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Basic molecular genetics for epidemiologists
Basic molecular genetics for epidemiologists

... DNA that does not seem to have any function. In fact, the human genome is riddled with sequences that derive from non-pathogenic viruses that inserted their DNA into the human genome, and that have been inadvertently copied ever since. Mitochondrial DNA (mtDNA) Small circular DNA molecule contained ...
Practice test 2
Practice test 2

... DNA would be classified as a _____. a. clone c. plasmid b. DNA fingerprint d. transgenic organism 8. In 1974, Stanley Cohen and Herbert Boyer inserted a gene from an African clawed frog into a bacterium. The bacterium produced the protein coded for by the inserted frog gene. This insertion of a smal ...
Chapter 16 PPT
Chapter 16 PPT

... • DNA can be damaged by chemicals, radioactive emissions, X-rays, UV light, and certain molecules (in cigarette smoke for example) • In nucleotide excision repair, a nuclease cuts out and replaces damaged stretches of DNA Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummin ...
Molecular Genetics
Molecular Genetics

... A gene is a segment of DNA that specifies the sequence of amino acids in a polypeptide  Suggests that genetic mutations cause changes in the primary structure of a protein ...
Genetics(Semester(One,(Year(Two!
Genetics(Semester(One,(Year(Two!

... homozygous.$This$is$due$to$gene$product$interactions.$Heterozygous$means$two$different$gene$products$may$affect$ each$other,$homozygous$is$only$one$gene$product$(may$give$different$phenotype)$ At$the$DNA$sequence$level$all$alleles$are$co\dominant.$At$a$molecular$level,$protein$production$sequence.$ ...
unit iv - dna & cell division
unit iv - dna & cell division

... molecules  Result of replication  Term is only used when identical DNAs are physically attached; described as one chromosome made up of two sister chromatids  Centromere – Site where sister chromatids are most closely attached ...
video slide
video slide

... EXPERIMENT Researchers had two mutant strains, one that could make arginine but not tryptophan (arg+ trp–) and one that could make tryptophan but not arginine (arg trp+). Each mutant strain and a mixture of both strains were grown in a liquid medium containing all the required amino acids. Samples ...
DNA Extraction from Fruit - DNALC Lab Center
DNA Extraction from Fruit - DNALC Lab Center

... Have another student read step 5 from the procedure, while the rest of the class listens. Instruct students to draw a diagram of what will happen the precipitation with ethanol is completed in step 5. Make sure students understand that ethanol is a type of alcohol, similar to rubbing alcohol. Becaus ...
enstour_
enstour_

... The predicted genes are stored in the database so that they can be retrieved later. ...
DNA Technology Notes
DNA Technology Notes

... - heat filter paper to denature DNA - wash filter paper with radioactive probe which will only attach to gene of interest ...
Blueprint of Life by Ahmad Shah Idil
Blueprint of Life by Ahmad Shah Idil

... accompanied by selection, allow change over many generations:  Outline the impact on the evolution of plants and animals of:  Changes in the physical conditions in the environment:  Changes in the chemical condition in the environment: ...
here - PHI-base
here - PHI-base

... Reduced virulence – the transgenic strain still causes some disease formation but this is less than the wild-type strain (ie. a quantitative effect). Synonymous with the term reduced aggressiveness. Unaffected pathogenicity - the transgenic strain which expresses no or reduced levels of a specific g ...
PDF995, Job 12
PDF995, Job 12

... During the past five years, random cloning of microbial DNA directly isolated from environmental materials and subsequent screening of expression libraries for the presence of a desired enzyme activity has become a useful tool for the discovery of novel biocatalysts. The collective genomes of microb ...
as a PDF
as a PDF

... The PvuII restriction endonuclease has been converted from its natural homodimeric form into a single polypeptide chain by tandemly linking the two subunits through a short peptide linker. The arrangement of the single-chain PvuII (sc PvuII) is (2-157)-GlySerGlyGly-(2-157), where (2157) represents t ...
Transcription factors - introduction
Transcription factors - introduction

