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Beads on a string Bowater Biochem Soc Trans 2012
Beads on a string Bowater Biochem Soc Trans 2012

... unit of chromatin, the nucleosome, and have established roles for covalent modifications of histones as signals for gene regulation, epigenetic effects, and they continue to provide us with understanding of other biological processes as shown most recently by studies of the role of chromatin in the ...
Sheared DNA fragment sizing: comparison of techniques
Sheared DNA fragment sizing: comparison of techniques

... For some experiments it is necessary to use long (>1000 base pair) fragments of DNA. Fragments of this size have been used to determine the extent of inter2 3 4 sperslon of repeated and nonrepeated sequences of DNA in eucaryotes ' ' In general, however, on kinetic and other grounds it is advantageou ...
Human Pif1 helicase is a G-quadruplex DNA
Human Pif1 helicase is a G-quadruplex DNA

... by full-length hPif1 and the core helicase domain hPifHD. With ss/dsDNA substrates, such as PST55, the unwinding activity of full-length hPif1 increased steeply from little or no unwinding at 1 nM to a peak at 2 nM and decreasing thereafter. The reason why this activity profile is observed is unclea ...
Repeat mediated gene duplication in the Drosophila
Repeat mediated gene duplication in the Drosophila

... was added, and this longer sequence was used to identify the endpoints (via BLAST). This process was repeated until the alignment no longer terminated at the end of the partition, and the duplication endpoint was identified. Protein coding sequences were inferred using the initial published annotatio ...
Applied and Environmental Microbiology
Applied and Environmental Microbiology

... Acidobacterium phylum and actinomycetes showed up in the PCR products when isolated DNA was used as the template, whereas sequences obtained for nifH and for the denitrification genes were closely related to those of the proteobacteria. Although in such an experimental approach one has to cope with ...
Document
Document

... 3. Do statistical analysis to determine which one SSR is the most likely to be linked to the trait locus, given the pedigree data we have. 4. Narrow in on the genes present in the genome near to that SSR, and find the right one out of these candidates ...
Computational Biology
Computational Biology

... Drawbacks of breakpoint analysis: costly + ambiguous Let us consider a simple example: Suppose that the genomes G1, G2, and G3, evolved from the ancestral genome A = 1 2 3 4 5 6 by one reversal each such that G1 = 1 2 -4 -3 5 6 G2 = 1 -4 -3 -2 5 6 G3 = 1 2 3 4 -5 6 Searching for the breakpoint medi ...
Honor Genetics DNA structure and replication
Honor Genetics DNA structure and replication

... After reaching the next RNA primer (not shown), DNA pol III falls off. Okazaki fragment ...
Chapter 7  - McGraw Hill Higher Education
Chapter 7 - McGraw Hill Higher Education

... information that each cell needs to produce proteins. These instructions make life possible. In fact, before a cell divides, it first makes an exact replica of its DNA. This process, described in chapter 8, copies the precious information that will enable the next generation of cells to live. Given ...
Uniprot - European Bioinformatics Institute
Uniprot - European Bioinformatics Institute

... • Ensembl and UniProt collaboration to cover the gaps in gene predictions in UniProtKB (one sequence for each protein coding transcript in Ensembl) • Ensembl high quality gene/transcript models (quality checks remove gene models with erroneous structures or supported by dubious evidence – e.g. cDNA ...
slides pdf - Auburn University
slides pdf - Auburn University

... of three bases that instruct for the addition of a particular amino acid (or a stop) ...
Biol 1020: Genes and how they work
Biol 1020: Genes and how they work

... of three bases that instruct for the addition of a particular amino acid (or a stop) ...
Simple Sequence Repeats as Advantageous Mutators
Simple Sequence Repeats as Advantageous Mutators

... tract in the MC1R gene for a melanocortin receptor expressed in pig melanocytes. Frameshifting caused by germ-line addition of an extra C in this SSR leads to loss of pigmentation, while somatic cell reversions to the original tract length occur at relatively high frequency during skin development, ...
A Noise Trimming and Positional Significance of
A Noise Trimming and Positional Significance of

... sites from zero to many. An individual site may attract insertions from one to many depending on the coverage depth of sequencing as well as the genetic property of a gene. The number of insertions at the same site is called insertion count or simply count. The significance of mutation of a gene sho ...
Estonian HumanGenesResearchAct.
Estonian HumanGenesResearchAct.

