CHAPTER 15 THE CHROMOSOMAL BASIS OF INHERITANCE
... • When half the progeny are recombinants, there is a 50% frequency of recombination. • A 50% frequency of recombination usually indicates that the two genes are on different chromosomes, because it is the expected result if the two genes assort randomly. • The genes for seed shape and seed color ass ...
... • When half the progeny are recombinants, there is a 50% frequency of recombination. • A 50% frequency of recombination usually indicates that the two genes are on different chromosomes, because it is the expected result if the two genes assort randomly. • The genes for seed shape and seed color ass ...
Tuning Biphenyl Dioxygenase for Extended Substrate Specificity
... 1986; Bopp, 1986). Due to its broad substrate specificity, it was used as the starting template for generating variant enzymes that can act on an extended set of PCB congeners. Because substrate specificity is determined mainly by the bphA region, only this gene fragment was subjected to directed ev ...
... 1986; Bopp, 1986). Due to its broad substrate specificity, it was used as the starting template for generating variant enzymes that can act on an extended set of PCB congeners. Because substrate specificity is determined mainly by the bphA region, only this gene fragment was subjected to directed ev ...
Physical Mapping of a 670-kb Region of Chromosomes XVI and XVII
... TEUF0537, and TEUF0056 hybridized only with band XVII (Table 1). These results could be explained by the presence of nonhomologous chromosomes in both bands XVI and XVII. To test this hypothesis, the chromosomal bands XVI and XVII were digested with NotI and hybridized with a T. cruzi telomeric sequ ...
... TEUF0537, and TEUF0056 hybridized only with band XVII (Table 1). These results could be explained by the presence of nonhomologous chromosomes in both bands XVI and XVII. To test this hypothesis, the chromosomal bands XVI and XVII were digested with NotI and hybridized with a T. cruzi telomeric sequ ...
515-527 - CiteSeerX
... and Meyerowitz 1991; Weigel and Meyerowitz 1994). Both of these species, like most other species of eudicots, have flowers that consist of the four floral organ types mentioned above organized in four concentric whorls. Thus, although more than 100 million years separates these two species, their fl ...
... and Meyerowitz 1991; Weigel and Meyerowitz 1994). Both of these species, like most other species of eudicots, have flowers that consist of the four floral organ types mentioned above organized in four concentric whorls. Thus, although more than 100 million years separates these two species, their fl ...
Molecular Characterization of CDC42, a Saccharomyces cerevisiae
... shown), indicating that the complementation of cdc42-1 was due to the autonomously replicating recombinant plasrnids. Restriction enzyme analyses and D N A - D N A blot hybridization experiments (data not shown) indicated that all 24 plasmids contained overlapping regions of DNA. Several representat ...
... shown), indicating that the complementation of cdc42-1 was due to the autonomously replicating recombinant plasrnids. Restriction enzyme analyses and D N A - D N A blot hybridization experiments (data not shown) indicated that all 24 plasmids contained overlapping regions of DNA. Several representat ...
Annex 1
... 30. All data elements defined in paragraph 47, not mentioned in paragraphs 24 to 29, above, are optional (optional data elements). ...
... 30. All data elements defined in paragraph 47, not mentioned in paragraphs 24 to 29, above, are optional (optional data elements). ...
Genetic diversity and epidemiology of infectious hematopoietic
... space, time, and within a host species. The 2 techniques used in this study to genetically characterize the IHNV genes were ribonuclease protection assay (RPA) and nucleotide sequencing. RPA produces a 'genetic fingerprint' of each region of the genome studied. The advantage of RPA is its ability to ...
... space, time, and within a host species. The 2 techniques used in this study to genetically characterize the IHNV genes were ribonuclease protection assay (RPA) and nucleotide sequencing. RPA produces a 'genetic fingerprint' of each region of the genome studied. The advantage of RPA is its ability to ...
Manipulating Yeast Genome Using Plasmid Vectors. In: Gene Expression Technology.
... we discuss below require that one be able to find the rare cells that have undergone a recombination or loss event to remove the plasmid DNA sequences. Unfortunately, the L YS2 gene is quite large, and contains sites within the coding sequence for many of the most common enzymes used in plasmid cons ...
