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Salmonella typhimurium
Salmonella typhimurium

... This Salmonella typhimurium Gene Database is UniProt-centric in that the main data source (primary ID System) for gene IDs and annotation is the UniProt complete proteome set for Salmonella typhimurium, made available as an XML download by the Integr8 resource. In addition to UniProt IDs, this datab ...
Origin of Mutations in Two Families With X-Linked
Origin of Mutations in Two Families With X-Linked

... neutrophils that were positive in the N B T slide test. Numbers greater than 95% represent the normal control range. Affected males are unable to reduce NBT (0% positive). Females with 50% (Fig l ) , or 32%, 53%, and 35% (Fig 2) positive cells were diagnosed as probable heterozygotes. It is notewort ...
Genomic disorders: structural features of the genome can lead to
Genomic disorders: structural features of the genome can lead to

... disease, and with Ingram’s demonstration of a specific chemical difference between the hemoglobins of normal and sickled human red blood cells. During the four decades that followed, investigations have focused on the gene – how mutations specifically alter DNA and how these changes affect the struc ...
Gregor Mendel Garden Pea Monohybrid Cross
Gregor Mendel Garden Pea Monohybrid Cross

PDF
PDF

... silencing and developmental functions may be linked (see below). However, in some cases, genetic studies have indicated exclusive roles in either silencing or developmental control (Morel et al. 2002), keeping open the possibility that Argonaute proteins may exert some of their biological functions ...
Genetic Testing for Cancer Susceptibility
Genetic Testing for Cancer Susceptibility

... The individual undergoing genetic testing will receive genetic counseling (if checked, complete below) The testing is being offered in a setting with adequately trained health care professionals to provide appropriate pre- and post-test counseling Other (please describe): ...
An Introduction to Bioconductor_2014
An Introduction to Bioconductor_2014

... Now we are familiar with the data, we could go the next step and do the analysis... ...
The History of Molecular Biology
The History of Molecular Biology

... that must provide the basis of evolution... It was generally assumed that genes would be composed of amino acids because, at that time, they appeared to be the only biomolecules with sufficient complexity to convey genetic information. This hypothesis is eventually dead from its shaking base and att ...
Hereditary Breast Cancer 5 061113
Hereditary Breast Cancer 5 061113

... A man in your family has had breast cancer A family member with a known BRCA1 or BRCA2 mutation Personal or family history of ovarian/fallopian tube/primary peritoneal cancer at any age Personal history of “triple negative” (ER neg, PR neg, Her2neu neg) breast cancer at or before age 60 ...
Ch.15 Study Guide
Ch.15 Study Guide

... crossing over has occurred. During prophase of meiosis I, paired homologous chromosomes break at corresponding points and switch fragments, creating new combinations of alleles that are then passed on to the gametes.  Geneticists can use recombination data to map a chromosome’s genetic loci (pp. 27 ...
The genetic basis of evolutionary change in gene expression levels
The genetic basis of evolutionary change in gene expression levels

... the evolution of form as spectacularly evidenced through the study of development (Carroll 2008), the relationship between genetics and development was at one time a yawning gulf. The rise of the authority of Mendelian genetics ushered in by T.H. Morgan led to a view where genes were treated as trai ...
Presentation 1 Guidelines
Presentation 1 Guidelines

... C7. The bases conform to the AT/GC rule of complementarity. There are two hydrogen bonds between A and T and three hydrogen bonds between G and C. The planar rings of the bases stack on top of each other within the helical structure to provide even more stability. C8. 3–CCGTAATGTGATCCGGA–5 C9. The ...
Red Line Walk-through
Red Line Walk-through

... UniGene: A database of transcript data, “each UniGene entry is a set of transcript sequences that appear to come from the same transcription locus (gene or expressed pseudogene), together with information on protein similarities, gene expression, cDNA clone reagents, and genomic location.” http://ww ...
'This day designing God Hath put into my hand
'This day designing God Hath put into my hand

