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Module 8: Horizontal Gene Transfer
Module 8: Horizontal Gene Transfer

... 9.3. Once the phylogram is produced in T-Coffee, it can be evaluated to make predictions on whether the gene under investigation might have been transferred to Kytococcus by horizontal gene transfer. However, more informative true phylogenetic trees can be prepared at another site. If your instructo ...
Analysis of Variance of Microarray Data
Analysis of Variance of Microarray Data

... to validate the accuracy of the transcript‐level measurements. They do not provide information about variation in the population. Similarly, replicate probes within an array are designed to limit the need for technical replicates by increasing the confidence of abundance measures for a given target ...
dna - columbusisd.org
dna - columbusisd.org

... • STRs are locations on the chromosome that contain short sequences that repeat themselves within the DNA molecule. • They serve as useful markers for identification because they are found in great abundance throughout the human genome. ...
Detecting multiple DNA human profile from a mosquito blood meal
Detecting multiple DNA human profile from a mosquito blood meal

... We obtained the genetic profiles for three and four mixed samples from a single mosquito 24 h following the blood meal. However, we did not obtain viable results from mixtures found in mosquitos 48 and 72 h following hematophagy. Moreover, we also did not obtain profiles for two mixed samples after ...
Arabidopsis thaliana: A Model Plant for Genome Analysis
Arabidopsis thaliana: A Model Plant for Genome Analysis

Chapter 2. Structures of Nucleic Acids
Chapter 2. Structures of Nucleic Acids

... DNA and RNA are both nucleic acids, which are the polymeric acids isolated from the nucleus of cells. DNA and RNA can be represented as simple strings of letters, where each letter corresponds to a particular nucleotide, the monomeric component of the nucleic acid polymers. Although this conveys alm ...
Educator Guide - Cheryl Bardoe
Educator Guide - Cheryl Bardoe

... Mendel saw a pattern in the results of his pea-breeding experiments. A pattern is a repetitive form, order or arrangement. Patterns can be used to predict what comes next, but only if you understand the rules beneath the pattern. By examining the following sequences, students can identify the rules ...
Mitochondrial DNA SNP Detection: Design Issues and the Use of the
Mitochondrial DNA SNP Detection: Design Issues and the Use of the

... MassEXTEND™ (hME) assay developed by Sequenom (San Diego, CA) (74-76). The assay is based on primer extension and incorporation of ddNTPs (Figure 7). The primer extension allele products, which typically differ by one nucleotide (but could be no more than the mass differences between two primer exte ...
Lecture 8: Gene Finding by Computational Analysis Outline 1
Lecture 8: Gene Finding by Computational Analysis Outline 1

... = total number of occurrences of X divided by total number of dicodon occurrences – Freq of dicodon X (e.g, AAA AAA) in noncoding region = total number of occurrences of X divided by total number of dicodon occurrences Exercise: In human genome, freq of dicodon “AAA AAA” is ~1% in coding region vs ~ ...
Gene Section FANCC (Fanconi anaemia complementation group C) Atlas of Genetics and Cytogenetics
Gene Section FANCC (Fanconi anaemia complementation group C) Atlas of Genetics and Cytogenetics

... Implicated in Fanconi anaemia (FA) FACC is implicated in the FA complementation group C; it represents about 15% of FA cases. Disease Fanconi anaemia is a chromosome instability syndrome/cancer prone disease (at risk of leukaemia). Prognosis Fanconi anaemia's prognosis is poor; mean survival is 16 y ...
Document
Document

... increased in the escitalopramtreated groups • mRNA level of the FSL-Esc group was not statistically different from FRL ...
The novel mutation of CYP21A2 gene and congenital adrenal
The novel mutation of CYP21A2 gene and congenital adrenal

... CYP21A2 gene mutations in patients with classical congenital adrenal hyperplasia from 10 families CYP21A2active gene, (OMIM #201910), is located on chromosome 6p21.3, which is adjacent to a highly homologous pseudogene (CYP21A1P). The homology in exon and intron between CYP21A2 and CYP21A1P is up to ...
Soliton-like base pair opening in a helicoidal DNA: An analogy with
Soliton-like base pair opening in a helicoidal DNA: An analogy with

... to the equations of motion. Experimentally, the life time of soliton in this case is shown to be of few nano seconds at room temperature (see for e.g. Ref.[22]). Also, in a recent paper, Yakushevich et al [23] through a numerical analysis, showed that when the viscosity is strong the soliton moves a ...
Complete mitochondrial genome of a natural triploid
Complete mitochondrial genome of a natural triploid

