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Biomolecules - Greater Clark County Schools
Biomolecules - Greater Clark County Schools

... PROTEINS  Proteins are found everywhere.  Proteins make up our hair, skin, nails, organs and ...
Precise insertion and guided editing of higher plant
Precise insertion and guided editing of higher plant

Next-Generation DNA Sequencing Methods
Next-Generation DNA Sequencing Methods

... The fragment libraries are obtained by annealing platform-specific linkers to blunt-ended fragments generated directly from a genome or DNA source of interest. Because the presence of adapter sequences means that the molecules then can be selectively amplified by PCR, no bacterial cloning step is requ ...
p53 Sequencing for Li-Fraumeni Syndrome
p53 Sequencing for Li-Fraumeni Syndrome

... mutations; explanations for the lack of identifiable p53 mutations in 30-50% of classic LFS families have yet to be elucidated. It is also important to note that germline p53 mutations have also been identified in LFS-L families and in a small percentage of patients with cancers not typically associ ...
center - University of California, Santa Cruz
center - University of California, Santa Cruz

... • knownToGenePix - makes table in mm5 (or other) genome database to connect known genes to genePix Ids. ...
“Indeed, the Homeobox has been called the `Rosetta Stone` of
“Indeed, the Homeobox has been called the `Rosetta Stone` of

... HoxB1 ...
Understanding Genetics: Punnett Squares
Understanding Genetics: Punnett Squares

... 1. Have students change the genotypes of one the parent rabbits to all dominant genes (SSEE) and construct and complete a new Punnett Square matrix. 2. Have students extend the Punnett Square to include all of the possible combinations for 2nd generation “grand-rabbits” from the parent pair from Ext ...
module three
module three

... Most horse genes have far more DNA in them than is actually needed to code for the amino acids in their polypeptide products. Within the coding DNA, known as exons, are stretches of non-coding DNA, called introns. Many genes are composed mostly of introns. When transcription occurs all of the DNA ba ...
MICRO-MANIPULATION OF CHICKEN CHROM OSOMES AND
MICRO-MANIPULATION OF CHICKEN CHROM OSOMES AND

... A chromosome 1 library of small inserts has been generated and is being used for identification of clones containing microsatellite sequences. The chromosome cocktail has been used, both as a painting probe and as a probe for identification of chromosome 1 cqspaid clones. This latter use of the chro ...
Mutations
Mutations

... DNA polymerase proofreads the polymerisation step before proceeding to the next one Incorrect bases are removed by the 3’5’ exonuclease Some E. coli mutants with abnormally high mutation rates have an altered DNA polymerase II with lowered 3’5’ exonuclease activity ...
Gene Section NEIL1 (nei endonuclease VIII-like 1 (E. coli))
Gene Section NEIL1 (nei endonuclease VIII-like 1 (E. coli))

... deoxyribo-5'-phosphate (dRP) and excised by a dRP lyase (dRPase) activity of DNA polymerase beta. Since NEIL1 also has dRPase activity, NEIL1 has a role as a backup dRPase in mammalian cells. (5) NEIL1 has a repair activity for oxidized bases in single-strand DNA and bubble DNA, suggesting a possibi ...
Pharmacogenomics Module Presentation
Pharmacogenomics Module Presentation

... related to the phenotype of PTC taste ...
Molecular Genetics
Molecular Genetics

... You all know that you start out with one cell and therefore one set of genes, right? You also know that those genes contain instructions for a cell’s structure and function, right? So then how can your liver cells and your brain cells contain the same genes but be so different? In this unit you will ...
Identification of a Class of Chromatin Boundary Elements
Identification of a Class of Chromatin Boundary Elements

... Boundary elements are thought to define the ends of functionally independent domains of genetic activity. An assay for boundary activity based on this concept measures the ability to insulate a bracketed, chromosomally integrated reporter gene from position effects. Despite their presumed importance ...
PowerPoint - Oregon State University
PowerPoint - Oregon State University

... an ndk mutant results more directly from altered dNTP pools or from loss of a DNA repair activity associated with the enzyme. After successful transformation, the mutation rates will be measured. An assay for dNTP pool levels will be performed using a scintillation counter. NDP kinase enzyme activit ...
Comparison of Genomes using High-Performance - FACOM
Comparison of Genomes using High-Performance - FACOM

