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Slides
Slides

... • left = bi-partite graph for GO terms (yellow -> red scale) and miRNA (grey) • edge is enrichment of the GO term in the set of genes most correlated with the miRNA. • right = GO summary network for GO terms in the left enrichment network. Each GO nodes color-coded by cumulative enrichment score for ...
Behavioral Evolution and Altruism
Behavioral Evolution and Altruism

... In fact, mutant alleles of a gene known as fruitless, expressed in fruit fly brain cells, cause males to be unable to recognize females (and court each other!). Female flies with fruitless ...
Document
Document

... • Involves a change in the number or structure of the chromosomes. • Deletion : when a piece of a chromosome breaks off and is lost. • Duplication : when a segment of a chromosome is repeated • Inversion : when a segment of a chromosome is reversed. ...
TPJ_4378_sm_FigS1-7
TPJ_4378_sm_FigS1-7

... the ninth exon. Exons are represented by boxes and introns by black lines. Black and white boxes indicate coding and non-coding regions, respectively. The start and stop codons are indicated. The numbers indicate nucleotide positions. (b) Reverse-transcription polymerase chain reaction (RT-PCR) anal ...
Document
Document

... a. Structural genes are transcribed into mRNA, but regulator genes are not. b. Structural genes have complex structures; regulator genes have simple structure. c. Structural genes encode proteins that function in the structure of the cell; regulator genes carry out metabolic ...
Bioinformatic Analysis: Designing primers and annotation gene of
Bioinformatic Analysis: Designing primers and annotation gene of

... Bioinformatic Analysis: Designing primers and annotation gene of interest Search for the DNA sequence of the Aiptasia or Symbiodinium gene  WHERE is the best place to try and find the DNA sequence for the species you want to use (in our case, Aiptasia or Symbiodinium)?  You already know about Aipt ...
Chapter 10 (Sample questions)
Chapter 10 (Sample questions)

... The sequence of bases on one strand of DNA could determine the a. sequence of bases in mRNA b. sequence of amino acids in a protein molecule c. sequence of bases in the other DNA strand d. all of the above choices are correct e. a and b are correct How does a cell use its DNA to create the cell itse ...
A) Describe and/or predict observed patterns of
A) Describe and/or predict observed patterns of

... 2. Hemophilia is an inheritable genetic disorder that prohibits the proper formation of blood clots. The recessive gene that causes hemophilia is located on the X-chromosome. Given this information, which of the following statements is true? a. In order for a male offspring to be a hemophiliac, his ...
File - Mr Andrews` Science Space!
File - Mr Andrews` Science Space!

... • • the continuity of life based on the inheritable nature of DNA • • links between DNA and variation in phenotypes • • variation in phenotypes as adaptive features. • 6 Biological concepts and processes relating to the inheritable nature of DNA will be selected from: • • the roles of DNA in both ca ...
mRNA Expression Analysis
mRNA Expression Analysis

... Illumina sequencing determines several billion bases (gigabases, Gb) of sequence data per week. As a result of the massive output rates, this technology offers unparalleled benefits for discovery-stage applications. Nearly any poly-A transcript isoform in any species can be identified and quantified ...
Principles_of_Genetic_engineering
Principles_of_Genetic_engineering

Genetic Principles
Genetic Principles

... • The probability of a fit this good by chance is .00007 • Possible that Mendel’s sample size was larger than he reported. ...
Unit 3: Genetics
Unit 3: Genetics

...  Genes can be removed, altered, and reinserted into an embryo in vitro  Genes can be modified or duplicated with the help of growth medium ...
Chromosomes & Inheritance
Chromosomes & Inheritance

... position of three fruit fly genes, body color (b), wing size (vg), and eye color (cn). • The recombination frequency between cn and b is 9%. • The r.f. between cn and vg is 9.5%. • The r.f. between b and vg is 17%. ...
PowerPoint
PowerPoint

... Software for EBArrays is available at http://www.biostat.wisc.edu/~kendzior. ...
RNA polymerase
RNA polymerase

... Eukaryotic cells modify mRNA after transcription. Splicing of mRNA increases the number of different proteins an organism can produce. Gene expression is regulated by proteins that bind to specific base sequences in DNA. The environment of a cell and of an organism has an impact on gene expression. ...
Gene Section KLLN (killin, p53-regulated DNA replication inhibitor) Atlas of Genetics and Cytogenetics
Gene Section KLLN (killin, p53-regulated DNA replication inhibitor) Atlas of Genetics and Cytogenetics

... an increased risk of breast and renal cancer compared to PTEN mutation positive patients. Methylation leads to a 250-fold decrease in KLLN expression (Bennett et al., 2010). Germline KLLN promoter methylation has been observed in 56% of patients with apparently sporadic renal cell carcinoma (Bennett ...
tggccatcgtaaggtgcgacc ggtagca
tggccatcgtaaggtgcgacc ggtagca

... 2. In the space below, come up with your own metaphor to show the relationship between DNA, genes, and chromosomes. Draw a picture in the space below. Underneath each picture, give a brief description of how your picture represents the concept. ...
histoneHMM (Version 1.5)
histoneHMM (Version 1.5)

Genetics
Genetics

... There is not only a change to one of or several genes, but a change in the total chromosome number or structure. The most famous example is Down's syndrome, in which there is too many of the same chromosome. Instead of a pair of chromosome No. 21 there are three chromosomes 21 in the cells, that is ...
Ch7 microbgeneticspart1HOLrg
Ch7 microbgeneticspart1HOLrg

... to the 3′ end of one Okazaki fragment, it encounters the 5′ end of another. A different type of DNA polymerase then removes the RNA primer nucleotides and simultaneously replaces them with deoxynucleotides. ...
CHAPTER 17
CHAPTER 17

... transcription has been terminated. C28. Answer: RNA interference refers to the phenomenon in which the presence of a double-stranded RNA molecule leads to the silencing of a complementary mRNA. For this to occur, the double-stranded RNA is processed by Dicer into small RNA fragments (miRNA or siRNA) ...
IBC Protocol Review Checklist
IBC Protocol Review Checklist

... transgene under control of gammaretroviral LTR, and progeny is not expected to contain >1/2 exogenous eukaryotic virus genome Considerations for Assessing Risk in the Biological Research Laboratory Review of rDNA and Biosafety protocols submitted to the IBC should include a risk assessment of the bi ...
Molecular Biology – Final Laboratory Report
Molecular Biology – Final Laboratory Report

... Sexual reproduction in Tetrahymena thermophila is a process called conjugation, which all ciliates use. The cell contains two nuclei: a micronucleus used for conjugation and a macronucleus used for transcription (Malone et al. 2008). The process involves the creation and deletion of nearly whole ge ...
Slide 1
Slide 1

... Sequencing technology overview - Two different systems on campus: Illumina GAIIx, 454 - A similar overall strategy for highly-parallel sequencing - Different approaches taken at virtually every step - These different platforms produce data with different characteristics - Other platforms are availa ...
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RNA-Seq



RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.
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