... requirement is to form the initiation complex • although other forms of regulation are important, the majority of regulatory events occur at the initiation of transcription – genes under common control share response elements (aka cis-cting elements, enhancers) • these sequences are presumed to be r ...
Individual eukaryotic genomes
Individual eukaryotic genomes

... sequenced. Mouse diverged from human 75 MYA. Distinguishing features: only 300 of 30,000 annotated genes have no human orthologs Genome size: 2.5 Gb (euchromatic portion)(cf. 2.9 Gb human) Chromosomes: 6 Genes: about 30,000 Website: http://www.informatics.jax.org --Dozens of mouse-specific expansion ...
Vocabulary: Did you know?
Vocabulary: Did you know?

... Genotype-­‐  Your  genotype  is  the  composition  of  alleles  you  have  for  a  particular   gene  or  genes.  Remember  than  many  genes  come  in  two  or  more  different  “flavors”   or  alleles—one  version  (or  allele)  may ...
Automation of Genomic DNA Isolation with Nucleic
Automation of Genomic DNA Isolation with Nucleic

... panel of genes for which amplification was carried with specific primers. The automated process was observed to be significantly efficient as no DNA was detected in the wash and extra elution steps except the actual elution step. The isolated DNA yield was 4.9µg/500µL of human saliva with an OD260/2 ...
AP & Regents Biology
AP & Regents Biology

...  Place an order at your local DNA lab! AP Biology ...
GPS standards
GPS standards

... Counterfeiting Ransom notes manner of death cause and mechanism of death time of death characteristics of bones what bones can tell us skeletal trauma analysis ...
HO DNA Necklace Lab Report
HO DNA Necklace Lab Report

... extend like a ribbon through the entire ethanol layer. DNA yields will naturally vary within the class and not all DNA samples will extend through the entire ethanol layer. 9. Watch closely as wispy strands of translucent DNA begin to clump together where the ethanol layer meets the cell lysate laye ...
DNA Replication
DNA Replication

... • Environmental factors such as hazardous chemicals or radiation are one source of genetic mistakes that do occur. • Can cause uncomplimentary bases to join. • Permanent damage is prevented by enzyme that act as proof readers. They run along the strands of DNA looking for mismatched pairs. It snips ...
Lesson Objectives: You must be comfortable doing these items:
Lesson Objectives: You must be comfortable doing these items:

... Occasionally, a mutation may make a protein even better than it was before. Or the protein might help the organism adapt to a new environment. These mutations are considered beneficial. An example is a mutation that helps bacteria resist antibiotics. Bacteria with the mutation increase in numbers, s ...
RegulomeDB - Ritchie Lab
RegulomeDB - Ritchie Lab

... • So there is a vast region to explore – the affect of genetic variation on transcription • What are useful sources for identifying regulatory elements? • The GWAS example • You have performed a GWAS • There are 10 SNPs of interest passing your p-value cutoff • Looks like 3 of the SNPs are within pr ...
Regulating Gene Expression
Regulating Gene Expression

... the longer there is for the necessary mutations to accumulate  Viruses also play a role in the development of some cancers  Retroviruses have oncogenes that can be donated to the host cell  The viral DNA may also be inserted in such a way that it disrupts a tumor-supressing gene. ...
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Helitron (biology)

A helitron is a transposon found in eukaryotes that is thought to replicate by a so-called ""rolling-circle"" mechanism. This category of transposons was discovered by Vladimir Kapitonov and Jerzy Jurka in 2001. The rolling-circle process begins with a break being made at the terminus of a single strand of the helitron DNA. Transposase then sits at this break and at another break where the helitron targets as a migration site. The strand is then displaced from its original location at the site of the break and attached to the target break, forming a circlular heteroduplex. This heteroduplex is then resolved into a flat piece of DNA via replication. During the rolling-circle process, DNA can be replicated beyond the initial helitron sequence, resulting in the flanking regions of DNA being ""captured"" by the helitron as it moves to a new location.
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