also present upstream of the proW initiation site (Fig. 2), a feature that ...
A tale of two functions: enzymatic activity and
... electropositive surface potential, whereas the rest of the protein has a net electronegative surface potential (6). Zinc-finger domains are commonly associated with nucleic acid-binding proteins and previous studies have shown that CT does bind DNA, albeit non-specifically (7). Notably, DNA binding in ...
... electropositive surface potential, whereas the rest of the protein has a net electronegative surface potential (6). Zinc-finger domains are commonly associated with nucleic acid-binding proteins and previous studies have shown that CT does bind DNA, albeit non-specifically (7). Notably, DNA binding in ...
Hybrid Plasmids Containing the Pyruvate
... broth [L broth supplemented with 0.1% (w/v) glucose] for the growth of ace and lpd mutants. This was supplemented with thymine (50 pg ml-l) and antibiotics (pg ml-*): streptomycin, 200; ampicillin, 50; tetracycline, 20; and chloramphenicol, 25 or 100, as required. Phage assays were made with BBL med ...
... broth [L broth supplemented with 0.1% (w/v) glucose] for the growth of ace and lpd mutants. This was supplemented with thymine (50 pg ml-l) and antibiotics (pg ml-*): streptomycin, 200; ampicillin, 50; tetracycline, 20; and chloramphenicol, 25 or 100, as required. Phage assays were made with BBL med ...
Abstract
... previous step was then repeated. Both of these steps were done in order to extract the majority of the proteins out of the sample. We then added 150 microliters of 5M NaCl. This helped to remove the carbohydrates from the solution. Isopropyl alcohol was then added to the tube and the tube was invert ...
... previous step was then repeated. Both of these steps were done in order to extract the majority of the proteins out of the sample. We then added 150 microliters of 5M NaCl. This helped to remove the carbohydrates from the solution. Isopropyl alcohol was then added to the tube and the tube was invert ...
RECOMBINANT DNA TECHNOLOGY AND BIOTECHNOLOGY
... addition to this particular methylase, individual bacterial strains also contain accompanying specific endonuclease activity. In late 1960s, Stewart Linn and Werner Arber isolated two types of enzymes responsible for this observed phage restriction phenomenon. Later on, in 1978, Smith, Nathans and ...
... addition to this particular methylase, individual bacterial strains also contain accompanying specific endonuclease activity. In late 1960s, Stewart Linn and Werner Arber isolated two types of enzymes responsible for this observed phage restriction phenomenon. Later on, in 1978, Smith, Nathans and ...
Applied Environmnetal Microbiology
... growth by phase-contrast microscopic observations. The CryV1 and CryV465 proteins were partially purified from E. coli recombinant strains as described in Materials and Methods, and SDS-PAGE analysis followed by densitometric scanning analysis showed that about 48% of the total protein was the 81-kD ...
... growth by phase-contrast microscopic observations. The CryV1 and CryV465 proteins were partially purified from E. coli recombinant strains as described in Materials and Methods, and SDS-PAGE analysis followed by densitometric scanning analysis showed that about 48% of the total protein was the 81-kD ...
NucleoSpin 96 Flash Plasmid and Large-Construct DNA
... soluble proteins and other cell constituents. Afterwards, all precipitates are removed by filtration of the lysate through the NucleoSpin® 96 Flash Filter Plate under vacuum or in a suitable centrifuge for microtiter plates. Plasmid DNA is precipitated by addition of isopropanol to the filtrate and ...
... soluble proteins and other cell constituents. Afterwards, all precipitates are removed by filtration of the lysate through the NucleoSpin® 96 Flash Filter Plate under vacuum or in a suitable centrifuge for microtiter plates. Plasmid DNA is precipitated by addition of isopropanol to the filtrate and ...
Vital Genes in the Heterochromatin of
... Joint efforts are now required to complete the molecular characterization of the heterochromatic genome of D. melanogaster and to extend the work to other Drosophila species. This may allow us to characterize new heterochromatic genes and to learn how they have evolved. An important goal is also to ...
... Joint efforts are now required to complete the molecular characterization of the heterochromatic genome of D. melanogaster and to extend the work to other Drosophila species. This may allow us to characterize new heterochromatic genes and to learn how they have evolved. An important goal is also to ...
AnnotatorsInterface-GUS
... Provide alignment of features on genomic sequence – could potentially display any feature type currently stored in GUS3.0 – features can be selected and used to generate “curated” features – similar to display and functionality in Apollo ...
... Provide alignment of features on genomic sequence – could potentially display any feature type currently stored in GUS3.0 – features can be selected and used to generate “curated” features – similar to display and functionality in Apollo ...
PCR based detection and quantification of GMO potatoes, utilization
... The need to monitor and verify the presence and the amount of GMOs in agricultural crops and products has generated a demand for analytical methods capable of detecting, identifying and quantifying either the DNA introduced or the protein(s) expressed in transgenic plants, because these components a ...
... The need to monitor and verify the presence and the amount of GMOs in agricultural crops and products has generated a demand for analytical methods capable of detecting, identifying and quantifying either the DNA introduced or the protein(s) expressed in transgenic plants, because these components a ...