... ABSTRACT. Carassius auratus var. pingxiangnensis is a natural triploid crucian carp mutant. In order to understand its placement and genetic background at the gene level, the characteristics of mitochondrial DNA sequences and phylogenetic relationship were examined. The results showed that the mitoc ...
SCOOTER OER Fact Sheet: Dr V Rolfe, December 2010
SCOOTER OER Fact Sheet: Dr V Rolfe, December 2010

... Gene expression alters with life-stage and in turn causes different Hb molecules to be synthesised at different times. Which Hb molecules are produced in the embryonic phase? Gower 1, Gower 2 and Portland 1 Gower 1 and Gower 2 Portland 1 and Portland 2 Foetal (HbF) and Gower 1 ...
Archives of Microbiology 167:
Archives of Microbiology 167:

... carry out N2 fixation only when the oxygen tension in the environment is low, e.g., during the dark phase of a natural diel cycle when photosynthetic O2 production ceases and respiratory O2 consumption increases (Gallon 1992). Many filamentous cyanobacteria fix N2 concurrently with photosynthetic O2 ...
The Nucleotide and Derived Amino Acid
The Nucleotide and Derived Amino Acid

... acterized. Southern blot analyses of apo-A-IV gene- significant activator of 1ecithin:cholesterol acyltransferase. containing cosmids revealed that theapo-A-IV gene is This finding is consistent with the structure of rat apo-A-IV linked to the apo-’A-Iand apo-C-I11 geneswithin a 20- that we determin ...
Restriction Enzymes in Microbiology, Biotechnology and
Restriction Enzymes in Microbiology, Biotechnology and

... proteins regulate biochemical processes such as transcription, DNA replication and division, recombination and repair, epigenetic modification, and likely others yet to be discovered. Sequence-recognition is central to many cellular processes, and for the proteins involved it can mean searching amon ...
Shastry, B.S. 2002. SNP alleles in human disease and evolution
Shastry, B.S. 2002. SNP alleles in human disease and evolution

(HPV) L1 gene DNA possibly bound to particulate aluminum
(HPV) L1 gene DNA possibly bound to particulate aluminum

... buffer, 1 μL of BigDye Terminator 1.1 (Applied Biosystems) and 1 μL of 10 μM GP6, or GP5 sequencing primer. After thermal cycling according to the manufacturer's recommendation, the reaction mixture was loaded in an automated ABI 3130 four-capillary Genetic Analyzer for sequence analysis. Alignment ...
RNA Synthesis and Processing
RNA Synthesis and Processing

... • Split genes may also facilitate the evolution of new proteins. • Proteins often have a modular architecture with discrete structural and functional regions called domains. • In many cases, different exons code for different domains of a protein. Copyright © 2002 Pearson Education, Inc., publishin ...
Synthetic Biology
Synthetic Biology

... Building Devices • Devices are themselves parts, but they are built from several smaller components. • The choice of input/output of a device is very important, as it determines how parts can be ‘connected’. ...
CHAPTER 17 FROM GENE TO PROTEIN Section B: The Synthesis
CHAPTER 17 FROM GENE TO PROTEIN Section B: The Synthesis

... • Split genes may also facilitate the evolution of new proteins. • Proteins often have a modular architecture with discrete structural and functional regions called domains. • In many cases, different exons code for different domains of a protein. Copyright © 2002 Pearson Education, Inc., publishin ...
17B-RNASynthesisAndProcess
17B-RNASynthesisAndProcess

... • Split genes may also facilitate the evolution of new proteins. • Proteins often have a modular architecture with discrete structural and functional regions called domains. • In many cases, different exons code for different domains of a protein. Copyright © 2002 Pearson Education, Inc., publishin ...
The molecular epidemiology of iridovirus in Murray cod
The molecular epidemiology of iridovirus in Murray cod

... arbitrarily chosen as a limit. If both primers in the pair had E-values of less than 1.0 for the same non-target DNA, they were excluded. Candidate primers for discriminatory assays, C50/C51 and C82/C83, were tested against DNA from MCIV, DGIV-2004, RSIV and EHNV. Primers (M68/ M69 and M151/M152) kn ...
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Helitron (biology)

A helitron is a transposon found in eukaryotes that is thought to replicate by a so-called ""rolling-circle"" mechanism. This category of transposons was discovered by Vladimir Kapitonov and Jerzy Jurka in 2001. The rolling-circle process begins with a break being made at the terminus of a single strand of the helitron DNA. Transposase then sits at this break and at another break where the helitron targets as a migration site. The strand is then displaced from its original location at the site of the break and attached to the target break, forming a circlular heteroduplex. This heteroduplex is then resolved into a flat piece of DNA via replication. During the rolling-circle process, DNA can be replicated beyond the initial helitron sequence, resulting in the flanking regions of DNA being ""captured"" by the helitron as it moves to a new location.
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