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Molecular Biology Fundamentals
Molecular Biology Fundamentals

... Information coded in DNA (deoxyribonucleic acid) directs the synthesis of different RNA (ribonucleic acid) molecules. RNA molecules fall into several different categories: rRNA: ribosomal RNA that is required for building ribosomes, which are structures necessary for protein synthesis. tRNA: transfe ...
View PDF - CiteSeerX
View PDF - CiteSeerX

... ycfs, however, has been brought into question by the relatively frequent occurrence of pseudo-ycf loci. For example, although ycf15 in tobacco appears to be a potentially functional protein-coding gene, in many other species—including E. globulus—a variable insertion of 250 bp (295 bp in E. globulu ...
attachment of amino acids to tRNA
attachment of amino acids to tRNA

... transfer of AMP to the COO- end of the amino acids. 2. tRNA charging: transfer of the adenylylated amino acids to the 3’ end of tRNA, generating aminoacyl-tRNAs (charged tRNA). ...
Controlling morpholino experiments: don`t stop making antisense
Controlling morpholino experiments: don`t stop making antisense

... [Editorial, Nature Genetics 26(2), 129-130], that MOs could be targeted to knockdown specific gene expression both in frog (Heasman et al., 2000) and in zebrafish (Ekker, 2000; Nasevicius and Ekker, 2000). The power of MOs to test gene function was quickly recognised and applied to other organisms, ...
Nucleic Acid and Protein Quantitation Methods
Nucleic Acid and Protein Quantitation Methods

... detection. For dsDNA, the common methods include Hoechst, and Invitrogen Quant-iT PicoGreen®, Broad Range, and High Sensitivity dsDNA kits. These dyes have different excitation/emission profiles (Table 1) which may be more or less convenient depending on the individual application. Hoechst can be le ...
Emerging Understanding of Minireview
Emerging Understanding of Minireview

... directly recognizes the stop codon at the A site, as has also been found in vitro (Tate and Brown, 1992, and references therein). The P-site tRNA thus seems to constitute another surface of a common A-site pocket for the EF-Tu ternary complex and RF (Figure 3). The mRNA sequence immediately downstre ...
molecularevolution.rnaseq
molecularevolution.rnaseq

... Statistical segmentation of chromatin modifications uses continuity of segments to increase power for interval detection ...
Operon review
Operon review

... INACTIVE (Circle one) INACTIVE (Circle one) ...
Inquiry into Life Twelfth Edition
Inquiry into Life Twelfth Edition

... Defeating Attenuation • Attenuation operates in the E. coli trp operon as long as tryptophan is plentiful • If amino acid supply low, ribosomes stall at the tandem tryptophan codons in the trp leader • trp leader being synthesized as stalling occurs, stalled ribosome will influence the way RNA fold ...
2013年1月12日托福写作真题回忆
2013年1月12日托福写作真题回忆

... For example, an excess of certain proteins can cause cells (15) to proliferate abnormally and become cancerous; a lack of the protein insulin results in diabetes. Biologists once assumed that the variable rates at which cells synthesize different mRNA’s determine the ...
Nucleotide sequence of the 3h-terminal two
Nucleotide sequence of the 3h-terminal two

... Pseudococcus calceolariae (Petersen & Charles, 1997) and Planococcus citri (Cabaleiro & Segura, 1997). In addition, GLRaV-3 has been reported to be transmitted by the scale insect Pulvinaria vitis L. (Belli et al., 1994). We have investigated GLRaV-3 as part of our long-term goal to characterize the ...
Nucleotide sequence of the 3h-terminal two
Nucleotide sequence of the 3h-terminal two

... Pseudococcus calceolariae (Petersen & Charles, 1997) and Planococcus citri (Cabaleiro & Segura, 1997). In addition, GLRaV-3 has been reported to be transmitted by the scale insect Pulvinaria vitis L. (Belli et al., 1994). We have investigated GLRaV-3 as part of our long-term goal to characterize the ...
The Functional Organization of the Vestigial Locus in Drosophila
The Functional Organization of the Vestigial Locus in Drosophila

... (1981b) have proposed that the cell death observed in vg mutants may be the consequence of abnormal positional information in vg wing imaginal discs. Thus, a molecular analysis of vg is important since it may help to elucidate the mechanisms by which positional information in imaginal discs is estab ...
ist 480: molecular dioagnostics - MU BERT
ist 480: molecular dioagnostics - MU BERT

... http://www.marshall.edu/catalog/undergraduate/ug_10-11_published.pdf. Students without a valid excuse will receive an F (zero) for the exam. Quizzes: There will be several quizzes during the semester. The quiz dates will be announced in advance to allow for adequate preparation. The quizzes may vary ...
ist 480: molecular dioagnostics - MU BERT
ist 480: molecular dioagnostics - MU BERT

