Biomart/ GENOME ALIGNMENT III
... The comparison of the mouse and human genomes has demonstrated the power of comparative genomics in inferring the evolutionary history of species and in identifying functional regions in genomes. The possibilities for identifying regions under selection are enhanced with the addition of more sequenc ...
... The comparison of the mouse and human genomes has demonstrated the power of comparative genomics in inferring the evolutionary history of species and in identifying functional regions in genomes. The possibilities for identifying regions under selection are enhanced with the addition of more sequenc ...
Perspective Two empires or three?
... original Hennigian principles, sister groups must always be given the same categorical rank. Hence the archaebacteria must be removed from the prokaryotes and given the same high rank as the eukaryotes. Woese does not seem to realize that this sister group ranking principle of Hennig had led to so m ...
... original Hennigian principles, sister groups must always be given the same categorical rank. Hence the archaebacteria must be removed from the prokaryotes and given the same high rank as the eukaryotes. Woese does not seem to realize that this sister group ranking principle of Hennig had led to so m ...
Programmed Ribosomal Frameshifting Generates a Copper
... copA mRNA showed no difference from the DNA sequence. Having excluded nucleotide sequence alteration as a source of the CopA(Z) C-terminal amino acid change, we explored other scenarios that might lead to the synthesis of a truncated protein with an aberrant amino acid sequence. Close inspection of ...
... copA mRNA showed no difference from the DNA sequence. Having excluded nucleotide sequence alteration as a source of the CopA(Z) C-terminal amino acid change, we explored other scenarios that might lead to the synthesis of a truncated protein with an aberrant amino acid sequence. Close inspection of ...
Multiplex in-vitro Detection using SERS
... beneficial in obtaining further enhancement in the form of surface enhanced resonance Raman scattering (SERRS). This occurs when the analyte contains a chromophore close in energy to the exciting radiation. However, a dye label can also be used to achieve this enhancement if the chromophore is not p ...
... beneficial in obtaining further enhancement in the form of surface enhanced resonance Raman scattering (SERRS). This occurs when the analyte contains a chromophore close in energy to the exciting radiation. However, a dye label can also be used to achieve this enhancement if the chromophore is not p ...
Nucleotide sequence and taxonomic value of the
... program DNAPARS and the program DNADIST, which computes the 'distance' between pairs of species from DNA sequences which are then utilized by the distance matrix programs FITCH and KITSCH. Sequences were first aligned with the multiple alignment program CLUSTAL (Higgins & Sharp, 1988) so that they c ...
... program DNAPARS and the program DNADIST, which computes the 'distance' between pairs of species from DNA sequences which are then utilized by the distance matrix programs FITCH and KITSCH. Sequences were first aligned with the multiple alignment program CLUSTAL (Higgins & Sharp, 1988) so that they c ...
Nanopore Unzipping of Individual DNA Hairpin Molecules
... under constant force or constant loading rate. Using a dynamic voltage control method, the entry rate of polynucleotides into the pore and the voltage pattern applied to induce hairpin unzipping are independently set. Thus, hundreds of unzipping events can be tested in a short period of time (few mi ...
... under constant force or constant loading rate. Using a dynamic voltage control method, the entry rate of polynucleotides into the pore and the voltage pattern applied to induce hairpin unzipping are independently set. Thus, hundreds of unzipping events can be tested in a short period of time (few mi ...
pyrimidine
... Uses short primer that attaches to the 3’ end of the ssDNA, after which a specially engineered DNA polymerase Each vial includes one dideoxyXTP and 3 ordinary dXTPs; the dideoxyXTP will be incorporated but will halt synthesis because the 3’ position is blocked. See figs. 11.3 & 11.4 for how these ar ...
... Uses short primer that attaches to the 3’ end of the ssDNA, after which a specially engineered DNA polymerase Each vial includes one dideoxyXTP and 3 ordinary dXTPs; the dideoxyXTP will be incorporated but will halt synthesis because the 3’ position is blocked. See figs. 11.3 & 11.4 for how these ar ...
Par-1
... machinery Many components of RNAi machinery have been identified through genetic screening for RNAi defective mutants and through biochemical studies using cell extracts (e.g. Drosophila embryo extract). ...
... machinery Many components of RNAi machinery have been identified through genetic screening for RNAi defective mutants and through biochemical studies using cell extracts (e.g. Drosophila embryo extract). ...
Cormack et al, 1991 Cell
... not the divergent N-terminal regions. The N-terminal region Is unimportant for the essential function(s) of yeast TFIID because expression of the core domain permits eff icient cell growth. Analysis of yeast-human hybrid TFllDs indicates that several regions within the conserved core account for the ...
... not the divergent N-terminal regions. The N-terminal region Is unimportant for the essential function(s) of yeast TFIID because expression of the core domain permits eff icient cell growth. Analysis of yeast-human hybrid TFllDs indicates that several regions within the conserved core account for the ...