... we discuss below require that one be able to find the rare cells that have undergone a recombination or loss event to remove the plasmid DNA sequences. Unfortunately, the L YS2 gene is quite large, and contains sites within the coding sequence for many of the most common enzymes used in plasmid cons ...
The somatic-visceral subdivision of the embryonic mesoderm is
... carrying the 2xPE-sna fusion gene (sna−; 2xPE-sna) exhibit narrowed limits of twi expression in ventral regions (Fig. 3B; compare with A). Thus, narrowing the limits of the sna pattern cause a corresponding narrowing of the twi pattern. This observation is consistent with the finding that twi expres ...
... carrying the 2xPE-sna fusion gene (sna−; 2xPE-sna) exhibit narrowed limits of twi expression in ventral regions (Fig. 3B; compare with A). Thus, narrowing the limits of the sna pattern cause a corresponding narrowing of the twi pattern. This observation is consistent with the finding that twi expres ...
Human Ig heavy chain CDR3 regions in adult
... than those found in the adult, which can be partly explained by distribution of N regions. Up to 25% of human fetal Ig H chain genes lack N-regional diversity and the N regions that are generated in the remainder of fetal VH–DH–JH junctions are generally smaller than those in adult Ig H chain genes ...
... than those found in the adult, which can be partly explained by distribution of N regions. Up to 25% of human fetal Ig H chain genes lack N-regional diversity and the N regions that are generated in the remainder of fetal VH–DH–JH junctions are generally smaller than those in adult Ig H chain genes ...
Document
... Polymerase chain reaction technique •Presence of product = transgene promoter DNA is in sample ...
... Polymerase chain reaction technique •Presence of product = transgene promoter DNA is in sample ...
“Genes” and “Mutations” - Native American Cancer Research
... There are frequent mutations in cancer cells Sometimes there are large rearrangements Sometimes there are deletions of part of a chromosome Sometimes there are small changes that may only be detected with PCR or other genetic tests. NOTE: PCR stands for polymerase chain reaction. This is the method ...
... There are frequent mutations in cancer cells Sometimes there are large rearrangements Sometimes there are deletions of part of a chromosome Sometimes there are small changes that may only be detected with PCR or other genetic tests. NOTE: PCR stands for polymerase chain reaction. This is the method ...
Gene regulation in three dimensions
... taking the time to answer my questions. Last, but not least I would like to thank Julie my fiancé who has supported me and been there for me when I needed it the most. K.T.W ...
... taking the time to answer my questions. Last, but not least I would like to thank Julie my fiancé who has supported me and been there for me when I needed it the most. K.T.W ...
Triple-hit lymphoma
... follicular lymphoma or BL, respectively. However, to have two gene rearrangements (double-hit lymphomas) is uncommon (2). Translocation of the BCL-2 gene on chromosome 18q21 results in constant inactivation of apoptosis (3). Translocation of the c-MYC gene on chromosome 8q24 results in constant cell ...
... follicular lymphoma or BL, respectively. However, to have two gene rearrangements (double-hit lymphomas) is uncommon (2). Translocation of the BCL-2 gene on chromosome 18q21 results in constant inactivation of apoptosis (3). Translocation of the c-MYC gene on chromosome 8q24 results in constant cell ...
VI. The relationship between genotype and phenotype is rarely simple
... – a ratio of 3:1. The inheritable factor for white flowers was not lost, so the hypothesis was rejected. Conclusions: From these types of experiments and observations, Mendel concluded that since the inheritable factor for white flowers was not lost in the F1 generation, it must have been masked by ...
... – a ratio of 3:1. The inheritable factor for white flowers was not lost, so the hypothesis was rejected. Conclusions: From these types of experiments and observations, Mendel concluded that since the inheritable factor for white flowers was not lost in the F1 generation, it must have been masked by ...
