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Sample COLARIS AP LMNs
Sample COLARIS AP LMNs

... I am writing to request coverage for the cost of this patient’s analysis for known familial mutations in the MYH gene. Biallelic mutations in the MYH gene are associated with multiple colorectal adenomas and a high risk of colorectal cancer. In addition, patients may present with extracolonic diseas ...
Solid Tumour Section Soft tissue tumors: Dermatofibrosarcoma protuberans Atlas of Genetics and Cytogenetics
Solid Tumour Section Soft tissue tumors: Dermatofibrosarcoma protuberans Atlas of Genetics and Cytogenetics

... of the recurrent t(17;22)(q22;q13) translocation or, more commonly, supernumerary ring chromosomes containing material from chromosomal regions 17q22 and 22q13 accompanied by simple chromosome trisomies. The translocation results in the fusion of the alpha chain type 1 of collagen (COL1A1) gene with ...
Gene targeting in filamentous fungi: the benefits of impaired repair
Gene targeting in filamentous fungi: the benefits of impaired repair

... integration in NHEJ-deficient genetic backgrounds were published, such as for the human pathogens Aspergillus fumigatus or Cryptococcus neoformans, the model organism Aspergillus nidulans, the koji molds Aspergillus oryzae and Aspergillus sojae, and the filamentous ascomycete Sordaria macrospora (Ta ...
DNA Sequencing by Targeting 16S rRNA Gene for Novel Strain
DNA Sequencing by Targeting 16S rRNA Gene for Novel Strain

... diversity within the targeted DNA regions. ...
Off-target Effects: Disturbing the Silence of RNA
Off-target Effects: Disturbing the Silence of RNA

... Off-target effects occur when an siRNA is processed by the RNA-Induced Silencing Complex (RISC) and down-regulates unintended targets. As these changes in gene expression can lead to measurable phenotypes (such as false positives) it is of great importance to understand the mechanism behind off-targ ...
Genetics CH 6 Test 2011
Genetics CH 6 Test 2011

... PART A: MULTIPLE CHOICE [K/U: 1 mark each = 5 marks] Circle the choice that best answers the question. 1. A test cross is one in which the organism with the unknown genotype is mated with an organism that is a. heterozygous for the trait b. homozygous dominant for the trait c. homozygous recessive f ...
Correlating mRNA and protein Abundance
Correlating mRNA and protein Abundance

... Ratios of wt+gal to wt gal ICAT vs microarray N ~ 290, r = 0.6 ...
Genome Analysis Excerpt from Chapter 11
Genome Analysis Excerpt from Chapter 11

... There are several classes of sequences (transposable elements) that can move from one genome location to another, thus affecting gene content. Highly repetitive sequences in the genome are derived from such classes of sequences that move (transpose) from one genome location to another. These sequenc ...
Conserved amino acid sequences confer nuclear localization
Conserved amino acid sequences confer nuclear localization

... derived from pituitary gland RNA. The putative second intron of the gene was first amplified from genomic DNA using primers based upon porcine and human sequences (Wu et al., 1998; Sloop et al., 2000). This sequence information was then used to design primer pairs allowing amplification of the entir ...
Expansion of specialized metabolism
Expansion of specialized metabolism

... grapevine (Vitis vinifera) (Jaillon et al. 2007), soybean (Glycine max) (Schmutz et al. 2010) and rice (Oryza sativa) (Ouyang et al. 2007), which are not only agronomically important crops but also represent the major taxonomic group of angiosperms. Lycophyte (Selaginella moellendorffii) (Banks et a ...
Unit 3
Unit 3

... (round or wrinkled) AND plant height (tall or short) Set up is the same for the punnett square Keep the alleles for each trait together in the boxes!! ...
Gene Section RAP1B (RAP1B, member of RAS oncogene family)
Gene Section RAP1B (RAP1B, member of RAS oncogene family)

... Rap1 is bound to membranes. In many cell types, it is found in a perinuclear compartment overlapping the Golgi. Rap1 proteins (A and B) are phosphorylated near the C-ter by cAMP-dependent protein kinase. This results in translocation of part of the Rap1 pool to the cytosol. ...
Plasmodium malariae and P. ovale genomes provide insights into
Plasmodium malariae and P. ovale genomes provide insights into