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... nitrogen sink. Although denitrification has long been believed to be the major process responsible for fixed nitrogen loss from the oceans, recent studies show that anaerobic ammonium oxidation (anammox) is potentially a more important process involved. We have investigated the phylogeny of both ana ...
... nitrogen sink. Although denitrification has long been believed to be the major process responsible for fixed nitrogen loss from the oceans, recent studies show that anaerobic ammonium oxidation (anammox) is potentially a more important process involved. We have investigated the phylogeny of both ana ...
Real time PCR and it`s role in diagnosis
... Improving our understanding of the biology of the Plasmodium falciparum parasite is of extreme importance if we are to combat human malaria.This parasite uses the process of antigenic variation to expose the human immune system to continually changing antigens on the surface of infected red blood ce ...
... Improving our understanding of the biology of the Plasmodium falciparum parasite is of extreme importance if we are to combat human malaria.This parasite uses the process of antigenic variation to expose the human immune system to continually changing antigens on the surface of infected red blood ce ...
A genome-wide analysis of DNA methylation in buccal - VU-DARE
... been identified, although for most of these variants the biological mechanisms remain to be elucidated 1. Interestingly, the majority of disease-associated genetic variation is located in regulatory regions of the genome 2, including transcription-factor-occupied regions and DNase I hypersensitive s ...
... been identified, although for most of these variants the biological mechanisms remain to be elucidated 1. Interestingly, the majority of disease-associated genetic variation is located in regulatory regions of the genome 2, including transcription-factor-occupied regions and DNase I hypersensitive s ...
Novel regulatio pendage transformation
... EN and INV. Partial cDNAs of two engrailed-class genes have been cloned from P. scaber, both of them contain the epitope and are expressed in coinciding patterns (A. A. and T. C. K., unpublished). The EN expression pattern is similar to that observed in insects and other crustaceans at the segmented ...
... EN and INV. Partial cDNAs of two engrailed-class genes have been cloned from P. scaber, both of them contain the epitope and are expressed in coinciding patterns (A. A. and T. C. K., unpublished). The EN expression pattern is similar to that observed in insects and other crustaceans at the segmented ...
Modified uridine at wobble position in tRNA of
... polymerase III (Sprague, 1995). The precursor tRNAs undergo a series of posttranscriptional processing events to generate a mature form. These events include (1) cleavage of the 5' leader by Ribonuclease P (RNase P) (Darr et al., 1992; Altman et al, 1995); (2) removal of the 3' trailer sequence by e ...
... polymerase III (Sprague, 1995). The precursor tRNAs undergo a series of posttranscriptional processing events to generate a mature form. These events include (1) cleavage of the 5' leader by Ribonuclease P (RNase P) (Darr et al., 1992; Altman et al, 1995); (2) removal of the 3' trailer sequence by e ...
Biology Final Exam Review Questions Answer Section SHORT
... TOP: Foundation Edition BLM: application 30. ANS: The nucleotides in a strand of DNA are joined by covalent bonds between their sugar and phosphate groups, and by hydrogen bonds between the complimentary bases. PTS: 1 DIF: L3 REF: p. 344 OBJ: 12.2.1 Identify the chemical components of DNA. STA: CA.B ...
... TOP: Foundation Edition BLM: application 30. ANS: The nucleotides in a strand of DNA are joined by covalent bonds between their sugar and phosphate groups, and by hydrogen bonds between the complimentary bases. PTS: 1 DIF: L3 REF: p. 344 OBJ: 12.2.1 Identify the chemical components of DNA. STA: CA.B ...
Myriad--Ambry -- Final Version of Ambry Preliminary Injunction
... “primers” and “probes” that are complementary to a particular genomic target sequence. Synthetically created complementary DNA molecules are different from genomic DNA because they are not naturally occurring. Rather, they are synthetic, laboratory-created DNA carefully designed by man to achieve sp ...
... “primers” and “probes” that are complementary to a particular genomic target sequence. Synthetically created complementary DNA molecules are different from genomic DNA because they are not naturally occurring. Rather, they are synthetic, laboratory-created DNA carefully designed by man to achieve sp ...
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... somite they will form and which set of genes they will express during differentiation (Pourquié, 2011). The spatiotemporal dynamics of oscillations create a phase difference among cells along the PSM. Anterior PSM cells are retarded in the oscillator phase relative to posterior PSM cells; the furthe ...
... somite they will form and which set of genes they will express during differentiation (Pourquié, 2011). The spatiotemporal dynamics of oscillations create a phase difference among cells along the PSM. Anterior PSM cells are retarded in the oscillator phase relative to posterior PSM cells; the furthe ...