... tissue sample, the description of his or her state of health and his or her genealogy and shall deliver the personal data of the gene donor, the tissue sample, the description of his or her state of health and his or her genealogy to the chief processor or an authorised processor designated by the c ...
The Expression in Staphylococcus aureus of Cloned DNA Encoding
The Expression in Staphylococcus aureus of Cloned DNA Encoding

... al., 1987). Construction of the shuttle vector pGC2 (a hybrid of the S. aureus chloramphenicol-resistance plasmid pC194 opened at its HindIII site and inserted into the PvuII site of the E. coli vector pGEM1) and subcloning into this vector were by standard methods (Maniatis et al., 1982).Strain ANS ...
national senior certificate grade 12
national senior certificate grade 12

... During gamete formation, members of each allele pair separate such that each gamete only contains one allele for a particular trait ...
EA3407770B396A1469256F2D0027A4A8
EA3407770B396A1469256F2D0027A4A8

... Territory but to allow pre-existing research trials to continue in honouring prior arrangements to do so. In terms of agricultural crops, some of the possible risks include the potential impact on traditional or organic crops; the possible effect on insect resistant crops or non-target insects such ...
A novel NUP98/RARG gene fusion in acute myeloid
A novel NUP98/RARG gene fusion in acute myeloid

... abbreviations for Figure 2, “DBD, DNA-binding domain and distribution and arrows the joining site” has been rephrased as “DBD, DNA-binding domain. Arrows indicate the joining sites.” Please confirm or correct. DNA-binding domain. Arrows indicate the joining sites. ...
Document
Document

... Mendel then crossed these second generation tall pea plants and ended up with 1 out 4 being small. ...
source file
source file

... notebook takes you to a different part of the Expasy site – do not use it. ...
DNA
DNA

... – was known to be a chemical in cells by the end of the nineteenth century, – has the capacity to store genetic information, and – can be copied and passed from generation to generation. ...
The use of multiple displacement amplification to amplify complex
The use of multiple displacement amplification to amplify complex

... Paired-End diTags (ChIP-PET) libraries used for elucidating transcription factor binding sites (5). In constructing such libraries, the starting DNA samples are often limited, and therefore DNA amplification is often necessary. The method of choice has been bacterial propagation of DNA fragments in p ...
Cloning and Functional Characterization of a Fatty Acyl
Cloning and Functional Characterization of a Fatty Acyl

... the cDNA identified highly with the FatB class of plant thioesterases. The cDNA contained a 1,245 bp open reading frame (ORF), encoding a protein that contained 414 amino acid residues. Subcellular localization results showed that the BnFatB protein was located in the chloroplast. The BnFatB (KC4452 ...
PPT - Bioinformatics.ca
PPT - Bioinformatics.ca

... – TF binding sites, gene structure (intron/exon), SNPs ...
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Helitron (biology)

A helitron is a transposon found in eukaryotes that is thought to replicate by a so-called ""rolling-circle"" mechanism. This category of transposons was discovered by Vladimir Kapitonov and Jerzy Jurka in 2001. The rolling-circle process begins with a break being made at the terminus of a single strand of the helitron DNA. Transposase then sits at this break and at another break where the helitron targets as a migration site. The strand is then displaced from its original location at the site of the break and attached to the target break, forming a circlular heteroduplex. This heteroduplex is then resolved into a flat piece of DNA via replication. During the rolling-circle process, DNA can be replicated beyond the initial helitron sequence, resulting in the flanking regions of DNA being ""captured"" by the helitron as it moves to a new location.
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