... evolutionary relationship of these species to the other humaninfective species has been contested6,7. Using a new reference genome for P. malariae and a manually curated draft P. o. curtisi genome, we are now able to accurately place these species within the Plasmodium phylogeny. Sequencing of a P. ...
CHAPTER 4: CELLULAR METABOLISM
CHAPTER 4: CELLULAR METABOLISM

... Catalysts: Enzymes in biological systems. Metabolic pathways involve several reactions in a row, with each reaction requiring a specific enzyme. See Fig. 4.5, page 107. Enzyme names are often derived from the substrate that they act upon (providing the root of enzyme name), and the enzyme names typi ...
PDF file
PDF file

Exonic and Intronic Sequence Variation in the Human Leptin
Exonic and Intronic Sequence Variation in the Human Leptin

... which have been previously reported (nt 519/LyslO9Arg, nt 861/Gln223Arg, and nt 3250) (15). Of the three nucleotide alterations that produce amino acid changes, Gln223Arg and Lys656Asn result in changes in charge (neutral to positive and positive to neutral, respectively) and are, therefore, the mos ...
Scientists Say Human Genome Is Complete
Scientists Say Human Genome Is Complete

... genetic data banks. Celera, whose data are available by subscription, never intended to carry its draft genome to completion. The working draft of three years ago contained most human genes and was useful for researchers seeking a specific gene. But up to a year ago biologists said they often had to ...
AP Biology
AP Biology

... In the 1990’s when scientists began to compile a list of genes and DNA sequences in the human genome it became abundantly clear that we were eventually going to need a place to put all of these sequences. One of the systems developed was BLAST, or Basic Local Alignment Search Tool. The BLAST compute ...
Evolution of antifreeze glycoprotein gene from a trypsinogen gene in
Evolution of antifreeze glycoprotein gene from a trypsinogen gene in

... notothenioids, D. mawsoni, N. coriiceps, and Pagothenia borchgrevinki was amplified for AFGP gene sequences by PCR using as primers the 59 untranslated region (UTR) sequence (cTrypAF59), and a 30-nt 39 primer (cTryp-AF39) that anneals to a site immediately ahead of the putative polyadenylylation sig ...
Case of the Hooded Murder
Case of the Hooded Murder

... found a witness to the murder, a maid who heard a groan from the library. Afraid to go in, she had peep through the keyhole and seen someone in a long hooded cape. ...
Genomic instability — an evolving hallmark of cancer
Genomic instability — an evolving hallmark of cancer

... frequency of mutations is still higher, perhaps because the primary tumours in these studies were examined after being propagated as cell lines or xenografts24–27. Differences in the methods used to identify mutations could also account for the difference in mutation fre­ quencies as the methods use ...
Inheritance The passing of traits from parents to offspring Genetics
Inheritance The passing of traits from parents to offspring Genetics

... -Extranuclear genes are those found in the mitochrondria and chloroplasts. They were discovered in plants in 19009. Since then, they have been linked to several rare and severe inherited diseases in humans. Defects in the mitochrondrial DNA reduce the amount of ATP a cell can make, Therefore the org ...
Genetics Notes - Cobb Learning
Genetics Notes - Cobb Learning

... – Dominant - A gene that is always expressed and hides others – Recessive - A gene that is only expressed when a dominant gene isn’t present – Codominant - Genes that work together to produce a third trait ...
Heredity
Heredity

... working with sex-linked genes because when females (XX) inherit a sex-linked gene, they receive two copies of the gene, one on each X chromosome. • In contrast, a male (XY) will inherit only one copy of the gene because only the X chromosome delivers the gene. • Whichever allele is on the X chromoso ...
Carroll 2006 Fossil Genes
Carroll 2006 Fossil Genes

... are also vital to animal behavior and survival, particularly the sense of smell. O n e walk in the park with a dog provides many examples of how their "view" of the world is shaped by their acute sense of smell. Many other mammals also have powerful senses of smell, which are used t o find food, ide ...
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RNA-Seq



RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.
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