... undergraduate catalog: http://www.marshall.edu/catalog/undergraduate/ug_10-11_published.pdf. Students without a valid excuse will receive an F (zero) for the exam. Quizzes: There will be several quizzes during the semester. The quiz dates will be announced in advance to allow for adequate preparatio ...
Bioinformatics 3 V7 * Function Annotation, Gene Regulation
Bioinformatics 3 V7 * Function Annotation, Gene Regulation

... Left: Protein – DNA contacts involve many arginine (R) and lysine (K) residues ...
1. Cellular control Booklet TN
1. Cellular control Booklet TN

... DO NOT CREDIT descriptions that contains errors 2. transcription/transcribed; 3. one strand copied; ACCEPT coding/sense/non-sense/template, strand (implying one only) 4. complementary base-pairing; CREDIT description of base pairing as correct to context 5. triplet code/code read in threes/codon is ...
Novel Function of the Eukaryotic Polypeptide
Novel Function of the Eukaryotic Polypeptide

... translational accuracy in yeast. However, the relationship between the yeast cell-cycle control and translational regulation by the GSPT/SUP35 gene product remained to be determined. We previously cloned a human homologue of the yeast GSPT gene and two mouse GSPT genes, the counterpart of human GSPT ...
A newly discovered human -globin gene
A newly discovered human -globin gene

... The globin genes and their products have been intensively investigated for the past 50 years. Those studies led to the description of structural and regulatory elements that are useful for the recognition and comparison of hundreds of globin gene family members. The divergence of ancestral ␣- and ␤- ...
G O N I O S C O P Y M A D E E A S Y
G O N I O S C O P Y M A D E E A S Y

... • Antivirals are useful for treatment of influenza and as a secondary means of preventing influenza • Antivirals should be used where they have the greatest benefit • Antivirals should be used responsibly in order to minimise the risk of emergence of drug resistance • It is important that there is a ...
Advancing Science with DNA Sequence Finding the genes in
Advancing Science with DNA Sequence Finding the genes in

... genome in Artemis genome viewer: Sequence features in prokaryotic genomes:  stable RNA-coding genes (rRNAs, tRNAs, RNA component of RNaseP, tmRNA)  protein-coding genes (CDSs)  transcriptional features (mRNAs, operons, promoters, terminators, protein-binding sites, DNA bends)  translational feat ...
synthesis Gene Cluster of Streptomyces clavuligerus
synthesis Gene Cluster of Streptomyces clavuligerus

... the existence of an ArgR-mediated control of arginine biosynthesis gene expression. A low level constitutive monocistronic transcript of argR was observed in S. clavuligerus cells. Most of the argGH transcript initiating at an adenine 29 nt upstream of the argG initiation codon appears to stop at a ...
-Chain Gene in Epididymis α Expression of the C4b
-Chain Gene in Epididymis α Expression of the C4b

... C4b-binding protein (C4BP) is a large plasma protein composed of seven ␣-chains and one ␤-chain and is involved in the fluid phase regulation of the classical pathway of the complement system. Complement inhibitory activity is located in the ␣-chain, and its mRNA has been detected only in liver to d ...
Temporal Control of Gene Silencing by in ovo Electroporation
Temporal Control of Gene Silencing by in ovo Electroporation

... induction of loss-of-function phenotypes. Unlike adult tissue or cell lines, embryos do not respond to long dsRNA with unspecific inhibition of protein synthesis and apoptosis (18,19). Therefore, there is no need for lengthy selection of an efficient siRNA or shRNA. Any cDNA fragment or expressed se ...
From Molecular Systems to Simple Cells: a - TBI
From Molecular Systems to Simple Cells: a - TBI

... In any living organism, phenotype refers to the physical, organizational and behavioral expression during its lifetime. Genotype refers to a heritable repository of information that instructs the production of molecules whose interactions, in conjunction with the environment, generate and maintain t ...
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Non-coding RNA



A non-coding RNA (ncRNA) is an RNA molecule that is not translated into a protein. Less-frequently used synonyms are non-protein-coding RNA (npcRNA), non-messenger RNA (nmRNA) and functional RNA (fRNA). The DNA sequence from which a functional non-coding RNA is transcribed is often called an RNA gene.Non-coding RNA genes include highly abundant and functionally important RNAs such as transfer RNAs (tRNAs) and ribosomal RNAs (rRNAs), as well as RNAs such as snoRNAs, microRNAs, siRNAs, snRNAs, exRNAs, and piRNAs and the long ncRNAs that include examples such as Xist and HOTAIR (see here for a more complete list of ncRNAs). The number of ncRNAs encoded within the human genome is unknown; however, recent transcriptomic and bioinformatic studies suggest the existence of thousands of ncRNAs., but see Since many of the newly identified ncRNAs have not been validated for their function, it is possible that many are non-functional. It is also likely that many ncRNAs are non functional (sometimes referred to as Junk RNA), and are the product of spurious transcription.
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