Maintenance of genomic integrity by p53: complementary
... can modify the sequence-speci®c DNA binding activity of p53 and may thereby modulate the relative eciency of activation of dierent p53 target genes (Hecker et al., 1996; Hupp and Lane, 1994; Hupp et al., 1992; Takenaka et al., 1995; Wang and Prives, 1995). Phosphorylation by DNA-PK within the tran ...
... can modify the sequence-speci®c DNA binding activity of p53 and may thereby modulate the relative eciency of activation of dierent p53 target genes (Hecker et al., 1996; Hupp and Lane, 1994; Hupp et al., 1992; Takenaka et al., 1995; Wang and Prives, 1995). Phosphorylation by DNA-PK within the tran ...
Synapsis-Mediated Fusion of Free DNA Ends Forms Inverted Dimer Plasmids in Yeast.
... the other hand, join end-to-end in an apparently nonhomologous reaction (ORR-WEAVERand SZOSTAK 1983; SUZUKIet al. 1983). Such end-to-end joining products arerecovered exclusively on transformation with a plasmid linearized in region lacking homology with the yeast genome (KUNES, BOTSTEINand Fox 1985 ...
... the other hand, join end-to-end in an apparently nonhomologous reaction (ORR-WEAVERand SZOSTAK 1983; SUZUKIet al. 1983). Such end-to-end joining products arerecovered exclusively on transformation with a plasmid linearized in region lacking homology with the yeast genome (KUNES, BOTSTEINand Fox 1985 ...
Multiple Roles of the Y Chromosome in the Biology of
... It is generally accepted that the sex chromosomes of most animals arose from a couple of homologous autosomes after the acquisition of a dominant sex determination gene[17,18] and that the Y chromosome is the result of the degeneration of one of them[19,20,21]. Its degeneration would be a consequenc ...
... It is generally accepted that the sex chromosomes of most animals arose from a couple of homologous autosomes after the acquisition of a dominant sex determination gene[17,18] and that the Y chromosome is the result of the degeneration of one of them[19,20,21]. Its degeneration would be a consequenc ...
Repression by RAZ of Epstein-Barr virus bZIP transcription factor
... increasing amounts, the amount of FLAG-RAZ protein was far less than the amount of EB1 protein, even in the transfections where the amount of EB1 was reduced (Fig. 2c, compare mAbZ125 with mAbM2, lane 5). It should be stressed that 5,1 of total cell protein extract was used to visualize the amount o ...
... increasing amounts, the amount of FLAG-RAZ protein was far less than the amount of EB1 protein, even in the transfections where the amount of EB1 was reduced (Fig. 2c, compare mAbZ125 with mAbM2, lane 5). It should be stressed that 5,1 of total cell protein extract was used to visualize the amount o ...
Robust gene silencing mediated by antisense small RNAs in the
... sRNAs in E. histolytica. We show for the first time that AS sRNAs directly mediate gene silencing. Our data demonstrate that a small portion of a gene-coding region to which large numbers of AS sRNAs map is sufficient to ‘trigger’ silencing of a gene fused to it. Gene silencing only occurred in E. his ...
... sRNAs in E. histolytica. We show for the first time that AS sRNAs directly mediate gene silencing. Our data demonstrate that a small portion of a gene-coding region to which large numbers of AS sRNAs map is sufficient to ‘trigger’ silencing of a gene fused to it. Gene silencing only occurred in E. his ...
Left-right axis asymmetry determining human Cryptic gene is
... Although there are reports demonstrating specific expression patterns of Cryptic gene in the mouse-embryos and adults, information about its transcriptional regulation and controlled expression is missing [13–15]. In order to identify the transcription factors that might bind to the promoter region ...
... Although there are reports demonstrating specific expression patterns of Cryptic gene in the mouse-embryos and adults, information about its transcriptional regulation and controlled expression is missing [13–15]. In order to identify the transcription factors that might bind to the promoter region ...
Primary transcript
A primary transcript is the single-stranded ribonucleic acid (RNA) product synthesized by transcription of DNA, and processed to yield various mature RNA products such as mRNAs, tRNAs, and rRNAs. The primary transcripts designated to be mRNAs are modified in preparation for translation. For example, a precursor messenger RNA (pre-mRNA) is a type of primary transcript that becomes a messenger RNA (mRNA) after processing.There are several steps contributing to the production of primary transcripts. All these steps involve a series of interactions to initiate and complete the transcription of DNA in the nucleus of eukaryotes. Certain factors play key roles in the activation and inhibition of transcription, where they regulate primary transcript production. Transcription produces primary transcripts that are further modified by several processes. These processes include the 5' cap, 3'-polyadenylation, and alternative splicing. In particular, alternative splicing directly contributes to the diversity of mRNA found in cells. The modifications of primary transcripts have been further studied in research seeking greater knowledge of the role and significance of these transcripts. Experimental studies based on molecular changes to primary transcripts the processes before and after transcription have led to greater understanding of diseases involving primary transcripts.