Apolipoprotein A5, a Newly Identified Gene That Affects Plasma
... Genomics and the Identification of APOA5 Recent accomplishments by the Human Genome Project have facilitated genome-wide strategies to uncover functional regions of the mammalian genome. With the increasing availability of genomic sequences from multiple species, comparative genomic approaches have ...
... Genomics and the Identification of APOA5 Recent accomplishments by the Human Genome Project have facilitated genome-wide strategies to uncover functional regions of the mammalian genome. With the increasing availability of genomic sequences from multiple species, comparative genomic approaches have ...
Evaluation of existing motif detection tools on their
... GOAL: get insight in molecular mechanism underlying anti-proliferative effect of vitD3 - Human and mouse cell lines treated with vitD3 versus no vitD3 (Control) - Measured the expression of all genes in the human and mouse cells using microarrays for both conditions over different time points - Sele ...
... GOAL: get insight in molecular mechanism underlying anti-proliferative effect of vitD3 - Human and mouse cell lines treated with vitD3 versus no vitD3 (Control) - Measured the expression of all genes in the human and mouse cells using microarrays for both conditions over different time points - Sele ...
Apolipoprotein A5, a Newly Identified Gene That Affects Plasma
... Genomics and the Identification of APOA5 Recent accomplishments by the Human Genome Project have facilitated genome-wide strategies to uncover functional regions of the mammalian genome. With the increasing availability of genomic sequences from multiple species, comparative genomic approaches have ...
... Genomics and the Identification of APOA5 Recent accomplishments by the Human Genome Project have facilitated genome-wide strategies to uncover functional regions of the mammalian genome. With the increasing availability of genomic sequences from multiple species, comparative genomic approaches have ...
Cot-1 banding of human chromosomes using fluorescence
... suggested that repetitive sequences other than Alu or KpnI are apparently contained in the human Cot-1 D N A preparation and caused the positive bands on the heterochromatin regions. The banding pattern obtained from Cot-1 DNA hybridization to metaphase chromosomes is reproducible and may be used as ...
... suggested that repetitive sequences other than Alu or KpnI are apparently contained in the human Cot-1 D N A preparation and caused the positive bands on the heterochromatin regions. The banding pattern obtained from Cot-1 DNA hybridization to metaphase chromosomes is reproducible and may be used as ...
e Study of RNA Polymerase Pausing by Optical Traps
... duration. The cell implements different pausing mechanisms based on the conditions and nutrient requirements in the cell. For example, in Escherichia coli, pausing might couple transcription and translation by synchronizing the interaction between RNA synthesis and ribosome movement in the coding po ...
... duration. The cell implements different pausing mechanisms based on the conditions and nutrient requirements in the cell. For example, in Escherichia coli, pausing might couple transcription and translation by synchronizing the interaction between RNA synthesis and ribosome movement in the coding po ...
Full text in pdf - International Microbiology
... Summary. The non-phosphorylating glyceraldehyde 3-phosphate dehydrogenase (GAPDHN, NADP+-specific, EC 1.2.1.9) is present in green eukaryotes and some Streptococcus strains. The present report describes the results of activity and immunoblot analyses, which were used to generate the first survey of ...
... Summary. The non-phosphorylating glyceraldehyde 3-phosphate dehydrogenase (GAPDHN, NADP+-specific, EC 1.2.1.9) is present in green eukaryotes and some Streptococcus strains. The present report describes the results of activity and immunoblot analyses, which were used to generate the first survey of ...
NIH Guidelines for Research Involving rDNA Molecules (NIH
... History of the Guidelines • The NIH Guidelines were implemented in response to public and scientific concern over the emerging science of rDNA technologies in the early 1970’s. • By 1976, NIH had published the first set of guidelines which have been amended over time to allow for greater public acc ...
... History of the Guidelines • The NIH Guidelines were implemented in response to public and scientific concern over the emerging science of rDNA technologies in the early 1970’s. • By 1976, NIH had published the first set of guidelines which have been amended over time to allow for greater public acc ...
tutorial5_12
... • TOMTOM - Find the protein who binds the DNA motifs. • GOMO - Find putative target genes (DNA) of motifs and analyze their associated annotation terms. • PROSITE - Search for them in annotated protein sequence databases. ...
... • TOMTOM - Find the protein who binds the DNA motifs. • GOMO - Find putative target genes (DNA) of motifs and analyze their associated annotation terms. • PROSITE - Search for them in annotated protein sequence databases. ...
Transcription factories are nuclear subcompartments that remain in
... longer associated with RNAPII foci. These results show that initiation inhibition results in a significant dissociation of previously active Hbb alleles from transcription factories (P = 0.000033). In the case of Eraf, 43% of alleles are associated with factories in untreated cells (Fig. 3A,B), refl ...
... longer associated with RNAPII foci. These results show that initiation inhibition results in a significant dissociation of previously active Hbb alleles from transcription factories (P = 0.000033). In the case of Eraf, 43% of alleles are associated with factories in untreated cells (Fig. 3A,B), refl ...
Genetic Art - Northwestern University
... rules for combining constants and functions to form expressions: {All operators take any 2 constants, except /, which can not take 0 for it’s second argument} ...
... rules for combining constants and functions to form expressions: {All operators take any 2 constants, except /, which can not take 0 for it’s second argument} ...