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Genetics notes
Genetics notes

... • Mutations: changes that occur in a gene or chromosome. Mutations can occur for a variety of reasons. For example, when chromosomes (genes) separate and reform during meiosis a segment of one gene will sometimes switch places with a segment on the other DNA strand. This is called crossing over and ...
Lectre 10
Lectre 10

... • DNA ligase is a enzyme that can link together DNA strands that have double-strand breaks (a break in both complementary strands of DNA). – Naturally DNA ligase has applications in both DNA replication and DNA repair . – Needs ATP ...
Targeted Fluorescent Reporters: Additional slides
Targeted Fluorescent Reporters: Additional slides

... How does a proto-oncogene that is functioning normally and in a healthy fashion become an oncogene? What are the genetic changes? a) A gene can move to a new location and become under the control of a new promoter that is more active and thus more of the gene’s product is produced which stimulates t ...
DNA
DNA

... heredity material control the making of proteins? ...
Lecture 15 - Psychology
Lecture 15 - Psychology

... If marker and trait gene are far away from one another, independent assortment occurs This pedigree demonstrates a random association with the A allele and the disorder, which indicates that A and D are not linked ...
e) Describe the structure of a bacterial chromosome including the
e) Describe the structure of a bacterial chromosome including the

... 2. donor cell has F-plasmid / with F-factor genes for pilli production 3. donor cell = F+ cell form cytoplasmic bridge to recipient cell 4. F-plasmid DNA separates into 2 single strands 5. one strand is transferred across the bridge 6. both act as templates for synthesis of daughter strands 7. plasm ...
Molecular diagnosis and inborn errors of metabolism
Molecular diagnosis and inborn errors of metabolism

... pendent on the nature and level of the accumulated or deficient metabolites than it is on the specific gene mutation. The next great frontier in inborn errors, understanding and interrupting the pathogenic process, may be completely dependent on knowledge of the metabolites, although their effect on ...
The relationship between genes and traits is often complex
The relationship between genes and traits is often complex

... and traits is often complex Complexities include: • Complex relationships between alleles • Multiple genes controlling one trait • One gene controlling multiple traits • Environmental effects ...
Plasmid Isolation
Plasmid Isolation

... • Each restriction enzyme cuts at different sites • The cut can leave either blunt or sticky ends • If the plasmid and gene to be inserted into the plasmid are cut with the same restriction enzyme, the sticky ends have overlapping base pairs and can anneal with DNA ligase • But, the cut ends can ann ...
this document - Institute for Agriculture and Trade Policy
this document - Institute for Agriculture and Trade Policy

... promoter has been shown to be extra unstable in GM crops. And horizontal transfer of transgenic DNA has been demonstrated both in the laboratory and in the field. Proponents of GM technology had attempted to refute our warnings about the CaMV 35S promoter [8]. But they have failed to counter our poi ...
Informed Consent for TPMT Genetic Tests
Informed Consent for TPMT Genetic Tests

06BIO201 Exam 3 KEY
06BIO201 Exam 3 KEY

... a. attach an enhancer and a basal transcription factor binding site (promoter) from a gene expressed in spider back cells onto the wing gene and then introducing this artificially engineered gene into a culture of spider back cells b. attach an enhancer and a basal transcription factor binding site ...
DNA - The Double Helix (Article)
DNA - The Double Helix (Article)

... So, now, we know the nucleus controls the cell's activities through the chemical DNA, but how? It is the sequence of bases that determine which protein is to be made. The sequence is like a code that we can now interpret. The sequence determines which proteins are made, and the proteins determine wh ...
Biology 207 Workshop 9
Biology 207 Workshop 9

... fragments appear darker or more intense than bands containing smaller sized DNA fragments. In the autoradiogram, all the bands occur with equal intensity. Assuming that all the bands have the same number of DNA molecules can you explain this result? Ethidum bromide intercalates between the paired nu ...
Mutations
Mutations

... Chromosomal Mutations • Nondisjunction = failure of homologous chromosomes to separate during meiosis resulting in gametes (egg or sperm) with too few or too many chromosomes • Animation: ...
The Structure
The Structure

... The zinc finger is a common structural motif in many protein DNA binding domains. It is found in roughly 3%(!) of all proteins and performs a diverse range of functions not limited to enhancing or inhibiting one specific gene. They are one of several domains that evolved to solve the problem of sequ ...
MUTATIONS
MUTATIONS

... that is not immediately and properly repaired, or any inherited change in the sequence of DNA.  Mutations that occur in the body cells cause cell death or cancer, and are not passed on to the next generation.  Mutations are usually recessive and are inherited in a Mendelian way. ...
Chapter 12 DNA & RNA
Chapter 12 DNA & RNA

... Watson & Crick • Francis Crick, a British physicist & James Watson, an American biologist • trying to understand the structure of DNA – by building three-dimensional models of the molecule ...
DNA RNA
DNA RNA

... promoter on DNA, site RNA polymerase binds to on DNA to begin transcription). - A mRNA is transcribed (built) using unwound DNA’s code as a template. - RNA nucleotides find their compliment. - RNA Polymerase is the enzyme that brings in the nucleotides ...
Topic 6: Genetics Page 1
Topic 6: Genetics Page 1

Gene Expression - M.Tevfik DORAK`s
Gene Expression - M.Tevfik DORAK`s

... Expression of the human b-globin gene. Exons 1 and 3 each contain noncoding sequences (shaded bars) at their extremities, which are transcribed and are present at the 5’ and 3’ ends of the b-globin mRNA, but are not translated to specify polypeptide synthesis. Such 5’ and 3’ untranslated regions ( ...
Livenv_genetics - OurTeachersPage.com
Livenv_genetics - OurTeachersPage.com

... Key Findings of the Project… • 1. There are approx. 30,000 genes in human beings, the same range as in mice and twice that of roundworms. Understanding how these genes express themselves will provide clues to how diseases are caused. • 2. All human races are 99.99 % alike, so racial differences are ...
Assay for Methylation of genes
Assay for Methylation of genes

... There are two major genetic pathways leading to colorectal cancer. About 80% of colon cancers are caused by chromosomal instability, with gross changes in chromosome number and integrity. These are microsatellite stable (MSS). Microsatellite instability (MSI) is observed in about 1015% of sporadic c ...
DNA and RNA
DNA and RNA

... Based on his results, Griffith hypothesized that when the harmless and heat-killed bacteria where mixed, some factor was exchanged between them, making the live harmless bacteria deadly. Transformation – process in which one strain of bacteria is changed by the gene(s) of another bacteria ...
DNA Microarrays
DNA Microarrays

... answer many basic questions ...
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Helitron (biology)

A helitron is a transposon found in eukaryotes that is thought to replicate by a so-called ""rolling-circle"" mechanism. This category of transposons was discovered by Vladimir Kapitonov and Jerzy Jurka in 2001. The rolling-circle process begins with a break being made at the terminus of a single strand of the helitron DNA. Transposase then sits at this break and at another break where the helitron targets as a migration site. The strand is then displaced from its original location at the site of the break and attached to the target break, forming a circlular heteroduplex. This heteroduplex is then resolved into a flat piece of DNA via replication. During the rolling-circle process, DNA can be replicated beyond the initial helitron sequence, resulting in the flanking regions of DNA being ""captured"" by the helitron as it moves to a new location.
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