Ugandan Kaposi`s Sarcoma-Associated Herpesvirus Phylogeny
... Viral genomic DNA was isolated from white blood cells collected from 56 patients presenting with KS at the Uganda Cancer Institute in Kampala between June and August 2001. The blood samples were initially collected for routine laboratory evaluation of patients undergoing chemotherapy. Written consen ...
... Viral genomic DNA was isolated from white blood cells collected from 56 patients presenting with KS at the Uganda Cancer Institute in Kampala between June and August 2001. The blood samples were initially collected for routine laboratory evaluation of patients undergoing chemotherapy. Written consen ...
Identification of enzymes involved in anaerobic benzene
... sequence length of the contigs ranged between 0.064 and 537.470 kb. BLASTP search of the translated ORFs indicated that 21.3% of the total identified ORFs could be assigned with more than 50% identity to genes in the NCBI non-redundant protein sequence database, and around 40.7% of the total ORFs di ...
... sequence length of the contigs ranged between 0.064 and 537.470 kb. BLASTP search of the translated ORFs indicated that 21.3% of the total identified ORFs could be assigned with more than 50% identity to genes in the NCBI non-redundant protein sequence database, and around 40.7% of the total ORFs di ...
EPICENTRE Enzyme Catalog
... 6,127,155 and claims outside the US corresponding to US Patent No. 4,889,818. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any oth ...
... 6,127,155 and claims outside the US corresponding to US Patent No. 4,889,818. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any oth ...
Molecular cloning, characterization and gene expression of an
... 2.9. Catalase enzyme activity and functional properties of MrCat Catalase enzyme activity assay and their functional properties of recombinant MrCat protein (rMrCat) experiments were carried out according to Aebi [55] with slight modifications [15]. Briefly, catalase enzyme activity was evaluated by t ...
... 2.9. Catalase enzyme activity and functional properties of MrCat Catalase enzyme activity assay and their functional properties of recombinant MrCat protein (rMrCat) experiments were carried out according to Aebi [55] with slight modifications [15]. Briefly, catalase enzyme activity was evaluated by t ...
The Advantage and Application of Genetically Humanized Mouse
... cDNA was placed under control of a small heterologous promoter to drive ubiquitous or tissue-specific transgene expression and inserted randomly into the genome. Alternatively, cDNA containing the human transgene and small promoter fragment can be targeted to a specific location within the genome. S ...
... cDNA was placed under control of a small heterologous promoter to drive ubiquitous or tissue-specific transgene expression and inserted randomly into the genome. Alternatively, cDNA containing the human transgene and small promoter fragment can be targeted to a specific location within the genome. S ...
A Pneumocystis carinii multi-gene family with
... Four recombinant phage isolated from the cDNA library were partially characterized. The recombinant DNA was recovered from the 1 phage by in vivo excision as pBluescript plasmid DNA. The size of the recombinant DNA ranged from 2-7kb to 2.9 kb, and sequence analysis revealed that all four clones cont ...
... Four recombinant phage isolated from the cDNA library were partially characterized. The recombinant DNA was recovered from the 1 phage by in vivo excision as pBluescript plasmid DNA. The size of the recombinant DNA ranged from 2-7kb to 2.9 kb, and sequence analysis revealed that all four clones cont ...
Regulation of the S100B gene by α1-adrenergic - AJP
... (PKC) signaling pathway. In the present study, we examined whether the same pathway induced the S100B gene, supporting the hypothesis that S100B is a feedback negative regulator of this pathway. We transfected cultured neonatal rat cardiac myocytes with a luciferase reporter gene driven by the maxim ...
... (PKC) signaling pathway. In the present study, we examined whether the same pathway induced the S100B gene, supporting the hypothesis that S100B is a feedback negative regulator of this pathway. We transfected cultured neonatal rat cardiac myocytes with a luciferase reporter gene driven by the maxim ...
No Slide Title
... If a mutation found in one of the HNPCC genes then sequential testing of the family can be performed. If Jane’s family declines genetic testing then family members should follow high risk screening recommendations for CRC. ...
... If a mutation found in one of the HNPCC genes then sequential testing of the family can be performed. If Jane’s family declines genetic testing then family members should follow high risk screening recommendations for CRC. ...
RNA-Seq
RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.