... The comparison of genomes has the potential of revealing evolutionary relationships of seemingly different organisms. Xanthomonas for example is a group of bacteria, where Xanthomonas axonopodis pv. citri or Xac causes citrus canker in citrus trees, while Xanthomonas campestris pv. campestris or Xcc ...
Mutation Nomenclature Extensions and Suggestions to Describe
Mutation Nomenclature Extensions and Suggestions to Describe

... In diseases such as Duchenne Muscular Dystrophy (DMD), many mutations are found which delete (sets of) whole exons, detected on Southern blot using cDNA probes or using exon specific PCR-tests. Current rules for mutation description do not cover these, with the consequence that everybody uses their ...
the genetic material
the genetic material

... structure; one of the two general categories of nitrogenous bases found in DNA& RNA; thymine, cytosine or uracil  Base-Pairing Rules: the rules stating that cytosine pairs w/ guanine & adenine pairs w/ thymine in DNA & adenine pairs w/ uracil in RNA ...
Molecular Biology Fundamentals
Molecular Biology Fundamentals

... nucleotides adenine, cytosine, guanine, and thymine. Since a repeating polymer consisting of four subunits could not encode information, it was widely held that DNA provided only a structural role in chromosomes and that genetic information was stored in protein. ...
After giving a short brief report about importance of DNA molecules
After giving a short brief report about importance of DNA molecules

... Braun et al. [11] In this approach sticky ends of DNA (single-stranded ‘‘overhang’’ regions) are hybridized to short surface-bound oligomers. After this novel work some other similar experimental work have been done by Zhang et al. and Hartzell et al. [12,23] Similarly, DNA modified with thiol (SH) ...
RNA
RNA

... The mRNA then enters the cytoplasm and attaches to a ribosome. Translation begins at AUG, the start codon. Each transfer RNA has an anticodon whose bases are complementary to a codon on the mRNA strand. The ribosome positions the start codon to attract its anticodon, which is part of the tRNA that b ...
CHAPTER 1 MOLECULAR BIOLOGY FOR THE PRACTICAL BIOINFORMATICIAN
CHAPTER 1 MOLECULAR BIOLOGY FOR THE PRACTICAL BIOINFORMATICIAN

... instructions—also known as the “genome”—contains all the instructions for everything our cells do from conception until death. These genetic instructions on the DNA are scanned by the ribosome molecules in the cells. Just like Turing’s read/write heads, the ribosome molecules methodically decipher t ...
resistance. Section 7.5 Questions, page 345 1. (a) A mutation is a
resistance. Section 7.5 Questions, page 345 1. (a) A mutation is a

... (UAA) at the start of the mRNA transcript and the peptide will not be synthesized. This is a nonsense mutation. (c) The removal of the thymine in position 10 is a frame shift mutation. The resulting DNA sequence, 3'-ATA CCC GCC TTT CGT ACT TCC TAG-5', would code for the peptide with altered amino ac ...
Cell Division
Cell Division

Faith and the Human Genome
Faith and the Human Genome

... about 30,000 for the number of human genes. Considering that we’ve been talking about 100,000 genes for the last fifteen years (that’s what most of the textbooks still say), this was a bit of a shock. In fact, some people took it quite personally. I think they were particularly distressed because th ...
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Helitron (biology)

A helitron is a transposon found in eukaryotes that is thought to replicate by a so-called ""rolling-circle"" mechanism. This category of transposons was discovered by Vladimir Kapitonov and Jerzy Jurka in 2001. The rolling-circle process begins with a break being made at the terminus of a single strand of the helitron DNA. Transposase then sits at this break and at another break where the helitron targets as a migration site. The strand is then displaced from its original location at the site of the break and attached to the target break, forming a circlular heteroduplex. This heteroduplex is then resolved into a flat piece of DNA via replication. During the rolling-circle process, DNA can be replicated beyond the initial helitron sequence, resulting in the flanking regions of DNA being ""captured"" by the helitron as it moves to a new location.
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