... 1955 and resulted in the subsequent elimination of malaria from Europe, most of the Asian regions of Russia, the United States of America and most of the Caribbean (Krogstad, 1996). However, the eradication programme has been abandoned since it became obvious that malaria cannot be dealt with as a s ...
Genetics and Epigenetics of Human Disease
Genetics and Epigenetics of Human Disease

... is silenced (switched off). Another disease, Angelman syndrome, confirmed that some human genes are normally subject to genomic imprinting, a phenomenon in which a gene is silenced depending on whether it was inherited from father or from mother. The molecular silencing process (DNA methylation) inv ...
A simple and improved PCR-based technique for white
A simple and improved PCR-based technique for white

... and Mindell 2000), where one allele contains a restriction site around the SNP and the other does not. PCR amplification followed by restriction digestion can then be used to differentiate XX individuals from XY individuals (Fernando and Melnick 2001). Optimally sex-identification should be accompli ...
Bioinformatics and genomics PPT - BLI-Research-Synbio
Bioinformatics and genomics PPT - BLI-Research-Synbio

... 1. Develop tools to allow for efficient access and management of databases. 2. Analyze and make sense of a large amount of DNA and proteins sequences; ex. Gene identification, predict protein structure and function, and conduct evolutionary analyses. 3. Develop new programs for the utilization and m ...
Genotype to phenotype: lessons from model organisms
Genotype to phenotype: lessons from model organisms

... Non-coding variation. An important challenge that also deserves more attention is to predict the effects of mutations in non-protein-coding regions of the genome: for example, when do mutations in regulatory regions affect phenotypic variation? This is particularly important, given the accumulating ...
Using gene networks to drug target identification
Using gene networks to drug target identification

... inhibition or activation on multiple signaling pathways. Graphical models are often used to describe genetic networks. Generally, a gene network could be presented in a directed graph, in which nodes indicate genes and edges represent regulations between genes (e.g. activation or suppression). Analy ...
Transient Overexpression of Recombinant Proteins in Mammalian
Transient Overexpression of Recombinant Proteins in Mammalian

... ultra­high expression of your protein of interest. Both Vector I and Vector II systems incorporate the TAR sequence in the 5' untranslated region of the target gene, where it will not affect the amino acid sequence of the expressed target protein. For the Vector I system, a Tat cassette is included  ...
Abstract - BioMed Central
Abstract - BioMed Central

... therefore do not eventuate in KS subgraphs. This successfully filters out the majority of false pairwise similarities that were detected by using a low score threshold. At this stage all true CBGs can be correctly retrieved. This is only valid for genecontaining sequences that have a high level of p ...
Transcription response in the TGF-beta pathway Francisco Manuel
Transcription response in the TGF-beta pathway Francisco Manuel

... sequencing chemistry. The first step prior to sequencing is the library preparation. Adaptor sequences  are ligated to the DNA fragments. The ligated fragments are then amplified and immobilized in a flow  cell surface, where they are directly amplified (solid phase amplification) to create up to 10 ...
Dragon Genetics
Dragon Genetics

... The Law of Independent Assortment activities help students to understand that genes located on two separate chromosomes are inherited independently. First, the basis for understanding the Law of Independent Assortment is developed by analyzing expected outcomes of meiosis and fertilization. Then, a ...
Glossary - ChristopherKing.name
Glossary - ChristopherKing.name

... most current sequence information for genetic loci. Gene – an NCBI database of genetic loci. It may be accessed through the NCBI homepage by selecting “Gene” from the Search drop-down menu. This database used to be called LocusLink. Entries provide links to RefSeqs, articles in PubMed, and other des ...
Chapter 6A
Chapter 6A

... produced from a given mRNA. However, multiple forms of mRNAs, and therefore proteins, are produced from many genes. Simple gene transcription units produce only one type of mRNA and protein (Fig. 6.3a). Mutations at sites a & b often reduce or prevent transcription. Mutations at site c can change th ...
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RNA-Seq



RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.
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