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Applied and Environmental Microbiology
Applied and Environmental Microbiology

... and L. ivanovii (1, 18) are considered as pathogens. The pathogenicity is closely associated with a virulence gene cluster, although other genes like those coding for internalines are implicated in pathogenesis too (16). Like L. monocytogenes and L. ivanovii, L. seeligeri also carries a virulence ge ...
detection of mstn polymorphism in rabbit detectarea polimorfismului
detection of mstn polymorphism in rabbit detectarea polimorfismului

Modulation of CTCF Insulator Function by
Modulation of CTCF Insulator Function by

... CTCF from its occupancy site by chromatin immunoprecipitation after LPS stimulation. Moreover, RNAi-mediated depletion of CTCF leads to earlier onset of LPS-induced lysozyme gene expression. Taken together, these results suggest that the upregulation of lysozyme expression is mediated in part by the ...
Transferrin receptor 2 (TfR2) and HFE mutational analysis in non
Transferrin receptor 2 (TfR2) and HFE mutational analysis in non

... ver, BC, Canada, for genetic testing for HH prior to May 5, 2000. From this database, we selected all patients with a transferrin saturation greater than or equal to 60%, an elevated serum ferritin level, and a negative test result for the C282Y HFE mutation. From the same laboratory, 93 control sam ...
Genetically Modified Zoysia Grass (Zoysia japonica Steud
Genetically Modified Zoysia Grass (Zoysia japonica Steud

... auxin picloram of the afb5 mutant. Complementation studies and biochemical analysis were also performed. The rms branching mutants, rather than being depleted in IAA levels, contained elevated IAA levels. We proposed a model where a non-response to SLs, which occurs in SL-biosynthesis or in SL-respo ...
Bio-Tech - AgriLife Extension County Offices
Bio-Tech - AgriLife Extension County Offices

... Scientists have discovered that each gene carries the code for a specific, assigned function (such as whether a pea is wrinkled or smooth).Genetic engineering is the process in which a specific gene is taken from the DNA of one organism and inserted into the DNA of another, or a specific gene is rep ...
GENE REGULATION AT THE PROMOTER LEVEL
GENE REGULATION AT THE PROMOTER LEVEL

... All cells use only a fraction of their total number of genes (their “genome’) at a given time. Gene expression is an expensive process, it takes a lot of energy to produce mRNA and protein and also a lot of often limiting nutrients such as N and P. It would be wasteful, for example, for a bacterium ...
Germ Line Transmission and Expression of a Corrected HPRT Gene
Germ Line Transmission and Expression of a Corrected HPRT Gene

... the mammalian genome would be of benefit both to biological and medical research. It would become possible to manipulate the expression of genes by targeting changes to their control sequences. This would be of value to the study of gene expression. It could also be of potential commercial value if ...
Procedure - DNA Interactive
Procedure - DNA Interactive

... precision of automated thermal cycling. However, amplification of bz-2 locus is not complicated by the presence of repeated units. Therefore, the recommended amplification times and temperatures will work adequately for all types of thermal ...
Gill: Gene Regulation II
Gill: Gene Regulation II

... 4C example result (in a single biological context) TSS probe ...
PLoS One
PLoS One

Lecture 14
Lecture 14

... promoter specificity and therefore produces transcripts which have been initiated and terminated at random. Today, RNA polymerases from a number of phages are used, which possess a high degree of specificity. Thus transcription can be limited to sequences cloned downstream of an ...
Two genes from Bacillus subtilis under the sole control
Two genes from Bacillus subtilis under the sole control

... Computer analysis. Database searches and sequence alignments were done using the gapped BLAST program of Altschul et al. (1997). ...
A GO annotation is
A GO annotation is

... continually being created - always use a current version of both - if publishing your analyses please report the versions/dates you used http://www.geneontology.org/GO.cite.shtml ...
Supplemental File: Detailed Clinical Description, Sequencing
Supplemental File: Detailed Clinical Description, Sequencing

... several large (>10 Mb) regions of homozygosity that include hundreds of potential gene candidates were identified (Supplemental Table 2, see below), as is consistent with their known consanguinity. ...
Evolution of RH Genes in Hominoids: Characterization of a Gorilla
Evolution of RH Genes in Hominoids: Characterization of a Gorilla

... and RhcE polypeptides were 29 and 25, respectively ( Table 2). Differences between gorilla RHCE-like polypeptide and two other gorilla Rh-like polypeptides were almost identical (24 and 25, respectively) ( Table 2). The topology of the phylogenetic tree obtained from the alignment shown in Figure 4 ...
Supporting Online Material
Supporting Online Material

... Figure S9. Scheme of crossing for silencing of dUTPase in the dorsal compartment of Drosophila wing imaginal discs. Crossing scheme is shown on panel (A): virgin females of the MS1096 Gal4 enhancer trap line expressing Gal4 preferentially in the dorsal compartment of the wing and carrying UAS-Dicer2 ...
Mendel Genetics/Genetics Intro
Mendel Genetics/Genetics Intro

... F2 (2nd generation offspring) Both purple and white offspring resulted in the second (F2) generation - but 3 times more purple than white. In other words, the ratio of purple to white was 3:1. TERM: F2 generation - offspring of a cross between two F1 ...
Densovirus infection in silkworm Bombyx mori and genes
Densovirus infection in silkworm Bombyx mori and genes

... The genetic make-up of DVs can be classified mainly into two types: The ambisense DVs that encode open reading frames (ORFs) on both complimentary strands, while the monosense DVs that has only a single strand containing the ORFs. Overall DVs can be described as viruses having small isometric, non-e ...
Frequent Association of p53 Gene Mutation in
Frequent Association of p53 Gene Mutation in

... biological behavior of cancers. For example, c-erbB-2 or K-sawi specimens. The region of exon 9 was analyzed by PCR-SSCP with the amplification correlated well with biological malignancy in primers as described above. Mobility shifts were detected in gastric cancer (25, 26) as well as amplification ...
Genetic tasks V: GENE INTERACTIONS
Genetic tasks V: GENE INTERACTIONS

... 7. The length of rabbit ears is determined by three genes A, B, C. Recessive homozygotes in all three genes have ears 10 cm long and is longer for 2 cm with presence of other dominant allele. What ear length and phenotype ratio could be expected in offspring resulting from crossing of aaBbCc × AABb ...
[Full text/PDF]
[Full text/PDF]

... testing [18], one of the major concerns is that these programs are incapable of scale-up for analyzing GWAS data, as they were not designed with genome-wide data in mind and thus could fail owing to memory and disk usage issues [6]. However, even though the MDR and its extensions are incapable of ha ...
Börjeson–Forssman–Lehmann syndrome: defining
Börjeson–Forssman–Lehmann syndrome: defining

Simultaneous mutation scanning for gross deletions
Simultaneous mutation scanning for gross deletions

... streamlined approach. Fluorescent multiplex quantitative PCR is used to determine the copy number of each exon, followed by conformation sensitive capillary electrophoresis (CSCE) of the same PCR products on a multi-capillary genetic analyser. We have developed this technique to screen all 79 exons ...
Lecture_8
Lecture_8

... Both methods generate labeled fragments of varying lengths that are further electrophoresed. ...
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RNA-Seq



RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.
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